Correlation of increased antioxidation with the phenolic compound and amino acids contents of Camellia sinensis leaf extracts following ultra high pressure extraction

AbstractAmino acids (AAs) and one-carbon (1-C) compounds are involved in a range of key metabolic pathways, and mediate numerous health and disease processes in the human body. Previous assays have quantified a limited selection of these compounds. Here, we describe an analytical method for the simultaneous quantification of 37 1-C metabolites, amino acids, precursors, and plasmalogens using reversed-phase ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Compound extraction from human plasma was tested manually before being robotically automated. The final analytical panel was validated on human plasma samples. Our automated and multiplexed method holds promise for application to large cohort studies.

Download Full-text

Identification and Antioxidant Activity of Flavonoids Extracted from Xinjiang Jujube (Ziziphus jujube Mill.) Leaves with Ultra-High Pressure Extraction Technology

Molecules ◽

10.3390/molecules24010122 ◽

2018 ◽

Vol 24 (1) ◽

pp. 122 ◽

Cited By ~ 13

Author(s):

Li Zhang ◽

Pengzhan Liu ◽

Linlin Li ◽

Ying Huang ◽

Yunfeng Pu ◽

...

Keyword(s):

High Pressure ◽

Large Scale ◽

Radical Scavenging ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Solid Ratio ◽

Ultra High Pressure ◽

Assisted Extraction ◽

Pressure Extraction

In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining the maximum flavonoid yield from Xinjiang jujube (Ziziphus jujuba Mill.) leaves (XJL) were optimized by response surface methodology (RSM). Box–Behnken design (BBD) was applied to evaluate the effects of four variables (extraction temperature, pressure, time and liquid-to-solid ratio) on flavonoid yield. The results showed that the optimal flavonoid yield (25.45 ± 0.21 mg/g) was derived at 50.0 °C, 342.39 MPa, 11.56 min, and a liquid-to-solid ratio of 43.95 mL/g. Eight compounds were tentatively identified and quantified as kaempferol and quercetin glycosides with UPLC-ESI-MS. Compared to ultrasound-assisted extraction (UAE), UHPE can obtain higher concentrations of total flavonoids and stronger DPPH and ABTS radical-scavenging activities in a much shorter time. Therefore, UHPE is an alternative to UAE for obtaining flavonoids from XJL, which may be an optional method for large-scale industrial flavonoid extraction from XJL.

Download Full-text

Optimization of Ultra-High Pressure Extraction Process of Polysaccharides from Dendrobium Candidum by Response Surface Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1796 ◽

2012 ◽

Vol 550-553 ◽

pp. 1796-1800 ◽

Cited By ~ 1

Author(s):

Xue Tao ◽

Yao Zhan ◽

Qiu Yan Zhou ◽

Feng Qin Feng ◽

Yong Yu

Keyword(s):

High Pressure ◽

Response Surface ◽

Response Surface Method ◽

Extraction Process ◽

Holding Time ◽

Solid Ratio ◽

Ultra High Pressure ◽

Surface Method ◽

Pressure Extraction ◽

Dendrobium Candidum

Ultra-high pressure extraction technique was used to extract polysaccharides from Dendrobium candidum. Extraction pressure, pressure holding time and liquid-solid ratio were found to have a significant influence on the polysaccharides extraction and were optimized by the response surface method (RSM). The obtained optimum conditions were: extraction pressure 445.28MPa, pressure holding time 6.7 minutes and liquid-solid ratio 237.91ml/g. The maximum polysaccharide yield could reach 596.75mg/g under these conditions. The ultra-high pressure extraction process was a suitable method for its higher yield and shorter extraction time compared to the water-heating extraction.

Download Full-text