Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production

2019 ◽  
Vol 127 (4) ◽  
pp. 430-440 ◽  
Author(s):  
Yung-Yu Tsai ◽  
Takao Ohashi ◽  
Chih-Chan Wu ◽  
Dolgormaa Bataa ◽  
Ryo Misaki ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Lipid Production ◽  
Fatty Acid Desaturase ◽  
Rhodosporidium Toruloides ◽  
Oleic Acid Content ◽  
Yeast Lipid

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

scholarly journals Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene

2020 ◽  
Author(s):  
Nan Wu ◽  
Long Jiang ◽  
Xiaoming Yu ◽  
Xiangbo Yang ◽  
Dianyuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Grain Development ◽  
Expression Levels ◽  
Soybean Fatty Acid ◽  
Structural Differences

Abstract Background To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.Results Database analysis, phylogenetic tree analysis, transmembrane structure prediction, amino acid sequence alignment, intron exon structure analysis, and protein motif analysis were performed for seven genes in the FAD2 family. The results showed that GmFAD2-1 and GmFAD2-2b and Other genes are structurally different, and it is speculated that their functions also have weak changes. By analyzing gene expression patterns at different stages of soybean grain development, the expression levels of the family genes at different stages of grain development first increase, then decrease, and finally decrease. For stability, the correlation analysis showed that the gene expression was negatively correlated with oleic acid content, and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.Conclusions Bioinformatics and expression analysis of genes in the FAD2 family. Identify the structural differences of each gene, and the differences in expression levels at different stages of soybean variety and grain development, and initially identify the differences in function size and catalytic efficiency between genes, in order to create new high-oleic soybean varieties using genetic engineering Quality, providing a theoretical basis.

scholarly journals Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene

2020 ◽  
Author(s):  
Nan Wu ◽  
Long Jiang ◽  
Xiaoming Yu ◽  
Xiangbo Yang ◽  
Dianyuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Grain Development ◽  
Expression Levels ◽  
Soybean Fatty Acid ◽  
Structural Differences

Abstract Background To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.Results Database analysis, phylogenetic tree analysis, transmembrane structure prediction, amino acid sequence alignment, intron exon structure analysis, and protein motif analysis were performed for seven genes in the FAD2 family. The results showed that GmFAD2-1 and GmFAD2-2b and Other genes are structurally different, and it is speculated that their functions also have weak changes. By analyzing gene expression patterns at different stages of soybean grain development, the expression levels of the family genes at different stages of grain development first increase, then decrease, and finally decrease. For stability, the correlation analysis showed that the gene expression was negatively correlated with oleic acid content, and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.Conclusions Bioinformatics and expression analysis of genes in the FAD2 family. Identify the structural differences of each gene, and the differences in expression levels at different stages of soybean variety and grain development, and initially identify the differences in function size and catalytic efficiency between genes, in order to create new high-oleic soybean varieties using genetic engineering Quality, providing a theoretical basis.

Enhanced Oleic Acid Content in the Soybean Mutant M23 Is Associated with the Deletion in the Fad2-1a Gene Encoding a Fatty Acid Desaturase

2007 ◽  
Vol 84 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Devinder Sandhu ◽  
Jessie L. Alt ◽  
Curtis W. Scherder ◽  
Walter R. Fehr ◽  
Madan K. Bhattacharyya
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Gene Encoding

scholarly journals Construction and Analysis of GmFAD2-1A and GmFAD2-2A Soybean Fatty Acid Desaturase Mutants Based on CRISPR/Cas9 Technology

2020 ◽  
Vol 21 (3) ◽  
pp. 1104 ◽  
Author(s):  
Nan Wu ◽  
Qiang Lu ◽  
Piwu Wang ◽  
Qi Zhang ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Protein Content ◽  
Acid Content ◽  
Double Mutant ◽  
Fatty Acid Desaturase ◽  
Linoleic Acid Content ◽  
Oleic Acid Content ◽  
Editing Efficiency

The soybean fatty acid desaturase family is composed of seven genes, but the function of each gene has not been reported. Bioinformatics was used to analyse the structure of genes in this family, as well as the correlation between Δ12-fatty acid desaturase II (FAD2) expression and oleic acid content on different days after flowering of soybean. In the present study, CRISPR/Cas9 technology was used to construct single and double mutant knockout vectors of functional genes in the FAD2 family. Analysis of the molecular biology and expression patterns of genes in the FAD2 family, namely, GmFAD2-1A (Glyma.10G278000) and GmFAD2-2A (Glyma.19G147300), showed that they had little homology with other soybean FAD2 genes, and that their function was slightly changed. Sequencing of the target showed that the editing efficiency of the GmFAD2-1A and GmFAD2-2A genes was 95% and 55.56%, respectively, and that the double mutant editing efficiency was 66.67%. The mutations were divided into two main types, as follows: base deletion and insertion. A near-infrared grain analyser determined the following results: In the T2 generation, the oleic acid content increased from 17.10% to 73.50%; the linoleic acid content decreased from 62.91% to 12.23%; the protein content increased from 37.69% to 41.16%; in the T3 generation, the oleic acid content increased from 19.15% to 72.02%; the linoleic acid content decreased from 56.58% to 17.27%. In addition, the protein content increased from 37.52% to 40.58% compared to that of the JN38 control variety.

Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

scholarly journals Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 222 ◽  
Author(s):  
Qinfu Sun ◽  
Jueyi Xue ◽  
Li Lin ◽  
Dongxiao Liu ◽  
Jian Wu ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Transcription Factors ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Content ◽  
Fatty Acid Biosynthesis ◽  
Oleic Acid Content ◽  
Acid Biosynthesis ◽  
Brassica Napus L

Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

Environmental effects on seed composition of Victorian canola

2000 ◽  
Vol 40 (5) ◽  
pp. 679 ◽  
Author(s):  
F. M. Pritchard ◽  
H. A. Eagles ◽  
R. M. Norton ◽  
P. A. Salisbury ◽  
M. Nicolas
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Environmental Effects ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Linoleic Acid Content ◽  
Glucosinolate Content ◽  
Oleic Acid Content ◽  
North Eastern ◽  
Spring Temperatures

Data from advanced breeding experiments between 1985 and 1994 were used to determine the effects of region, year and environment on the quality of canola grown across Victoria. Estimates from these unbalanced data were made using residual maximum likelihood. Environmental effects were large relative to cultivar effects for oil and protein content, while the reverse occurred for glucosinolate content. High oil contents (and low seed protein contents) were correlated with cooler spring temperatures and higher spring rainfall. Oil contents were lowest, on average, in canola grown in dry years, or from the hotter regions, such as the Mallee, and were highest in canola from the cooler, wetter regions, such as south-western and north-eastern Victoria. Fatty acid composition varied with year and region. Means for saturated fatty acid content averaged 6.4 0.1%. The oleic acid content averaged 60.3 0.4% and was higher in canola grown in central Victoria and the Wimmera, and in most years, in north-eastern Victoria compared with other regions. Low temperatures and low rainfall reduced oleic acid content. Linoleic acid content averaged 19.7 0.3% and linolenic acid averaged 10.4 0.3%, with the content of these fatty acids negatively correlated with the content of oleic acid. Erucic acid levels were below 0.6% in all regions.

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