scholarly journals Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods

2019 ◽  
Vol 56 (12) ◽  
pp. 5386-5395 ◽  
Author(s):  
Katarzyna Pobiega ◽  
Karolina Kraśniewska ◽  
Dorota Derewiaka ◽  
Małgorzata Gniewosz
Keyword(s):  
Antimicrobial Activity ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenol Content ◽  
Ultrasound Assisted Extraction ◽  
Disk Diffusion Method ◽  
Propolis Extract ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Abstract The objective of the study was to compare the antimicrobial activities of ethanolic propolis extracts obtained using different extraction methods. Extraction of propolis was carried out using 70% ethanol, propolis to ethanol ratios of 1:10 and 1:5, extraction times of 1 or 7 days, and shaking extraction (SE), ultrasound-assisted extraction (UAE), and ultrasound-assisted shaking extraction (SUAE) methods. A total of 12 propolis extract lyophilizates were obtained. Samples were tested for extraction yield and for total phenol content by the Folin–Ciocalteau colourimetric method, and total flavonoid content using a spectrophotometric method. GLC/MS was used for the identification of chemical compounds in selected extract lyophilizates. Antimicrobial activity against selected bacterial and fungal species was assessed using the disk diffusion method. Propolis extracts obtained as the result of 1-day and 7-day shaking extraction followed by 20 min of ultrasound-assisted extraction (SUAE) had better antimicrobial properties as compared to those obtained by SE or UAE alone. SE and UAE gave lower extraction yields as well as lower phenol and flavonoid contents compared to SUAE. No differences were observed with regard to the qualitative composition of extracts obtained by any of the methods. It is best to obtain the extract using the combined method of 1-day extraction and 20-min sonication.

scholarly journals Effects of Ultrasound-Assisted Extraction and Solvent on the Phenolic Profile, Bacterial Growth, and Anti-Inflammatory/Antioxidant Activities of Mediterranean Olive and Fig Leaves Extracts

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1718 ◽  
Author(s):  
Cristina Alcántara ◽  
Tihana Žugčić ◽  
Radhia Abdelkebir ◽  
Jose V. García-Pérez ◽  
Anet Režek Jambrak ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Bacterial Growth ◽  
Extraction Methods ◽  
Olive Leaves ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Profile ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
Anti Inflammatory

Mediterranean plants, such as fig and olive leaves, are well-known to exert beneficial effects in humans because of the presence of a wide range of bioactive compounds. However, scarce information regarding the impact of extraction methods, such as ultrasound and types of solvents, on their profile of antioxidant and anti-inflammatory compounds is provided. In addition, no information is available on the effects of extraction methods and solvents on the inhibition of pathogenic bacteria or promoting probiotic growth. In this scenario, this study was aimed to study the effects of ultrasound-assisted extraction (UAE) and solvent on the phenolic profile (Triple TOF-LC-MS/MS), antioxidant and anti-inflammatory compounds of olive and fig leaves. Results showed that UAE extracted more carotenoids compared to conventional extraction, while the conventional extraction impacted on higher flavonoids (olive leaves) and total phenolics (fig leaves). The antioxidant capacity of aqueous extract of fig leaves was three times higher than the extract obtained with ethanol for conventional extraction and four times higher for UAE. In general terms, hydroethanolic extracts presented the highest bacterial growth inhibition, and showed the highest anti-inflammatory activity. In conclusion, these side streams can be used as sources of bioactive compounds for further development of high-added-value products.

Antimicrobial activity of raw soybean, soybean flour and roasted soybean extracted by ethanol-hexane method

2017 ◽  
Vol 119 (10) ◽  
pp. 2277-2286 ◽  
Author(s):  
Sireerat Laodheerasiri ◽  
Nirasha Horana Pathirage
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Compounds ◽  
Soybean Flour ◽  
Hexane Extraction ◽  
Disk Diffusion Method ◽  
Content Type ◽  
E Coli ◽  
Inhibitory Compounds

Purpose The purpose of this paper is to determine the antimicrobial compounds extracted from various types of soybean materials; raw soybean, soybean flour and roasted soybean. Bacteria growth inhibition was tested in E.coli and S. aureus by disk diffusion method. Efficiency of antimicrobial activities were compared among the extracted solution. Design/methodology/approach Soybean (Glycine max) isoflavones contain the ability to inhibit the growth of many strains of microorganisms. The solid-liquid extraction, ethanol-hexane extraction, was modified to isolate the inhibitory compounds from the three different types of soybean materials. All crude extracts at various concentrations performed under different extracted solutions (75, 50, 25, 12.5, 6.25 and 3.125 percent) were tested with E. coli. and S. aureus to determine the antimicrobial activities and the minimum inhibition concentration (MIC). Disk diffusion method was chosen to study the antimicrobial activity of isoflavones. Findings Soybean flour extract inhibited the growth of E. coli and S. aureus more effective than roasted soybean and raw soybean, respectively. The MIC of inhibitory compounds extracted from soybean flour was 6.25 percent (0.031 mg/ml), roasted soybean was 25 percent (0.125 mg/ml) and raw soybean was 50 percent (0.250 mg/ml). Crude extracts of soybean flour and roasted soybean showed better results than raw soybean. All the three of extracted soybeans inhibited the growth of E. coli better than S. aureus. Originality/value Ethanol-hexane extraction was successfully used to isolate the antimicrobial compounds from raw soybean, soybean flour and roasted soybean. Comparison of antimicrobial activity showed that soybean flour contained the highest inhibition activity.

scholarly journals Simultaneous Determination of REEs in Coal Samples Using the Combination of Microwave-Assisted Ashing and Ultrasound-Assisted Extraction Methods Followed by ICP-OES Analysis

Minerals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1103
Author(s):  
Mceliseni Zuma ◽  
Philiswa Nomngongo ◽  
Nomvano Mketo
Keyword(s):  
Inductively Coupled Plasma ◽  
Bath Temperature ◽  
Extraction Methods ◽  
Icp Oes ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The world during the COVID-19 pandemic has led to extensive use of virtual activities by means of electronic devices, which are made up of rare earth elements (REEs). This means that quantitative knowledge of REEs in various resources is crucial for the development of effective recovery methods. Therefore, this report describes a simple microwave assisted ashing followed by ultrasound-assisted extraction (MAA-UAE) for quantitative determination of REEs in coal samples using inductively coupled plasma-optical emission spectroscopy (ICP-OES). Firstly, coal samples were ashed at 55 °C for 4.5 h to form white ashes, which were then treated with dilute HNO3 acid under ultrasonication to enhance the extraction of REEs. The quantitative recoveries (86–120%) of REEs were obtained when 1 mol L−1, 0.1 g, 40 °C, 20 min, and high frequency were applied for [HNO3], sample mass, ultrasonic bath temperature, extraction time, and ultrasonic bath frequency, respectively. The method detection limits of the proposed MAA-UAE method were between 0.0075 and 0.59 µg g−1 with satisfactory precision (<5%). The concentration levels of REEs in South African coals ranged from 1.4 to 105 µg g−1, suggesting that this coal can be a resource for REEs.

scholarly journals Ultrasound-Assisted Extraction of Two Types of Antioxidant Compounds (TPC and TA) from Black Chokeberry (Aronia melanocarpa L.): Optimization of the Individual and Simultaneous Extraction Methods

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 456 ◽  
Author(s):  
Mercedes Vázquez-Espinosa ◽  
Ana V. González-de-Peredo ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
Juan José Toledo-Domínguez ◽  
...  
Keyword(s):  
Volume Ratio ◽  
Extraction Methods ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Extraction Solvent ◽  
Aronia Melanocarpa ◽  
Assisted Extraction ◽  
Black Chokeberry ◽  
Ultrasound Assisted ◽  
The Individual

Chokeberry (Aronia melanocarpa L.) contains a large number of bioactive compounds, which make of it a highly nutritional and antioxidant food with anti-cancer and anti-inflammatory properties. It has been recently seen its consumption increased because of its interesting composition and properties as a functional food. The ultrasound-assisted extraction method has been optimized, since it is a simple and fast technique to extract phenolic compounds, as well as anthocyanins, not only separately but also simultaneously. Multiple response has been optimized and demonstrated an effectiveness level similar to the individual ones. This represents a considerable reduction in costs, time and work. The optimal conditions for simultaneous extractions were: 54% methanol as extraction solvent at pH 2.72 and 69.4 °C temperature, 70% amplitude, 0.7 s cycle, and 0.5:18.2 g:mL sample mass/solvent volume ratio. The developed methods showed a high precision level with coefficients of variation lower than 5%. The methods were successfully applied to commercial samples. Additionally, the extraction of other compounds of biological interest, such as organic acids and sugars, was also studied.

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

2019 ◽  
Vol 66 (3) ◽  
pp. 361-368 ◽  
Author(s):  
Abdalla Ali Salim ◽  
Sanja Grbavčić ◽  
Nataša Šekuljica ◽  
Maja Vukašinović‐Sekulić ◽  
Jelena Jovanović ◽  
...  
Keyword(s):  
Solid State ◽  
Comparative Study ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Enzyme Production ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Chemical Composition and Biological Activities of Extracts from Pomelo Peel By-Products under Enzyme and Ultrasound-Assisted Extractions

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Pham Van Hung ◽  
Nguyen Hai Yen Nhi ◽  
Ling Yu Ting ◽  
Nguyen Thi Lan Phi
Keyword(s):  
Chemical Composition ◽  
Antimicrobial Activities ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
By Products ◽  
Enzyme Assisted Extraction ◽  
Antioxidant And Antimicrobial Activities

Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) were popular methods used to extract bioactive compounds from citrus peels, by-products of fruit processing industry. In this study, the total phenolic content (TPC), total flavonoid content (TFC), naringin and hesperidin contents, and antioxidant and antimicrobial activities of the extracts from pomelo peels using the combined enzyme and ultrasound-assisted extraction (E-UAE) or ultrasound and enzyme-assisted extraction (U-EAE) technique were investigated and compared with those extracted using the EAE and UAE. The optimal EAE conditions were as follows: enzyme concentration of 2%, water-solid ratio of 40 ml/g, incubation temperature of 50°C, and extraction time of 60 min, whereas the optimal UAE conditions were ultrasonic energy of 40 kHz, water-solid ratio of 40 ml/g at room temperature, and extraction time of 60 min. The results indicate that the total phenolics, total flavonoids, naringin, and hesperidin contents of the extracts significantly increased in the following order of the extraction techniques: UAE < EAE < U-EAE < E-UAE (p<0.05). The combined E-UAE technique was the most effective technique for bioactive compound extraction with the highest antioxidant and antimicrobial activities. The results also indicate that chemical composition and antioxidant and antimicrobial activities of the extracts were different depending on the pomelo species.

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