Antiseptic negative pressure instillation therapy for the treatment of septic wound healing deficits in oral and maxillofacial surgery

As a source of growth factors for expediting wound healing and tissue regeneration, plasma-rich plasma (PRP) has been extensively applied in diverse fields including orthopaedics, ophthalmology, oral and maxillofacial surgery, dentistry, and gynaecology. However, the function of PRP in metabolic regulations remains enigmatic. A standardized method was devised herein to enrich growth factors and to lyophilize it as enhanced PRP (ePRP) powder, which could become ubiquitously available without mechanical centrifugation in clinical practice. To identify metabolic reprogramming in human dermal fibroblasts under ePRP treatment, putative metabolic targets were identified by transcriptome profiling and validated for their metabolic effects and mechanism. ePRP does not only promote wound healing but re-aligns energy metabolism by shifting to glycolysis through stimulation of glycolytic enzyme activity in fibroblasts. On the contrary, oxygen consumption rates and several mitochondrial respiration activities were attenuated in ePRP-treated fibroblasts. Furthermore, ePRP treatment drives the mitochondrial resetting by hindering the mitochondrial biogenesis-related genes and results in a dampened mitochondrial mass. Antioxidant production was further increased by ePRP treatment to prevent reactive oxygen species formation. Besides, ePRP also halts the senescence progression of fibroblasts by activating SIRT1 expression. Importantly, the glycolytic inhibitor 2-DG can completely reverse the ePRP-enhanced wound healing capacity, whereas the mitochondrial inhibitor oligomycin cannot. This is the first study to utilize PRP for comprehensively investigating its effects on the metabolic reprogramming of fibroblasts. These findings indicate that PRP’s primary metabolic regulation is to promote metabolic reprogramming toward glycolytic energy metabolism in fibroblasts, preserving redox equilibrium and allowing anabolic pathways necessary for the healing and anti-ageing process.

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Influence of Trans-operative Complications on Socket Healing Following Dental Extractions

The Journal of Contemporary Dental Practice ◽

10.5005/jcdp-8-1-52 ◽

2007 ◽

Vol 8 (1) ◽

pp. 52-59 ◽

Cited By ~ 10

Author(s):

Wasiu Lanre Adeyemo ◽

Akinola Ladipo Ladeinde ◽

Mobolanle Olugbemiga Oguniewe

Keyword(s):

Wound Healing ◽

Alveolar Bone ◽

Bone Fractures ◽

Maxillofacial Surgery ◽

University Teaching ◽

Oral And Maxillofacial Surgery ◽

Extraction Site ◽

Operative Complications ◽

Socket Healing ◽

Healing Assessment

Abstract Aim Extraction healing complications have been attributed to several factors. The influence of trans-operative complications on an extraction site wound healing was the focus of this investigation. Methods and Materials This prospective study was conducted at the Oral Surgery Clinic of the Department of Oral and Maxillofacial Surgery of the Lagos University Teaching Hospital (LUTH) in Nigeria . Subjects selected were those referred for one or two adjacent extractions and who satisfied the inclusion criteria for the study. The relevant pre-operative information recorded for each patient were age and sex of patient, indications for extraction, time taken to extract the tooth, tooth/teeth removed, and any trans-operative complications. Extractions were performed with dental forceps, elevators, or both under local anaesthesia. Patients were blindly evaluated on the third and seventh post-operative day for socket healing assessment without reference to preoperative information on the patients. Results Seventy-three (24.25%) of 301 teeth considered for socket healing assessment had various transoperative complications due to accidental crown, root, or alveolar bone fractures. Of the 73 extractions with trans-operative complications during extraction, 18 developed a socket healing complication, while 17 of the 228 extractions without trans-operative complications developed socket healing complications (p = .000). The mean (SD) time taken to extract teeth developing healing complications was also found to be significantly longer than those without healing complications (p < .01). Conclusions The study demonstrated the combination of tooth/bone fragments in the socket and increased time of extraction due to trans-operative complications and accidents predispose to the development of extraction site wound healing disturbance. Citation Adeyemo WL, Ladeinde AL, Ogunlewe MO. Influence of Trans-operative Complications on Socket Healing Following Dental Extractions. J Contemp Dent Pract 2007 January;(8)1:052-059.

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Negative Pressure Pulmonary Edema After Oral and Maxillofacial Surgery

Anesthesia Progress ◽

10.2344/0003-3006-56.2.49 ◽

2009 ◽

Vol 56 (2) ◽

pp. 49-52 ◽

Cited By ~ 12

Author(s):

Hideki Mamiya ◽

Tatsuya Ichinohe ◽

Yuzuru Kaneko

Keyword(s):

Pulmonary Edema ◽

Negative Pressure ◽

Maxillofacial Surgery ◽

Upper Airway ◽

Intrathoracic Pressure ◽

Oral And Maxillofacial Surgery ◽

Verbal Command ◽

Plate Removal ◽

Negative Pressure Pulmonary Edema ◽

Jaw Deformity

Abstract Negative pressure pulmonary edema (NPPE) following upper airway obstruction (UAO) has been reported in several clinical situations. The main cause of NPPE is reported to be increased negative intrathoracic pressure. We present a case of NPPE that occurred after general anesthesia for plate removal after jaw deformity surgery. After completion of the surgery, administration of inhaled anesthetics was stopped and the patient opened his eyes on verbal command. Immediately after extubation, the patient stopped breathing and became cyanotic. Acute UAO following laryngospasm was suspected. Soon after reintubation, pink, frothy fluid came out of the endotracheal tube, and a tentative diagnosis of NPPE was made. Continuous positive airway pressure was applied. In addition, furosemide and dexamethasone were administered. By the next day, the symptoms had almost disappeared.

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The Effect of Black Cumin (Nigella sativa sp.) Gel Extract in Wound Healing Process Post Tooth Extraction

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.48.41 ◽

2020 ◽

Vol 48 ◽

pp. 41-46

Author(s):

Rahmi Syaflida Dalimunte ◽

Olivia Avriyanti Hanafiah ◽

Hendry Rusdy

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Tooth Extraction ◽

Nigella Sativa ◽

Healing Process ◽

Maxillofacial Surgery ◽

Control Group ◽

Wound Healing Process ◽

Oral And Maxillofacial Surgery ◽

Black Cumin

Objective: This study aims to investigate the effects of black cumin extract (Nigella sativa) on the process of wound healing in tooth extraction sockets. Material and Methods: This study involved 24 patients from the Department of Oral and Maxillofacial Surgery grouped into the control and treatment groups. After extracting the first or second molar left or right in adult patients, the black cumin extractwas applied to the socket in the treatment group as soon as possible while it was not applied in the control group. The sockets were divided into 1,2,3 groups, which were treated as black cumin extracts of 10%, 12%, and 14% respectively. On day 1,3,7, patients were recalled and sockets size were calculated to analyze wound healing. Results: Black cumin was found to be able to make the wound healing faster in the treatment group when compared to the control group. Although the effect was not immediately apparent on the second day, on the seventh day the group treated with black cumin extract had a smaller sizing average compared to the control group. The concentration of 12% black cumin extract was found to have the best effect on wound healing. Significance of findings: Black cumin extract can make wound healing in tooth extraction sockets in patients.

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A Double Blind Study on the Efficacy of Local Application of Hemocoagulase Solution in Wound Healing

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1334 ◽

2013 ◽

Vol 14 (3) ◽

pp. 394-400 ◽

Cited By ~ 2

Author(s):

Sachin Aslam ◽

PG Francis ◽

BHS Rao ◽

M Ummar ◽

JK Issac ◽

...

Keyword(s):

Wound Healing ◽

Epithelial Cells ◽

Maxillofacial Surgery ◽

Blind Study ◽

Local Application ◽

Dental Extraction ◽

Hemostatic Agent ◽

Oral And Maxillofacial Surgery ◽

Double Blind Study ◽

Double Blind

ABSTRACT Aim The present double blind study has been designed to evaluate the efficacy of local application of hemocoagulase solution as compared to a placebo in wound healing following dental extraction. Materials and methods A total of 20 patients who required dental extraction for orthodontic intervention were included. The hemocoagulase solution and a placebo were locally applied to the extraction sockets and the efficacy of the solution in terms of bleeding control, anti-inflammatory responses, its antiseptic properties and efficacy in wound healing were evaluated. Results The mean time required to achieve hemostasis was found to be 1.37 minutes in side A (test) and 2.33 minutes in side B (control) indicating that side A achieved faster hemostasis when compared to side B. At the 6th hour postoperatively, bleeding was not evident on either sides, and the amount of pain in side A was found to be less compared to side B. The number of RBCs, polymorphs, chronic inflammatory cells were not different in both the groups, whereas at 3rd postoperative day epithelial cells were greater in side A (test) compared to side B (control). Biopsy reports on the 12th postoperative day indicated that the number of fibroblasts, epithelial cells, collagen count was found to be greater in side A (test) compared to side B (control). Conclusion The topical hemocoagulase solution may be advocated in the field of oral and maxillofacial surgery, as a hemostatic agent and promoter of wound healing. However, further studies, with large number of cases and different clinical situations should be considered to authenticate the efficacy of this hemocoagulase solution in the practice of oral and maxillofacial surgery. Clinical significance Wound healing plays an important role in the success of any surgical procedure, such as extractions, and the hemocoagulase system may act as a hemostatic agent and a promoter of wound healing. How to cite this article Aslam S, Francis PG, Rao BHS, Ummar M, Issac JK, Nair RB. A Double Blind Study on the Efficacy of Local Application of Hemocoagulase Solution in Wound Healing. J Contemp Dent Pract 2013;14(3):394-400.

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A Review on the Application of Silver Nanoparticles in Oral and Maxillofacial Surgery

European Journal of Dentistry ◽

10.1055/s-0041-1731589 ◽

2021 ◽

Author(s):

Diane Isabel Selvido ◽

Bishwa Prakash Bhattarai ◽

Apiwat Riddhabhaya ◽

Kadkao Vongsawan ◽

Siripen Arunpraphan ◽

...

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Tissue Regeneration ◽

Maxillofacial Surgery ◽

Oral And Maxillofacial Surgery ◽

Clinical Settings ◽

Antibacterial Resistance ◽

Dental Setting ◽

Implant Dentistry ◽

Healing Bone

AbstractSilver nanoparticles (AgNPs) have been taken advantage of in dentistry because of their good antibacterial resistance and self-sustaining potential. However, in oral and maxillofacial surgery and implantology, there is a lesser amount of evidence. The few pieces of evidence need to be accentuated for possible amplification of its use in the dental setting. AgNPs in oral and maxillofacial surgery can be used in wound healing, bone healing, extractions, guided tissue regeneration, apical surgeries, oral cancer, and dental implants. This review aims to feature the utilization and application of AgNPs in oral and maxillofacial surgery and implant dentistry, emphasizing its need for potential future development in clinical settings.

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Evaluation of Early Wound Healing Scale in Oral and Maxillofacial Surgery

Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.joms.2019.06.052 ◽

2019 ◽

Vol 77 (9) ◽

pp. e30-e31

Author(s):

Y. Hamzani ◽

G. Chaushu ◽

B. Haj Yahya

Keyword(s):

Wound Healing ◽

Maxillofacial Surgery ◽

Oral And Maxillofacial Surgery ◽

Early Wound

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Fabrication of PANI-modified PVDF nanofibrous yarn for pH sensor

e-Polymers ◽

10.1515/epoly-2022-0013 ◽

2021 ◽

Vol 22 (1) ◽

pp. 69-74

Author(s):

Hongmei Zhao ◽

Zhang Dai ◽

Tian He ◽

Shufang Zhu ◽

Xu Yan ◽

...

Keyword(s):

Wound Healing ◽

Potential Application ◽

Polyvinylidene Fluoride ◽

Maxillofacial Surgery ◽

Ph Sensor ◽

Electrospinning Process ◽

Oral And Maxillofacial Surgery ◽

Nanofibrous Yarn ◽

Ph Range

Abstract In recent years, with the rise of an intelligent concept, oral and maxillofacial surgery smart dressing had also attracted the interest of researchers, especially for the pH sensor with flexible medium. In this study, polyvinylidene fluoride (PVDF) nanofibrous yarn was fabricated by a conjugate electrospinning process and modified with in situ polymerization of polyaniline (PANI) forming a PANI/PVDF yarn. By a weaving process, these yarns could be weaved into a fabric. It was found that both the PANI/PVDF yarn and the fabric showed a sensitivity to pH, about −48.53 mV per pH for yarn and −38.4 mVper pH for fabric, respectively, in the pH range of 4.0–8.0. These results indicated that the prepared PANI-modified PVDF yarn and fabric might have a potential application in intelligent oral and maxillofacial surgery dressings for monitoring wound healing.

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Using Negative Pressure Therapy Can Accelerate Wound Healing

Internal Medicine News ◽

10.1016/s1097-8690(05)71480-3 ◽

2005 ◽

Vol 38 (3) ◽

pp. 43

Author(s):

Kerri Wachter

Keyword(s):

Wound Healing ◽

Negative Pressure ◽

Negative Pressure Therapy ◽

Pressure Therapy ◽

Accelerate Wound Healing

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Experience of negative pressure wound therapy over sternal wound healing: A retrospective review

WCET Journal ◽

10.33235/wcet.39.2.9-18 ◽

2019 ◽

Vol 39 (2) ◽

pp. 9-18

Author(s):

Wai Sze Ho ◽

Wai Kuen Lee ◽

Ka Kay Chan ◽

Choi Ching Fong

Keyword(s):

Wound Healing ◽

Negative Pressure ◽

Negative Pressure Wound Therapy ◽

Assessment Tool ◽

Wound Care ◽

Wound Therapy ◽

Treatment Pathways ◽

Wound Assessment ◽

One Year

Objectives The aim of this study was to retrospectively review the effectiveness of negative pressure wound therapy (NPWT) in sternal wound healing with the use of the validated Bates-Jensen Wound Assessment Tool (BWAT), and explore the role of NPWT over sternal wounds and future treatment pathways. Methods Data was gathered from patients' medical records and the institution's database clinical management system. Seventeen subjects, who had undergone cardiothoracic surgeries and subsequently consulted the wound care team in one year were reviewed. Fourteen of them were included in the analysis. Healing improvement of each sternal wound under continuous NPWT and continuous conventional dressings was studied. In total, 23 continuous NPWT and 13 conventional dressing episodes were analysed with the BWAT. Results Among conventional dressing episodes, sternal wound improvement was 2.5–3% over 10 days to 3.5 weeks, whereas 4–5% sternal healing was achieved in 5 days to 2 weeks with sternal wire presence. Better healing at 11% in 1 week by conventional dressing was attained after sternal wire removal. In NPWT episodes, 8–29%, 13–24%, and 15–46% of healing was observed in 2 weeks, 3.5 to 5 weeks and 6 to 7 weeks, respectively. Only 39% wound healing was acquired at the 13th week of NPWT in one subject. With sternal wire present, 6%–29% wound healing progress was achieved by NPWT in 1–4 weeks, and 16–23% wound improvement in 2 to 4.5 weeks by NWPT after further surgical debridement. After sternal wire removal, 6–34% sternal wound healing occurred by continuous NPWT for 1–2 weeks, and maximum healing at 46% after 2.5 weeks of NPWT were observed. Conclusions Better wound healing was achieved in the NPWT group in comparison to conventional dressings alone. However, suboptimal sternal wound healing by NPWT alone was observed. Removal of sternal wire may improve the effectiveness of NPWT. Successful tertiary closure after NPWT among subjects supports the important bridging role of NPWT in sternal wound healing. Factors causing stagnant sternal wound healing by NPWT alone are discussed.

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