scholarly journals CCL2-Mediated Reversal of Impaired Skin Wound Healing in Diabetic Mice by Normalization of Neovascularization and Collagen Accumulation

2019 ◽  
Vol 139 (12) ◽  
pp. 2517-2527.e5 ◽  
Author(s):  
Yuko Ishida ◽  
Yumi Kuninaka ◽  
Mizuho Nosaka ◽  
Machi Furuta ◽  
Akihiko Kimura ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Diabetic Mice ◽  
Skin Wound Healing ◽  
Collagen Accumulation

scholarly journals Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2554
Author(s):  
Marek Konop ◽  
Anna K. Laskowska ◽  
Mateusz Rybka ◽  
Ewa Kłodzińska ◽  
Dorota Sulejczak ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

scholarly journals Improvement of skin wound healing in diabetic mice by kinin B2 receptor blockade

2015 ◽  
Vol 130 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Dorinne Desposito ◽  
Catherine Chollet ◽  
Christopher Taveau ◽  
Vincent Descamps ◽  
François Alhenc-Gelas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Models ◽  
Receptor Activation ◽  
Skin Wound ◽  
Receptor Blockade ◽  
Fibroblast Proliferation ◽  
Diabetic Mice ◽  
Skin Wound Healing ◽  
Normal Wound Healing

Kinin B2 receptor activation impairs skin wound healing in mice, in part through imbalance of keratinocyte/fibroblast proliferation. Such activation occurs in diabetes and contributes to delaying wound healing. B2 receptor blockade restores the normal wound healing pattern in mouse models of diabetes.

Oral administration of antioxidants improves skin wound healing in diabetic mice

2016 ◽  
Vol 24 (6) ◽  
pp. 981-993 ◽  
Author(s):  
Ana Flávia Marçal Pessoa ◽  
Juliana Costa Florim ◽  
Hosana Gomes Rodrigues ◽  
Vinicius Andrade-Oliveira ◽  
Simone A. Teixeira ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Administration ◽  
Skin Wound ◽  
Diabetic Mice ◽  
Skin Wound Healing

Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice

2017 ◽  
Vol 79 (3) ◽  
pp. e15-e19 ◽  
Author(s):  
Yinjia Ding ◽  
Lei Cui ◽  
Qiming Zhao ◽  
Weiqiang Zhang ◽  
Huafeng Sun ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Diabetic Mice ◽  
Skin Wound Healing ◽  
Platelet Rich Fibrin

scholarly journals A low dose of simvastatin enhanced the therapeutic efficacy of mesenchymal stem cell (MSC) transplantation in skin wound healing in diabetic mice associated with increases in pAkt, SDF-1, and angiogenesis

2019 ◽  
Author(s):  
Supakanda Sukpat ◽  
Nipan Israsena ◽  
Jutamas Wongphoom ◽  
Praewphan Ingrungruanglert ◽  
Tao Ming Sim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Efficacy ◽  
Wound Closure ◽  
Low Dose ◽  
Skin Wound ◽  
Diabetic Wound ◽  
Diabetic Mice ◽  
Skin Wound Healing ◽  
Wound Induction ◽  
Diabetic Wound Healing

AbstractPurposeWe aimed to determine the possible mechanisms of underlying the effects of low dose simvastatin on enhancing the therapeutic efficacy of MSC transplantation in diabetic wound healing.MethodsBalb/c nude mice were divided into five groups:- control mice (CON), diabetic mice (DM), diabetic mice pretreated with low-dose simvastatin (DM+SIM), diabetic mice implanted with MSCs (DM+MSCs) and diabetic mice pretreated with low-dose simvastatin and implanted with MSCs (DM+MSCs+SIM). Seven days before wound induction, low dose simvastatin was orally administered to the DM+SIM and DM+MSCs+SIM groups. Eleven weeks after the induction of diabetes, all mice were given bilateral full-thickness excisional back skin wounds.ResultsBy comparing the DM+MSCs+SIM and DM+MSCs groups, the results showed that on day 14; the wound closure (%WC) and capillary vascularity (%CV) in the DM+MSCs+SIM group were significantly increased compared to those in the DM+MSCs group. In addition, by using immunohistochemical techniques, it was also shown that the expression of SDF-1, a chemotactic factor regulating the migration of stem cells, in the DM+SIM+MSCs group was increased compared with that in the DM+MSCs group. Furthermore, using phospho-Akt (S473) Pan Specific DuoSet IC ELISA (R&D Systems, USA) kits, the increased tissue Akt levels were found in the DM+SIM+MSCs group but not in the other groups.ConclusionsOur study suggests that a low dose of simvastatin enhanced the therapeutic efficacy of MSCs in diabetic wound healing, and this effect was associated with increases in pAkt levels, SDF-1 levels, and angiogenesis, and improved wound closure.

scholarly journals The role of bone marrow mesenchymal stromal cell derivatives in skin wound healing in diabetic mice

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0177533 ◽  
Author(s):  
Tomas de Mayo ◽  
Paulette Conget ◽  
Silvia Becerra-Bayona ◽  
Claudia L. Sossa ◽  
Virgilio Galvis ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Skin Wound ◽  
Diabetic Mice ◽  
Skin Wound Healing
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