Unsaturated fatty acid-tuned assembly of photosensitizers for enhanced photodynamic therapy via lipid peroxidation

2013 ◽

Vol 726-731 ◽

pp. 4241-4247

Author(s):

Wei Zhang ◽

Jin Zhong Xie

Keyword(s):

Lipid Peroxidation ◽

Fatty Acid ◽

Low Temperature ◽

Membrane Permeability ◽

Unsaturated Fatty Acid ◽

Soluble Protein ◽

Soluble Sugar ◽

Membrane Lipid ◽

Cold Hardening ◽

Membrane Lipid Peroxidation

An indoor low temperature experiment was conducted to study the variations of membrane permeability, malondialdehyde, soluble protein and soluble sugar contents, superoxide dismutase (SOD) and peroxidase (POD) activities and membrane lipid fatty acid content in leaves and roots of sympodial bamboo seedlings (Dendrocalamus latiflorus)under different levels of cold stress. Results showed that after low temperature pretreatment (8 °C) for 15 days, the soluble protein, soluble sugar contents and POD activities in leaves as well as soluble sugar contents and POD activities in roots were increased obviously. After cold-hardening (-2 °C) for 72 h, the soluble protein, soluble sugar contents and SOD, POD activities in leaves and SOD, POD activities and ratio of membrane lipid unsaturated fatty acid in roots with pretreatment were obviously higher than those without pretreatment. Membrane permeability in leaves with pretreatment was obviously lower than that without pretreatment. But the level of membrane lipid peroxidation in leaves with pretreatment was significantly greater than that before cold-hardening. While the level of membrane permeability and lipid peroxidation in roots with pretreatment had no significant change after cold-hardening. Overall, leaves had higher soluble protein, soluble sugar contents and POD activities to avoid low temperature injuries while roots had higher SOD, POD activities and unsaturated fatty acid to avoid membrane lipid peroxidation and membrane injuries.

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Disaccahrides-Based Cryo-Formulant Effect on Modulating Phospho/Mitochondrial Lipids and Biological Profiles of Human Leukaemia Cells

Cellular Physiology and Biochemistry ◽

10.33594/000000360 ◽

2021 ◽

Vol 55 (2) ◽

pp. 206-221

Keyword(s):

Lipid Peroxidation ◽

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Saturated Fatty Acids ◽

Acid Oxidation ◽

Human Leukemia ◽

Oxygen Scavenger ◽

Mitochondrial Inner Membrane ◽

Cell Based Therapy ◽

Biological Patterns

Background/Aims: The use of novel cryo-additive agents to increase cell viability post-cryopreservation is paramount to improve future cell based-therapy treatments. We aimed to establish the Human Leukemia (HL-60) cells lipidomic and biological patterns when cryo-preserved in DMSO alone and with 300 µM Nigerose (Nig), 200 µM Salidroside (Sal) or a combination of Nig (150 µM) and Sal (100 µM). Methods: HL-60 cells were pre-incubated with Nig/Sal prior, during and post cryopreservation, and subjected to global lipidomic analysis. Malondialdeyhde (MDA), released lactate dehydrogenase (LDH) and reactive oxygen scavenger (ROS) measurements were also carried out to evaluate levels of lipid peroxidation and cytotoxicity. Results: Cryopreserving HL-60 cells in DMSO with Nig and Sal provided optimal protection against unsaturated fatty acid oxidation. Post-thaw, cellular phospholipids and mitochondrial cardiolipins were increased by Nig/Sal as the ratio of unsaturated to saturated fatty acids 2.08 +/- 0.03 and 0.95 +/- 0.09 folds respectively in comparison to cells cryopreserved in DMSO alone (0.49 +/- 0.05 and 0.86 +/- 0.10 folds). HL-60 lipid peroxidation levels in the presence of DMSO + Nig and Sal combined were significantly reduced relative to pre-cryopreservation levels (10.91 +/- 2.13 nmole) compared to DMSO (17.1 +/- 3.96 nmole). DMSO + Nig/Sal combined also significantly reduced cell cytotoxicity post-thaw (0.0128 +/- 0.00182 mU/mL) in comparison to DMSO (0.0164 +/- 0.00126 mU/mL). The combination of Nig/Sal also reduced significantly ROS levels to the levels of prior cryopreservation of HL-60. Conclusion: Overall, the establishment of the cryopreserved HL-60 cells lipidomic and the corresponding biological profiles showed an improved cryo-formulation in the presence of DMSO with the Nig/Sal combination by protecting the, mitochondrial inner membrane, unsaturated fatty acid components (i. e. Cardiolipins) and total phospholipids.

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IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/57/6/13711 ◽

2019 ◽

Vol 57 (6) ◽

pp. 665 ◽

Cited By ~ 1

Author(s):

Yen Thi Hoang ◽

Quynh Thi Thu Tran ◽

Ha Hoang Chu ◽

Tuyen Thi Do ◽

Thanh Tat Dang ◽

...

Keyword(s):

Fatty Acid ◽

Unsaturated Fatty Acid ◽

Unsaturated Fatty Acids ◽

Nitrogen Sources ◽

16S Rrna Analysis ◽

Rhodovulum Sulfidophilum ◽

Purple Nonsulfur Bacterium ◽

High Lipid ◽

Physiological Properties ◽

Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

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TRIGLYCERIDE COMPOSITION OF SIXTEEN STRAINS OF MARINE DIATOM

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v5i1.7762 ◽

2013 ◽

Vol 5 (1) ◽

Author(s):

Lily M.G. Panggabean ◽

Abdullah Rasyid ◽

Zarrah Duniani ◽

Yana Meliana ◽

Indah Kurniasih

Keyword(s):

Mass Spectrometry ◽

Fatty Acid ◽

Double Bond ◽

Unsaturated Fatty Acid ◽

Ion Trap ◽

Carbon Chain ◽

Highly Unsaturated Fatty Acid ◽

Marine Diatom ◽

Ion Trap Mass Spectrometry ◽

Spectra Analysis

Trigliceride or triacylglicerol (TAG) composition in crude oil of sixteen strain of marine diatom has been detected by spectra analyses on an Electrospray - Ion Trap – Mass Spectrometry (ESI-IT-MS) HCT Bruker-Daltonic GmbH instrument with AgNO3 used as coordination ionization agent. Biomass samples of each microalga strain were taken from early and late stationary cultures in f/2 enriched seawater and algal oils were extracted according to Bligh and Dyer. Results from spectra analysis showed that P-Pt-P (C16:0-C16:1-C16:0) were distinguished in TAG from diatom strains Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.1, Thalasiossira sp.2, Thalasiossira sp.3, Navicula sp. 1, Navicula sp. 2, Navicula sp. 3, Navicula sp. 4, Nitzschia sp. 2 and Amphora sp. In contrast, TAGs in Melosira sp. included P-P-P (C16:0-C16:0-C16:0) and P-P-O (C16:0-C16:0-C18:1) were identified. TAGs from Chaetoceros sp. were the most varies among samples, i.e. P-Pt-P (C16:0-C16:1-C16:0), A-P-M (C20:4-C16:0-C14:0), P-Pt-Lt (C16:0-C16:1-C18:3), P-Pt-A (C16:0-C16:1-C20:4), D-P-P (C22:6-C16:0-C16:0), A-Ln-P (C20:4-C18:2-C16:0). Various TAGs were also detected in Nitzschia sp.2, i.e. P-Pt-M (C16:0-C16:1-C14:0), P-Pt-P (C16:0-C16:1-C16:0), P-Pt-S (C16:0-C16:1-C18:0), P-Pt-A (C16:0-C16:1-C20:4). TAGs composition in Skeletonema strains that similar to those in Nitzschia sp.1 has longer carbon, i.e. P-P-O (C16:0-C16:0-C18:1), P-O-O (C16:0-C18:1-C18:1) and O-O-O (C18:1-C18:1-C18:1). TAGs with longer carbon chain and more double bond including highly unsaturated fatty acid C20:4 were increased with culture age in diatoms Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.2, Navicula sp.1 and Nitzschia sp. 2.Keywords: diatom, TAG, ESI-IT-MS, f/2, early and late stationary

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