Capillary electrophoresis-based online immobilized enzyme reactor for beta-glucosidase kinetics assays and inhibitors screening

2019 ◽  
Vol 1110-1111 ◽  
pp. 67-73 ◽  
Author(s):  
Zhao-Yu Wu ◽  
Hao Zhang ◽  
Qiao-Qiao Li ◽  
Feng-Qing Yang ◽  
De-Qiang Li
Keyword(s):  
Capillary Electrophoresis ◽  
Immobilized Enzyme ◽  
Enzyme Reactor ◽  
Immobilized Enzyme Reactor ◽  
Beta Glucosidase

A capillary electrophoresis-based immobilized enzyme reactor using graphene oxide as a support via layer by layer electrostatic assembly

The Analyst ◽  
2014 ◽  
Vol 139 (8) ◽  
pp. 1973-1979 ◽  
Author(s):  
Zhengri Yin ◽  
Wenwen Zhao ◽  
Miaomiao Tian ◽  
Qian Zhang ◽  
Liping Guo ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Graphene Oxide ◽  
Immobilized Enzyme ◽  
Layer By Layer ◽  
Enzyme Reactor ◽  
Immobilized Enzyme Reactor ◽  
Electrostatic Assembly ◽  
On Line ◽  
Line Analysis

Using graphene oxide as an enzyme support, we developed a novel CE-based microreactor via layer-by-layer electrostatic assembly, which can be used for accurate on-line analysis and characterization of peptides and proteins.

scholarly journals Aldehyde oxidase assay by capillary electrophoresis: From off‐line, online up to immobilized enzyme reactor

2020 ◽  
Vol 43 (17) ◽  
pp. 3565-3572
Author(s):  
Shengyun Huang ◽  
Andrea Celá ◽  
Erwin Adams ◽  
Zdenĕk Glatz ◽  
Ann Van Schepdael
Keyword(s):  
Capillary Electrophoresis ◽  
Immobilized Enzyme ◽  
Aldehyde Oxidase ◽  
Enzyme Reactor ◽  
Immobilized Enzyme Reactor

Capillary electrophoresis-based immobilized enzyme reactor using particle-packing technique

2014 ◽  
Vol 1352 ◽  
pp. 80-86 ◽  
Author(s):  
Lina Liu ◽  
Bo Zhang ◽  
Qian Zhang ◽  
Yanhong Shi ◽  
Liping Guo ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Immobilized Enzyme ◽  
Particle Packing ◽  
Enzyme Reactor ◽  
Immobilized Enzyme Reactor

scholarly journals Study of the geometry of open channels in a layer-bed-type microfluidic immobilized enzyme reactor

2021 ◽  
Author(s):  
Cynthia Nagy ◽  
Robert Huszank ◽  
Attila Gaspar
Keyword(s):  
Immobilized Enzyme ◽  
High Surface ◽  
Volume Ratio ◽  
Volumetric Flow Rate ◽  
Enzyme Reactor ◽  
Channel Pattern ◽  
Immobilized Enzyme Reactor ◽  
Split Flow ◽  
Parallel Streams ◽  
Enzymatic Microreactors

AbstractThis paper aims at studying open channel geometries in a layer-bed-type immobilized enzyme reactor with computer-aided simulations. The main properties of these reactors are their simple channel pattern, simple immobilization procedure, regenerability, and disposability; all these features make these devices one of the simplest yet efficient enzymatic microreactors. The high surface-to-volume ratio of the reactor was achieved using narrow (25–75 μm wide) channels. The simulation demonstrated that curves support the mixing of solutions in the channel even in strong laminar flow conditions; thus, it is worth including several curves in the channel system. In the three different designs of microreactor proposed, the lengths of the channels were identical, but in two reactors, the liquid flow was split to 8 or 32 parallel streams at the inlet of the reactor. Despite their overall higher volumetric flow rate, the split-flow structures are advantageous due to the increased contact time. Saliva samples were used to test the efficiencies of the digestions in the microreactors. Graphical abstract

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