Two-step optimization of solid-state fermentation conditions of heilong48 soybean variety for maximum chlorogenic acid extraction yield with improved antioxidant activity

2021 ◽  
Vol 168 ◽  
pp. 113565
Author(s):  
Nelson Dzidzorgbe Kwaku Akpabli-Tsigbe ◽  
Yongkun Ma ◽  
John-Nelson Ekumah ◽  
Juliet Osabutey ◽  
Jie Hu ◽  
...  
Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1518
Author(s):  
De León-Medina José Carlos ◽  
Sepúlveda Leonardo ◽  
Morlett-Chávez Jesús ◽  
Meléndez-Renteria Paola ◽  
Zugasti-Cruz Alejandro ◽  
...  

This work was performed to study Castilla Rose (Purshia plicata) as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism Aspergillus niger GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made. Finally, the antioxidant activity of the recovered compounds was tested by ABTS, DPPH, and lipid oxidation inhibition assays. The best fermentation conditions were temperature 25 °C and inoculum 2 × 106 spores/g, while the maximum extraction time of polyphenols was 24 h (173.95 mg/g). The HPLC/MS analysis allowed the identification of 25 different polyphenolic compounds, and the antioxidant activity of the obtained polyphenols was demonstrated, showing ABTS assay the most effective with inhibition of 94.34%.


Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4832
Author(s):  
Jia Wei Peng ◽  
Ho Shing Wu

In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.


2019 ◽  
Vol 207 (1) ◽  
pp. 31-42 ◽  
Author(s):  
José Lucas de Almeida Antunes Ferraz ◽  
Lucas Oliveira Souza ◽  
André Gustavo de Araújo Fernandes ◽  
Marcio Luiz Ferreira Oliveira ◽  
Julieta Rangel de Oliveira ◽  
...  

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1683
Author(s):  
Elvyra Jariene ◽  
Marius Lasinskas ◽  
Honorata Danilcenko ◽  
Nijole Vaitkeviciene ◽  
Alvyra Slepetiene ◽  
...  

At present, the consumption of medical plants and functional foods is growing in the whole world. Rosebay willowherb (Chamerionangustifolium (L.) Holub) is an important medicinal plant that has various pharmacological effects (antioxidant, anti-inflammatory, anticancer, and others), can improve the state of health and well-being, and reduce the risk of various diseases. The aim of this work was to investigate volatile compounds, polyphenols, and antioxidant activity in rosebay willowherb leaves fermented for 24 and 48 h in solid state fermentation under aerobic and anaerobic conditions. High-performance liquid chromatography (HPLC) for polyphenols and the spectrophotometric method for antioxidant activity determinations were used. To recognize and identify the leaves’ fragrances, electronic nose (Alpha M.O.S) measurement technology was used. The results showed that the highest amounts of total polyphenols in dried matter were after 48 h aerobic solid state fermentation (SSF). Antioxidant activity was higher under 48 h SSF compared to the control. The most abundant flavoring compound groups were esters, terpenes, and aldehydes. In unfermented leaves, (z)-3-hexen-1-ol, acetate, hexyl acetate, and trans-hex-2-enyl acetate prevailed, characterized by fragrances of greenery, flowers, and fruits. The undesired esters group compounds, ethyl butyrate and butyl acetate, with pungent odor, were detected after 48 h anaerobic SSF.


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