Emergence of different Acinetobacter baumannii clones in a Croatian hospital and correlation with antibiotic susceptibility

2017 ◽  
Vol 10 ◽  
pp. 213-218 ◽  
Author(s):  
Ranko Ladavac ◽  
Branka Bedenić ◽  
Mirna Vranić-Ladavac ◽  
Nada Barišić ◽  
Natalie Karčić ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antibiotic Susceptibility

The Changing Patterns of Antibiotic Susceptibility for Acinetobacter baumannii in Pediatric Burn Patients

2008 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
Sang-Min Lee ◽  
So-Yeon Lee ◽  
Young-Ho Kim ◽  
Kyu-Man Lee ◽  
Young-Chul Jang ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antibiotic Susceptibility ◽  
Burn Patients ◽  
Changing Patterns

scholarly journals Antibiotic Susceptibility, Clonality, and Molecular Characterization of Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii from Washington DC

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Garima Bansal ◽  
Rachelle Allen-McFarlane ◽  
Broderick Eribo
Keyword(s):  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Antibiotic Susceptibility ◽  
Carbapenem Resistance ◽  
The United States ◽  
Pcr Analysis ◽  
Washington Dc ◽  
Antibiotic Resistant ◽  
Group 4 ◽  
Carbapenem Resistant

The occurrence of carbapenem-resistant (CR) strains of Acinetobacter baumannii is reported to contribute to the severity of several nosocomial infections, especially in critically ill patients in intensive care units. The present study aims to determine the antibiotic susceptibility, clonality, and genetic mechanism of carbapenem resistance in twenty-eight Acinetobacter baumannii isolates from four hospitals in Washington DC. The antibiotic susceptibility of the isolates was determined by VITEK 2 analyses, while PCR was used to examine the presence of antibiotic-resistant genes and mobile genetic elements. Trilocus multiplex-PCR was used along with pulsed-field gel electrophoresis (PFGE) for strain typing and for accessing clonal relationships among the isolates. Antimicrobial susceptibility testing indicated that 46% of the isolates were carbapenem-resistant and possessed MDR and XDR phenotypes. PFGE clustered the 28 isolates into seven clonal (C1–C7) complexes based on >75% similarity cut-off. Thirty-six percent of the isolates belonged to international clone II, while 29% were assigned to Group 4 by trilocus multiplex-PCR. Although the blaOXA-51-like gene was found in all the isolates, only 36% were positive for the blaOXA-23-like gene. PCR analysis also found a metallo-β-lactamase (MBL) gene (blaVIM) in 71% of the isolates. Of the 13 CR isolates, 8 were PCR positive for both blaVIM and blaOXA-23-like genes, while 5 harbored only blaVIM gene. This study revealed the emergence of VIM carbapenemase-producing A. baumannii isolates, which has not been previously reported in the United States.

scholarly journals Aerosolized Hypertonic Saline Hinders Biofilm Formation to Enhance Antibiotic Susceptibility of Multidrug-Resistant Acinetobacter baumannii

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1115
Author(s):  
Hui-Ling Lin ◽  
Chen-En Chiang ◽  
Mei-Chun Lin ◽  
Mei-Lan Kau ◽  
Yun-Tzu Lin ◽  
...  
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Acinetobacter Baumannii ◽  
Hypertonic Saline ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Therapeutic Strategy ◽  
Extracellular Polymeric Substances ◽  
Multidrug Resistant ◽  
Biofilm Disruption

Limited therapeutic options are available for multidrug-resistant Acinetobacter baumannii (MDR-AB), and the development of effective treatments is urgently needed. The efficacy of four aerosolized antibiotics (gentamicin, amikacin, imipenem, and meropenem) on three different MDR-AB strains was evaluated using hypertonic saline (HS, 7 g/100 mL) as the aerosol carrier. HS aerosol effectively hindered biofilm formation by specific MDR-AB strains. It could also interrupt the swarming dynamics of MDR-AB and the production of extracellular polymeric substances, which are essential for biofilm progression. Biofilms protect the microorganisms from antibiotics. The use of HS aerosol as a carrier resulted in a decreased tolerance to gentamicin and amikacin in the biofilm-rich MDR-AB. Moreover, we tested the aerosol characteristics of antibiotics mixed with HS and saline, and results showed that HS enhanced the inhaled delivery dose with a smaller particle size distribution of the four antibiotics. Our findings demonstrate the potential of using “old” antibiotics with our “new” aerosol carrier, and potentiate an alternative therapeutic strategy to eliminate MDR-AB infections from a biofilm-disruption perspective.

scholarly journals Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification in Acinetobacter baumannii

2020 ◽  
Vol 8 ◽  
Author(s):  
Shuang Liu ◽  
Guangtao Huang ◽  
Yali Gong ◽  
Xiaojun Jin ◽  
Yudan Meng ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Antibiotic Susceptibility ◽  
Carbapenem Resistance ◽  
Molecular Diagnostic ◽  
Promising Alternative ◽  
Accurate Detection ◽  
Antibiotic Susceptibility Test ◽  
Medical University

Abstract Background Acinetobacter baumannii (A. baumannii) is one of the pivotal pathogens responsible for nosocomial infections, especially in patients with low immune response, and infection with carbapenem-resistant A. baumannii has been increasing in recent years. Rapid and accurate detection of carbapenem-resistance genes in A. baumannii could be of immense help to clinical staff. Methods In this study, a 15-μL reaction system for recombinase polymerase amplification (RPA) was developed and tested. We collected 30 clinical isolates of A. baumannii from the Burn Institute of Southwest Hospital of Third Military Medical University (Army Medical University) for 6 months and tested antibiotic susceptibility using the VITEK 2 system. A. baumannii was detected based on the blaOXA-51 gene by PCR, qPCR and 15 μL-RPA, respectively. Sensitivity and specificity were evaluated. In addition, PCR and 15 μL-RPA data for detecting the carbapenem-resistance gene blaOXA-23 were comparatively assessed. Results The detection limit of the blaOXA-51 gene by 15 μL RPA was 2.86 CFU/ml, with sensitivity comparable to PCR and qPCR. No positive amplification signals were detected in non-Acinetobacter isolates, indicating high specificity. However, only 18 minutes were needed for the 15 μL RPA assay. Furthermore, an antibiotic susceptibility test showed that up to 90% of A. baumannii strains were resistant to meropenem and imipenem; 15 μL RPA data for detecting blaOXA-23 showed that only 10% (n = 3) of A. baumannii isolates did not show positive amplification signals, and the other 90% of (n = 27) isolates were positive, corroborating PCR results. Conclusion We demonstrated that the new 15 μL RPA assay for detecting blaOXA-23 in A. baumannii is faster and simpler than qPCR and PCR. It is a promising alternative molecular diagnostic tool for rapid and effective detection of A. baumannii and drug-resistance genes in the field and point-of-care testing.

Application of a Bioinformatics Method on Detecting of Acinetobacter baumannii Carrying Metallo-β-Lactamases Isolated from Inpatients

2014 ◽  
Vol 881-883 ◽  
pp. 335-338
Author(s):  
Lin Lin Ma ◽  
Ali Huang ◽  
Wei Chu ◽  
Xiao Han Li ◽  
Ming Cheng Li
Keyword(s):  
Acinetobacter Baumannii ◽  
Antimicrobial Susceptibility ◽  
Potential Risk ◽  
Gel Electrophoresis ◽  
Antibiotic Susceptibility ◽  
Screening Method ◽  
Pulsed Field Gel Electrophoresis ◽  
Plasmid Analysis ◽  
Antibiotic Susceptibility Test ◽  
Susceptibility Patterns

The aim of the study was to investigate the prevalence and charateristics of Acinetobacter baumannii producing MBLs isolated from the several wards of the affiliated hospital in Jilin regions. The bioinformatics technology was used to design primers, sequence and blast, in conjunction with antibiotic susceptibility test and MBL screening method. The total of 119 were isolated from three wards and 88 strains (73.9 %) produced MBLs, the number of 31 strains (26.1%) didnt produce MBLs. All MBLs isolates showed same antimicrobial susceptibility patterns. Among Acinetobacter baumannii producing MBLs, 78 strains (88.9%) were positive for blaOXA-23 gene, 10 strains (11.1%) contained blaVIM-2 gene. Plasmid profiles and pulsed-field gel electrophoresis were used to study the relatedness of isolates. All MBLs isolates were closely related by PFGE and plasmid analysis. This study confirmed the potential risk of spreading of Acinetobacter baumannii producing MBLs with inter-and intrahospital clonal transfer of patients.

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