Internal radial perfusion bioreactor promotes decellularized and recellularization of rat uterine tissue

SummaryThree types of plasminogen activator could be distinguished in extracts from human uterine tissue. The activators differed in thermostability or in mode of inhibition by EACA.All the extracts contained stable as well as labile activators. The saline extracts were uniformly inhibited by increasing concentrations of EACA. Extracts made with 2 M ammonium thiocyanate were either uniformly inhibited by EACA or showed deflections indicating contamination with an activator, which was inhibited in a biphasic manner. It was possible to distinguish between: (1) An activator, abundantly present in the tissue, which was uniformly inhibited and stable. (2) Another uniformly inhibited activator, which was labile. (3) An activator, inhibited in a biphasic manner, similar to urokinase, which was present in varying amounts in uteri with the endometrium in the proliferative phase.Gel filtration of the uterine extracts showed two major activity peaks corresponding to particle sizes of 60,000 dalton and about 10,000 dalton.Antiserum to purified plasminogen activator, prepared from porcine ovaries, inhibited the activity of the human uterine extracts, but not the activities of human urokinase or urine. Urokinase antiserum in a concentration completely inhibiting human urine or urokinase, inhibited only 10% or less of the activities of human uterine extracts.

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Behaviour and Quantitation of Extrinsic (Tissue-Type) Plasminogen Activator in Human Blood

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665244 ◽

1983 ◽

Vol 50 (02) ◽

pp. 518-523 ◽

Cited By ~ 16

Author(s):

C Kluft ◽

A F H Jie ◽

R A Allen

Keyword(s):

Plasminogen Activator ◽

Venous Occlusion ◽

Tissue Type ◽

Functional Assay ◽

Uterine Tissue ◽

Tissue Type Plasminogen Activator ◽

Type Plasminogen Activator ◽

Baseline Values

SummaryFunctional assay of extrinsic (tissue-type) plasminogen activator (EPA) in plasma on fibrin plates was evaluated. Using specific quenching antibodies, we demonstrated the method to be specific for EPA under all conditions tested. Contributions of urokinases and intrinsic activators were excluded. The quantity of EPA in blood samples, as compared with purified uterine tissue activator, shows 1 blood activator unit (BAU) to be comparable to 0.93 ng.The median values for EPA activity for healthy volunteers were: baseline, 1.9 BAU/ml (n = 123); diurnal, 5.5 BAU/ml (n = 12); DDAVP administration, 11.7 BAU/ml (n = 39); exhaustive exercise, 25 BAU/ml (n = 24); venous occlusion (15 min), 35 BAU/ml (n = 61). A large inter-individual variation in EPA activity was found, while individual baseline values tended to be constant for periods of weeks.In vitro in blood EPA activity shows a disappearance of 50% in about 90 min at 37° C; EPA activity in euglobulin fractions is stable for ≤2 hr at 37° C.A rapid decrease in EPA activity occurs in vivo, as noted after extracorporal circulation and exercise stimulation (t½ decay, 2-5 min).

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ÜBER DIE BINDUNG VON OESTRON AN ÜBERLEBENDES UTERUSGEWEBE DER RATTE

Acta Endocrinologica ◽

10.1530/acta.0.0360520 ◽

1961 ◽

Vol 36 (4) ◽

pp. 520-526

Author(s):

Wilhelm Dirscherl ◽

Herbert Schriefers

Keyword(s):

Incubation Medium ◽

Uterine Tissue

ABSTRACT Surviving rat uterine tissue is capable of taking up oestrone from the incubation medium. Experiments to liberate the hormone have shown, that there exist different binding strengths.

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16α-HYDROXYLASE ACTIVITY IN UTERINE TISSUE OF THE RHESUS MONKEY

Acta Endocrinologica ◽

10.1530/acta.0.072s004 ◽

1973 ◽

Vol 71 (4_Suppl) ◽

pp. S4 ◽

Cited By ~ 1

Author(s):

M. Breckwoldt ◽

A. Trautwein ◽

B. P. Lisboa

Keyword(s):

Rhesus Monkey ◽

Uterine Tissue ◽

Hydroxylase Activity

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HEAT TRANSFER ANALYSIS FOR A PERFUSION BIOREACTOR

10.26678/abcm.enebi2018.eeb18-0220 ◽

2018 ◽

Author(s):

Júlia Nogueira ◽

daniel kemmoku ◽

Pedro Noritomi ◽

Rodrigo Rezende ◽

Janaina Dernowsek ◽

...

Keyword(s):

Heat Transfer ◽

Heat Transfer Analysis ◽

Perfusion Bioreactor

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The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.01.1013 ◽

2019 ◽

Vol 20 (1) ◽

pp. 75-84

Keyword(s):

Early Pregnancy ◽

Histopathological Examination ◽

Early Period ◽

Interleukin 10 ◽

Treated Group ◽

Female Rats ◽

Male Rats ◽

Mrna Levels ◽

Uterine Tissue ◽

Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

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Preconditioning Human Adipose-Derived Stromal Cells on Decellularized Adipose Tissue Scaffolds within a Perfusion Bioreactor Modulates Cell Phenotype and Promotes a Pro-Regenerative Host Response

SSRN Electronic Journal ◽

10.2139/ssrn.3732500 ◽

2020 ◽

Author(s):

Tim T.Y. Han ◽

John T. Walker ◽

Aaron Grant ◽

Gregory A. Dekaban ◽

Lauren Elizabeth Flynn

Keyword(s):

Adipose Tissue ◽

Stromal Cells ◽

Host Response ◽

Tissue Scaffolds ◽

Cell Phenotype ◽

Perfusion Bioreactor ◽

Adipose Derived Stromal Cells

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Building a stem cell-based primate uterus

Communications Biology ◽

10.1038/s42003-021-02233-8 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Sophie Bergmann ◽

Magdalena Schindler ◽

Clara Munger ◽

Christopher A. Penfold ◽

Thorsten E. Boroviak

Keyword(s):

Stem Cell ◽

Fetal Development ◽

Embryo Implantation ◽

In Vitro Models ◽

Uterine Tissue ◽

Controlled Assembly ◽

Uterine Cancers ◽

Term Births ◽

Key Questions

AbstractThe uterus is the organ for embryo implantation and fetal development. Most current models of the uterus are centred around capturing its function during later stages of pregnancy to increase the survival in pre-term births. However, in vitro models focusing on the uterine tissue itself would allow modelling of pathologies including endometriosis and uterine cancers, and open new avenues to investigate embryo implantation and human development. Motivated by these key questions, we discuss how stem cell-based uteri may be engineered from constituent cell parts, either as advanced self-organising cultures, or by controlled assembly through microfluidic and print-based technologies.

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