Storage-related changes of terpene constituents in caraway (Carum carvi L.) under real-time storage conditions

ABSTRACT Foodborne bacteria such as Escherichia coli O157:H7 can cause severe hemorrhagic colitis in humans following consumption of contaminated meat products. Contamination with pathogenic bacteria is frequently found in the food production environment, and adequate household storage conditions of purchased foods are vital for illness avoidance. Real-time monitoring was used to evaluate bacterial growth in ground horse, beef, and pork meats maintained under various storage conditions. Various levels of E. coli O157:H7 carrying the luxCDABE operon, which allows the cells to emit bioluminescence, were used to inoculate meat samples that were then stored at room temperature for 0.5 day, at 4°C (cold) for 7 or 9 days, or −20°C (frozen) for 9 days. Real-time bioluminescence imaging (BLI) of bacterial growth was used to assess bacterial survival or load. Ground horse meat BLI signals and E. coli levels were dose and time dependent, increasing during room temperature and −20°C storage, but stayed at low levels during 4°C storage. No bacteria survived in the lower level inoculum groups (101 and 103 CFU/g). With an inoculum of 107 CFU/g, pork meats had higher BLI signals than did their beef counterparts, displaying decreased BLI signals during 7 days storage at 4°C. Both meat types had higher BLI signals in the fat area, which was confirmed with isolated fat tissues in the beef meat. Beef lean and fat tissues contrasted with both pork fat and lean tissues, which had significantly higher BLI signals and bacterial levels. BLI appears to be a useful research tool for real-time monitoring of bacterial growth and survival in various stored livestock meats. The dependence of E. coli O157:H7 growth on meat substrate (fat or lean) and storage conditions may be used as part of an effective antibacterial approach for the production of safe ground horse, beef, and pork meats.

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Real Time Monitoring and Control of Inventory Storage Conditions for Grains

Indian Journal of Science and Technology ◽

10.17485/ijst/2019/v12i29/146967 ◽

2019 ◽

Vol 12 (29) ◽

pp. 1-5

Author(s):

P. Karthika Devi ◽

S. Balambigai ◽

T.C. Kalaiselvi ◽

◽

...

Keyword(s):

Real Time ◽

Storage Conditions ◽

Monitoring And Control ◽

Real Time Monitoring ◽

And Control

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Impact of Plastic Packaging Materials and Storage Time on the Quality Parameters of Lebanese Extra Virgin Olive Oil under Real-Time Storage Conditions

American Journal of Analytical Chemistry ◽

10.4236/ajac.2019.1012046 ◽

2019 ◽

Vol 10 (12) ◽

pp. 647-665

Author(s):

Carla Zarazir ◽

Mohamad Rajab ◽

Houssam Obeid ◽

Joumana Toufaily ◽

Imad Toufeili ◽

...

Keyword(s):

Olive Oil ◽

Real Time ◽

Storage Time ◽

Virgin Olive Oil ◽

Extra Virgin Olive Oil ◽

Storage Conditions ◽

Quality Parameters ◽

Packaging Materials ◽

Plastic Packaging ◽

And Storage

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Detection of SARS-CoV-2 by Real-Time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain

10.1101/2020.07.21.20158154 ◽

2020 ◽

Author(s):

Sabrina Summer ◽

Ralf Schmidt ◽

Anna Nele Herdina ◽

Isabella Krickl ◽

Julia Madner ◽

...

Keyword(s):

Real Time ◽

Health Care Providers ◽

Real Time Pcr ◽

Influenza A ◽

Storage Temperature ◽

Respiratory Viruses ◽

Storage Conditions ◽

Care Providers ◽

Viral Transport ◽

Syncytial Virus

AbstractWith global demand for SARS-CoV-2 testing ever rising, shortages in commercially available viral transport media pose a serious problem for laboratories and health care providers. For reliable diagnosis of SARS-CoV-2 and other respiratory viruses, executed by Real-time PCR, the quality of respiratory specimens, predominantly determined by transport and storage conditions, is crucial. Therefore, our aim was to explore the reliability of minimal transport media, comprising saline and the CDC recommended Viral Transport Media (HBSS VTM), for the diagnosis of SARS-CoV-2 and other respiratory viruses (influenza A, respiratory syncytial virus, adenovirus, rhinovirus and human metapneumovirus) compared to commercial products, such as the Universal Transport Media (UTM). We question the assumptions, that the choice of medium and temperature for storage and transport affect the accuracy of viral detection by RT-PCR. Both alternatives to the commercial transport medium (UTM), namely the CDC viral transport media (HBSS VTM) and saline, allow adequate detection of SARS-CoV-2 and other respiratory viruses, regardless of the storage temperature and time.Our study revealed the high resilience of SARS-CoV-2 and other respiratory viruses, enabling proper detection in clinical specimens even after long-time storage at high temperatures, independent of the transport medium’s composition.

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Optimized broad-range real-time PCR-based method for bacterial screening of platelet concentrates

Brazilian Journal of Biology ◽

10.1590/1519-6984.229893 ◽

2020 ◽

Author(s):

F. Alexandrino ◽

J. S. Malgarin ◽

M. A. Krieger ◽

L. G. Morello

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Limit Of Detection ◽

High Sensitivity ◽

Turnaround Time ◽

Storage Conditions ◽

Rrna Gene ◽

Platelet Concentrates ◽

Microbiological Methods ◽

Bacterial Screening

Abstract Bacterial contamination of blood components remains a major challenge in transfusion medicine, particularly, platelet concentrates (PCs) due to the storage conditions that support bacterial proliferation. In this study, we develop a rapid, sensitive and specific real-time PCR protocol for bacterial screening of PCs. An internally controlled real-time PCR-based method was optimized and validated with our proprietary 16S Universal PCR Master Mix (IBMP/Fiocruz), which targets a conserved region of the bacterial 16S rRNA gene. Nonspecific background DNA was completely eliminated by treating the PCR Master Mix with ethidium monoazide (EMA). A lower limit of detection was observed for 10 genome equivalents with an observed Ct value of 34±1.07 in calibration curve generated with 10-fold serial dilutions of E. coli DNA. The turnaround time for processing, including microbial DNA purification, was approximately 4 hours. The developed method showed a high sensitivity with no non-specific amplification and a lower time-to-detection than traditional microbiological methods, demonstrating it to be an efficient means of screening pre-transfusion PCs.

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Physicochemical Stability and Biological Activity ofWithania somniferaExtract under Real-Time and Accelerated Storage Conditions

Planta Medica ◽

10.1055/s-0029-1186220 ◽

2009 ◽

Vol 76 (05) ◽

pp. 481-488 ◽

Cited By ~ 12

Author(s):

Dada Patil ◽

Manish Gautam ◽

Umesh Jadhav ◽

Sanjay Mishra ◽

Suresh Karupothula ◽

...

Keyword(s):

Biological Activity ◽

Real Time ◽

Storage Conditions ◽

Accelerated Storage ◽

Physicochemical Stability

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HPLC Quantitative Analysis of Rhein and Antidermatophytic Activity ofCassia fistulaPod Pulp Extracts of Various Storage Conditions

BioMed Research International ◽

10.1155/2013/821295 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Savita Chewchinda ◽

Mansuang Wuthi-udomlert ◽

Wandee Gritsanapan

Keyword(s):

Quantitative Analysis ◽

Antifungal Activity ◽

Real Time ◽

Aluminum Foil ◽

Storage Conditions ◽

Broth Microdilution ◽

Cassia Fistula ◽

Initial Amount ◽

Antifungal Properties ◽

Antidermatophytic Activity

Cassia fistulais well known for its laxative and antifungal properties due to anthraquinone compounds in the pods. This study quantitatively analyzed rhein in theC. fistulapod pulp decoction extracts kept under various storage conditions using HPLC. The antifungal activity of the extracts and their hydrolyzed mixture was also evaluated against dermatophytes. The contents of rhein in all stored decoction extracts remained more than 95% (95.69–100.66%) of the initial amount (0.0823±0.001% w/w). There was no significant change of the extracts kept in glass vials and in aluminum foil bags. The decoction extract ofC. fistulapod pulp and its hydrolyzed mixture containing anthraquinone aglycones were tested against clinical strains of dermatophytes by broth microdilution technique. The results revealed good chemical and antifungal stabilities against dermatophytes ofC. fistulapod pulp decoction extracts stored under various accelerated and real time storage conditions.

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