scholarly journals Development of human alveolar epithelial cell models to study distal lung biology and disease

iScience ◽  
2022 ◽  
pp. 103780
Author(s):  
Evelyn Tran ◽  
Tuo Shi ◽  
Xiuwen Li ◽  
Adnan Y. Chowdhury ◽  
Du Jiang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Alveolar Epithelial Cell ◽  
Cell Models ◽  
Alveolar Epithelial ◽  
Distal Lung ◽  
Human Alveolar Epithelial Cell

Development of Novel Human Alveolar Epithelial Cell Models to Study Distal Lung Biology and Disease

2021 ◽  
Author(s):  
Evelyn Tran ◽  
Tuo Shi ◽  
Xiuwen Li ◽  
Adnan Y. Chowdhury ◽  
Du Jiang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Alveolar Epithelial Cell ◽  
Cell Models ◽  
Alveolar Epithelial ◽  
Distal Lung ◽  
Human Alveolar Epithelial Cell

scholarly journals Human Alveolar Epithelial Cell Injury Induced by Cigarette Smoke

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e26059 ◽  
Author(s):  
Beata Kosmider ◽  
Elise M. Messier ◽  
Hong Wei Chu ◽  
Robert J. Mason
Keyword(s):  
Epithelial Cell ◽  
Cigarette Smoke ◽  
Cell Injury ◽  
Alveolar Epithelial Cell ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cell ◽  
Epithelial Cell Injury

Effect of Steroids on the Synthesis of Complement C3 in a Human Alveolar Epithelial Cell Line

1993 ◽  
Vol 19 (5) ◽  
pp. 603-616 ◽  
Author(s):  
Terence L. Zach ◽  
Vicki A. Herrman ◽  
Laura D. Hill ◽  
M. Patricia Leuschen
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Alveolar Epithelial Cell ◽  
Complement C3 ◽  
Epithelial Cell Line ◽  
Alveolar Epithelial ◽  
Human Alveolar Epithelial Cell

Effect of stretch on structural integrity and micromechanics of human alveolar epithelial cell monolayers exposed to thrombin

2006 ◽  
Vol 290 (6) ◽  
pp. L1104-L1110 ◽  
Author(s):  
Xavier Trepat ◽  
Ferranda Puig ◽  
Nuria Gavara ◽  
Jeffrey J. Fredberg ◽  
Ramon Farre ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Epithelial Cell ◽  
Structural Integrity ◽  
Alveolar Epithelial Cell ◽  
Compressive Strain ◽  
Cell Junctions ◽  
Cell Monolayers ◽  
Alveolar Epithelial ◽  
Epithelial Cell Monolayers ◽  
Human Alveolar Epithelial Cell

Alveolar epithelial cells in patients with acute lung injury subjected to mechanical ventilation are exposed to increased procoagulant activity and mechanical strain. Thrombin induces epithelial cell stiffening, contraction, and cytoskeletal remodeling, potentially compromising the balance of forces at the alveolar epithelium during cell stretching. This balance can be further compromised by the loss of integrity of cell-cell junctions in the injured epithelium. The aim of this work was to study the effect of stretch on the structural integrity and micromechanics of human alveolar epithelial cell monolayers exposed to thrombin. Confluent and subconfluent cells (A549) were cultured on collagen-coated elastic substrates. After exposure to thrombin (0.5 U/ml), a stepwise cell stretch (20%) was applied with a vacuum-driven system mounted on an inverted microscope. The structural integrity of the cell monolayers was assessed by comparing intercellular and intracellular strains within the monolayer. Strain was measured by tracking beads tightly bound to the cell surface. Simultaneously, cell viscoelasticity was measured using optical magnetic twisting cytometry. In confluent cells, thrombin did not induce significant changes in transmission of strain from the substrate to overlying cells. By contrast, thrombin dramatically impaired the ability of subconfluent cells to follow imposed substrate deformation. Upon substrate unstretching, thrombin-treated subconfluent cells exhibited compressive strain (9%). Stretch increased stiffness (56–62%) and decreased cell hysteresivity (13–22%) of vehicle cells. By contrast, stretch did not increase stiffness of thrombin-treated cells, suggesting disruption of cytoskeletal structures. Our findings suggest that thrombin could exacerbate epithelial barrier dysfunction in injured lungs subjected to mechanical ventilation.

Human Alveolar Epithelial Cell Responses to Core–Shell Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

Langmuir ◽  
2015 ◽  
Vol 31 (13) ◽  
pp. 3829-3839 ◽  
Author(s):  
Doris Antoinette Mbeh ◽  
Laura Karina Mireles ◽  
Dimitri Stanicki ◽  
Lyes Tabet ◽  
Karim Maghni ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Epithelial Cell ◽  
Iron Oxide Nanoparticles ◽  
Alveolar Epithelial Cell ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Core Shell ◽  
Alveolar Epithelial ◽  
Cell Responses ◽  
Human Alveolar Epithelial Cell
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