Identification and Antioxidant Activity of Flavonoids Extracted from Xinjiang Jujube (Ziziphus jujube Mill.) Leaves with Ultra-High Pressure Extraction Technology

In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining the maximum flavonoid yield from Xinjiang jujube (Ziziphus jujuba Mill.) leaves (XJL) were optimized by response surface methodology (RSM). Box–Behnken design (BBD) was applied to evaluate the effects of four variables (extraction temperature, pressure, time and liquid-to-solid ratio) on flavonoid yield. The results showed that the optimal flavonoid yield (25.45 ± 0.21 mg/g) was derived at 50.0 °C, 342.39 MPa, 11.56 min, and a liquid-to-solid ratio of 43.95 mL/g. Eight compounds were tentatively identified and quantified as kaempferol and quercetin glycosides with UPLC-ESI-MS. Compared to ultrasound-assisted extraction (UAE), UHPE can obtain higher concentrations of total flavonoids and stronger DPPH and ABTS radical-scavenging activities in a much shorter time. Therefore, UHPE is an alternative to UAE for obtaining flavonoids from XJL, which may be an optional method for large-scale industrial flavonoid extraction from XJL.

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Optimization of ultrasound-assisted extraction of bioactive compounds from Gomphrena celosioides Mart. using response surface methodology

Bulletin of the Chemical Society of Ethiopia ◽

10.4314/bcse.v34i2.3 ◽

2020 ◽

Vol 34 (2) ◽

pp. 237-248

Author(s):

N. T. H. Yen ◽

L. P. T. Quoc

Keyword(s):

Bioactive Compounds ◽

Radical Scavenging ◽

Extraction Time ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Gomphrena Celosioides ◽

Experimental Values

In this study, ultrasound-assisted extraction (UAE) was used to extract bioactive compounds from Gomphrena celosioides Mart. Central composite face design (CCF) was used to optimize the influences of extraction factors on total phenolics content (TPC), total flavonoids content (TFC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging capacity (DPPH-RSC). The results obtained showed that all factors (solvent to solid ratio, extraction time and extraction temperature) strongly affected TPC, TFC, and DPPH-RSC. The optimal extraction conditions for TPC (3.123 mg GAE/g DW), TFC (1.736 mg QE/g DW), and DPPH-RSC (64.118%) were solvent to solid ratio of 27.3/1 (mL/g), extraction time of 40.2 min, and extraction temperature of 69 oC. In addition, under the optimal conditions, the results pointed out that the experimental values agreed with those predicted. Hence, this model has successfully optimized the extraction conditions for TPC, TFC and DPPH-RSC of Gomphrena celosioides extract. KEY WORDS: Antioxidant activity, CCF, Flavonoids, Model, Phenolic compounds, Regression equation Bull. Chem. Soc. Ethiop. 2020, 34(2), 237-248 DOI: https://dx.doi.org/10.4314/bcse.v34i2.3

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Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity

Antioxidants ◽

10.3390/antiox8100425 ◽

2019 ◽

Vol 8 (10) ◽

pp. 425 ◽

Cited By ~ 4

Author(s):

Mengyang Hou ◽

Wenzhong Hu ◽

Aosheng Wang ◽

Zhilong Xiu ◽

Yusheng Shi ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Pteris Cretica ◽

Nitric Oxide Radical ◽

Ultrasonic Assisted ◽

Ic50 Values

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from Pteris cretica L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50 values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from P. cretica.

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Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽

10.3390/antiox8070200 ◽

2019 ◽

Vol 8 (7) ◽

pp. 200 ◽

Cited By ~ 5

Author(s):

Yue Zhou ◽

Xiao-Yu Xu ◽

Ren-You Gan ◽

Jie Zheng ◽

Ya Li ◽

...

Keyword(s):

Extraction Method ◽

Natural Antioxidants ◽

Extraction Time ◽

Antioxidant Compounds ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

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Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of Malus toringoides Using Response Surface Methodology

Processes ◽

10.3390/pr7050270 ◽

2019 ◽

Vol 7 (5) ◽

pp. 270 ◽

Cited By ~ 1

Author(s):

Qiang Ye ◽

Li Guo ◽

Hongmei Liu ◽

Yushi Liu ◽

Cunyan Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Response Surface Methodology ◽

Response Surface ◽

Umbilical Vein ◽

Extraction Temperature ◽

Least Squares Regression ◽

Protective Activity ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction

Ultrasonic-assisted extraction (UAE) was optimized using response surface methodology (RSM) to maintain the cyto-protective activity of M.toringoides against oxidative stress. The optimal conditions for UAE were a 58 mL/g liquid-solid ratio, a 38 °C extraction temperature, an 85% solvent concentration, and a 19-min extraction time, which resulted in a protection rate of 54.57% against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). These results were comparable to the predicted value of 53.75%. The extracts showed excellent antioxidant activity, and phlorizin was detected in the dried leaves of Malus.toringoides. The highest yield of phlorizin (101.239 mg/g) was also obtained using these conditions. Taken together, these results showed that the method successfully integrated RSM and partial least squares regression methods to optimize M.toringoides extraction to yield the highest cyto-protective activity and effectively increase the yield of phlorizin from M.toringoides.

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Optimization of Ultra-High Pressure Extraction Process of Polysaccharides from Dendrobium Candidum by Response Surface Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1796 ◽

2012 ◽

Vol 550-553 ◽

pp. 1796-1800 ◽

Cited By ~ 1

Author(s):

Xue Tao ◽

Yao Zhan ◽

Qiu Yan Zhou ◽

Feng Qin Feng ◽

Yong Yu

Keyword(s):

High Pressure ◽

Response Surface ◽

Response Surface Method ◽

Extraction Process ◽

Holding Time ◽

Solid Ratio ◽

Ultra High Pressure ◽

Surface Method ◽

Pressure Extraction ◽

Dendrobium Candidum

Ultra-high pressure extraction technique was used to extract polysaccharides from Dendrobium candidum. Extraction pressure, pressure holding time and liquid-solid ratio were found to have a significant influence on the polysaccharides extraction and were optimized by the response surface method (RSM). The obtained optimum conditions were: extraction pressure 445.28MPa, pressure holding time 6.7 minutes and liquid-solid ratio 237.91ml/g. The maximum polysaccharide yield could reach 596.75mg/g under these conditions. The ultra-high pressure extraction process was a suitable method for its higher yield and shorter extraction time compared to the water-heating extraction.

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Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS

Molecules ◽

10.3390/molecules25194507 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4507

Author(s):

Zhen Li ◽

Zongcai Tu ◽

Hui Wang ◽

Lu Zhang

Keyword(s):

Chinese Herb ◽

Ceratophyllum Demersum ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Glucosidase Inhibitors ◽

Assisted Extraction ◽

Ic50 Value ◽

Ultrasound Assisted

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

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Optimization of Ultrasound-Assisted Extraction of Polysaccharides from the Chinese Medicinal Plant Rhodiola Sachalinensis A. BOR

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.448-453.1085 ◽

2013 ◽

Vol 448-453 ◽

pp. 1085-1088

Author(s):

Chuan Jun Zhang ◽

Feng Lin Li

Keyword(s):

Medicinal Plant ◽

Extraction Time ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Rhodiola Sachalinensis ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Chinese Medicinal Plant

Ultrasound-assisted extraction (UAE) of polysaccharides from the Chinese medicinal plantRhodiola sachalinensisA. BOR (PRs) was studied. The effects of four factors (extraction time, ultrasonic power, liquid-solid ratio and extraction temperature) on the extraction yield of PRs were investigated by the single-factor test, and the optimum UAE conditions were investigated by the orthogonal test. The results demonstrated that the optimum UAE conditions for PRs were as followings: extraction time of 30 min, ultrasonic power of 85 W, liquid-solid ratio of 45 ml/g, and extraction temperature of 45°C. The present data might provide a theoretic base for full utilization ofRhodiola sachalinensisA. BOR.

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Response Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from QingLi Cao and Antioxidant Activities

The Open Biotechnology Journal ◽

10.2174/1874070701509010134 ◽

2015 ◽

Vol 9 (1) ◽

pp. 134-142 ◽

Cited By ~ 2

Author(s):

Xudong Jiang ◽

YaoLing Liao ◽

GuiXi Lu ◽

Zhike Xiao

Keyword(s):

Response Surface ◽

Antioxidant Activities ◽

Reducing Power ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

An ultrasound-assisted extraction technique was used to extract the total flavonoids from QingLi Cao. The optimal conditions were ethanol concentration 59.20%, liquid-to-solid ratio 31.15 mL/g, extraction time 57.42 min and extraction temperature 58.57°C, which were determined using response surface methodology. The antioxidant activities including reducing power, ABTS+, DPPH, superoxide anion and hydroxyl radical were evaluated, which suggested significant antioxidant activities.

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Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

BioMed Research International ◽

10.1155/2020/3497107 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Dinh-Chuong Pham ◽

Hoang-Chinh Nguyen ◽

Thanh-Hang Le Nguyen ◽

Hoang-Linh Ho ◽

Thien-Kim Trinh ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Biological Activities ◽

Radical Scavenging ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Anticancer Activities ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.

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ULTRASOUND-ASSISTED EXTRACTION OF TOTAL FLAVONOIDS FROM LEAVES OF SYRINGA OBLATA LINDL.

Latin American Applied Research - An international journal ◽

10.52292/j.laar.2014.430 ◽

2014 ◽

Vol 44 (2) ◽

pp. 131-135

Author(s):

Z. SHENG ◽

Y. WANG ◽

P. WAN ◽

Y. LI

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Plant Sample ◽

Solvent Ratio ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Assisted Extraction ◽

Ultrasound Assisted

Leaves of Syringa Oblata Lindl. possess some important biological activities, such as antibacterial, antiviral, anti-inflammatory and antioxidant activities. These biological properties are mainly attributed to total flavonoids content. In this paper, ultrasound-assisted extraction of total flavonoids from leaves of Syringa Oblata Lindl. was studied. Effects of several experimental parameters, such as concentration of extracting solvent, ratio of liquid to material, extraction temperature, and time of sonication on extraction efficiencies of total flavonoids were evaluated. The best extraction conditions were: 1 g plant sample with 20 mL of 50% ethanol, at 60 °C for 50 min, obtaining a yield of total flavonoids of 92.00 ± 0.87 mg/g of plant. The results indicated that high amounts of total flavonoids can be extracted from leaves of Syringa Oblata Lindl. by ultrasound-assisted extraction technology.

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