Thermophilic anaerobic digestion of olive mill wastewater in an upflow packed bed reactor: Evaluation of 16S rRNA amplicon sequencing for microbial analysis

Little information on poly(l-lactic acid) (PLLA) treatment-associated microbiota in thermophilic anaerobic digestion reactors is available. Here, we provide 16S rRNA gene sequence data on microbiota in a thermophilic anaerobic digestion reactor converting PLLA to methane for 336 days. Data comprising 99,566 total high-quality reads were tabulated at the taxonomic class level.

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Biodegradation of olive mill wastewater phenolic compounds in a thermophilic anaerobic upflow packed bed reactor and assessment of their toxicity in digester effluents

Journal of Environmental Management ◽

10.1016/j.jenvman.2019.109882 ◽

2020 ◽

Vol 255 ◽

pp. 109882 ◽

Cited By ~ 5

Author(s):

C. Genethliou ◽

M. Kornaros ◽

S. Dailianis

Keyword(s):

Phenolic Compounds ◽

Packed Bed ◽

Olive Mill Wastewater ◽

Packed Bed Reactor ◽

Olive Mill

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Packed-bed biofilm reactor for semi-continuous anaerobic digestion of olive mill wastewater: performances and COD mass balance analysis

Environmental Technology ◽

10.1080/09593330.2019.1578830 ◽

2019 ◽

Vol 41 (20) ◽

pp. 2657-2669 ◽

Cited By ~ 5

Author(s):

Ghizlane Enaime ◽

Abdelaziz Baçaoui ◽

Abdelrani Yaacoubi ◽

Stephan Berzio ◽

Marc Wichern ◽

...

Keyword(s):

Anaerobic Digestion ◽

Mass Balance ◽

Packed Bed ◽

Olive Mill Wastewater ◽

Biofilm Reactor ◽

Balance Analysis ◽

Mass Balance Analysis ◽

Olive Mill

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Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion

PLoS ONE ◽

10.1371/journal.pone.0257651 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0257651

Author(s):

Riku Sakurai ◽

Shuhei Takizawa ◽

Yasuhiro Fukuda ◽

Chika Tada

Keyword(s):

Anaerobic Digestion ◽

16S Rrna ◽

Loading Rate ◽

Amplicon Sequencing ◽

Long Chain Fatty Acids ◽

Loading Rates ◽

16S Rrna Amplicon Sequencing ◽

Copy Numbers ◽

Hydrogenotrophic Methanogens ◽

Abundant Genus

Scum is formed by the adsorption of long-chain fatty acids (LCFAs) onto biomass surface in anaerobic digestion of oily substrates. Since scum is a recalcitrant substrate to be digested, it is disposed via landfilling or incineration, which results in biomass washout and a decrease in methane yield. The microbes contributing to scum degradation are unclear. This study aimed to investigate the cardinal microorganisms in anaerobic scum digestion. We pre-incubated a sludge with scum to enrich scum-degrading microbes. Using this sludge, a 1.3-times higher methane conversion rate (73%) and a faster LCFA degradation compared with control sludge were attained. Then, we analyzed the cardinal scum-degrading microbes in this pre-incubated sludge by changing the initial scum-loading rates. Increased 16S rRNA copy numbers for the syntrophic fatty-acid degrader Syntrophomonas and hydrogenotrophic methanogens were observed in scum high-loaded samples. 16S rRNA amplicon sequencing indicated that Syntrophomonas was the most abundant genus in all the samples. The amino-acid degrader Aminobacterium and hydrolytic genera such as Defluviitoga and Sporanaerobacter became more dominant as the scum-loading rate increased. Moreover, phylogenic analysis on Syntrophomonas revealed that Syntrophomonas palmitatica, which is capable of degrading LCFAs, related species became more dominant as the scum-loading rate increased. These results indicate that a variety of microorganisms that degrade LCFAs, proteins, and sugars are involved in effective scum degradation.

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Development of a high-rate anaerobic thermophilic upflow packed bed reactor for efficient bioconversion of diluted three-phase olive mill wastewater into methane

Fuel ◽

10.1016/j.fuel.2021.122263 ◽

2022 ◽

Vol 310 ◽

pp. 122263

Author(s):

Konstantina Tsigkou ◽

Michael Kornaros

Keyword(s):

Packed Bed ◽

Olive Mill Wastewater ◽

Packed Bed Reactor ◽

High Rate ◽

Three Phase ◽

Olive Mill

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Bacterial Diversity of Breast Milk in Healthy Spanish Women: Evolution from Birth to Five Years Postpartum

Nutrients ◽

10.3390/nu13072414 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2414

Author(s):

Laura Sanjulián ◽

Alexandre Lamas ◽

Rocío Barreiro ◽

Alberto Cepeda ◽

Cristina A. Fente ◽

...

Keyword(s):

Breast Milk ◽

16S Rrna ◽

Human Milk ◽

Alpha Diversity ◽

Amplicon Sequencing ◽

Maternal Body Mass Index ◽

16S Rrna Amplicon Sequencing ◽

Spanish Women ◽

Calcium Magnesium ◽

Abundant Genus

The objective of this work was to characterize the microbiota of breast milk in healthy Spanish mothers and to investigate the effects of lactation time on its diversity. A total of ninety-nine human milk samples were collected from healthy Spanish women and were assessed by means of next-generation sequencing of 16S rRNA amplicons and by qPCR. Firmicutes was the most abundant phylum, followed by Bacteroidetes, Actinobacteria, and Proteobacteria. Accordingly, Streptococcus was the most abundant genus. Lactation time showed a strong influence in milk microbiota, positively correlating with Actinobacteria and Bacteroidetes, while Firmicutes was relatively constant over lactation. 16S rRNA amplicon sequencing showed that the highest alpha-diversity was found in samples of prolonged lactation, along with wider differences between individuals. As for milk nutrients, calcium, magnesium, and selenium levels were potentially associated with Streptococcus and Staphylococcus abundance. Additionally, Proteobacteria was positively correlated with docosahexaenoic acid (DHA) levels in breast milk, and Staphylococcus with conjugated linoleic acid. Conversely, Streptococcus and trans-palmitoleic acid showed a negative association. Other factors such as maternal body mass index or diet also showed an influence on the structure of these microbial communities. Overall, human milk in Spanish mothers appeared to be a complex niche shaped by host factors and by its own nutrients, increasing in diversity over time.

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Connecting structure to function with the recovery of over 1000 high-quality metagenome-assembled genomes from activated sludge using long-read sequencing

Nature Communications ◽

10.1038/s41467-021-22203-2 ◽

2021 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Caitlin M. Singleton ◽

Francesca Petriglieri ◽

Jannie M. Kristensen ◽

Rasmus H. Kirkegaard ◽

Thomas Y. Michaelsen ◽

...

Keyword(s):

16S Rrna ◽

Wastewater Treatment Plants ◽

In Situ Hybridisation ◽

Amplicon Sequencing ◽

Rrna Genes ◽

Fluorescence In Situ Hybridisation ◽

Sequencing Data ◽

High Quality ◽

16S Rrna Amplicon Sequencing ◽

Long Read

AbstractMicroorganisms play crucial roles in water recycling, pollution removal and resource recovery in the wastewater industry. The structure of these microbial communities is increasingly understood based on 16S rRNA amplicon sequencing data. However, such data cannot be linked to functional potential in the absence of high-quality metagenome-assembled genomes (MAGs) for nearly all species. Here, we use long-read and short-read sequencing to recover 1083 high-quality MAGs, including 57 closed circular genomes, from 23 Danish full-scale wastewater treatment plants. The MAGs account for ~30% of the community based on relative abundance, and meet the stringent MIMAG high-quality draft requirements including full-length rRNA genes. We use the information provided by these MAGs in combination with >13 years of 16S rRNA amplicon sequencing data, as well as Raman microspectroscopy and fluorescence in situ hybridisation, to uncover abundant undescribed lineages belonging to important functional groups.

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Enhancement of the anaerobic digestion of olive mill wastewater by the removal of phenolic inhibitors

Process Biochemistry ◽

10.1016/0032-9592(92)80023-v ◽

1992 ◽

Vol 27 (4) ◽

pp. 231-237 ◽

Cited By ~ 86

Author(s):

R. Borja ◽

A. Martin ◽

R. Maestro ◽

J. Alba ◽

J.A. Fiestas

Keyword(s):

Anaerobic Digestion ◽

Olive Mill Wastewater ◽

Olive Mill

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Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

Applied Sciences ◽

10.3390/app11031293 ◽

2021 ◽

Vol 11 (3) ◽

pp. 1293

Author(s):

Ana Eusébio ◽

André Neves ◽

Isabel Paula Marques

Keyword(s):

Anaerobic Digestion ◽

Microbial Communities ◽

Volatile Fatty Acids ◽

Olive Mill Wastewater ◽

Organic Load ◽

Rrna Gene ◽

Bacterial Populations ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing ◽

Olive Mill

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.

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