Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene
Abstract Background To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.Results Database analysis, phylogenetic tree analysis, transmembrane structure prediction, amino acid sequence alignment, intron exon structure analysis, and protein motif analysis were performed for seven genes in the FAD2 family. The results showed that GmFAD2-1 and GmFAD2-2b and Other genes are structurally different, and it is speculated that their functions also have weak changes. By analyzing gene expression patterns at different stages of soybean grain development, the expression levels of the family genes at different stages of grain development first increase, then decrease, and finally decrease. For stability, the correlation analysis showed that the gene expression was negatively correlated with oleic acid content, and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.Conclusions Bioinformatics and expression analysis of genes in the FAD2 family. Identify the structural differences of each gene, and the differences in expression levels at different stages of soybean variety and grain development, and initially identify the differences in function size and catalytic efficiency between genes, in order to create new high-oleic soybean varieties using genetic engineering Quality, providing a theoretical basis.