Toxicity of phenolic compounds to entomopathogenic nematodes: A case study with Heterorhabditis bacteriophora exposed to lentisk (Pistacia lentiscus) extracts and their chemical components

2019 ◽  
Vol 160 ◽  
pp. 43-53 ◽  
Author(s):  
Velayudhan Satheeja Santhi ◽  
Liora Salame ◽  
Hussein Muklada ◽  
Hassan Azaizeh ◽  
Manal Haj-Zaroubi ◽  
...  
Author(s):  
N.L. Bell ◽  
L.T. Aalders ◽  
T.C. Rohan

Entomopathogenic nematodes (EPNs) have been used experimentally to control insects in pastures and in this study we investigated the use of EPNs against clover root weevil. We tested the ability of two EPNs (Steinernema carpocapsae and Heterorhabditis zealandica) to control soil-dwelling stages of clover root weevil in a Waikato pasture Keywords: Sitona lepidus, Heterorhabditis bacteriophora, larvae, pupae, Galleria, wax moth


2016 ◽  
Vol 3 (6) ◽  
pp. 355-365
Author(s):  
Funmilayo Adelowo ◽  
Oluwole Oladeji

This present work is aimed at determining the chemical components in Senna alata (L.) Roxb. (Fabales: Fabaceae), that contains a large array of phenolic compounds which make it useful as antifungal and antibacterial. Despite a notable success in the recent researches, yet, the quantification of the chemical components has not been actually specified. There is a claim by local people that S. alata can cure fungi and bacteria related diseases. Therefore, there is a need to ascertain this claim by examining the bioactive components present in the plant. The Gas Chromatography (GC) analysis was performed using a Clarus 500 Perkin Elmer gas chromatography equipped with an Elite-5 capillary column with the addition of an absolute alcohol. The antimicrobial analysis was carried out using Agar Disc Diffusion Method. In the GC chromatogram, four prominent peaks were observed in leaf chromatogram, three peaks in flower chromatogram and five peaks in fruit chromatogram, respectively. The flower contained the highest amount of cinnamic acid (52.91%) and the fruit contained the least (17.03%). The presence of some bioactive metabolites could be responsible for the observed antifungal and antibacterial activities on the susceptible organisms studied. It could be concluded that this plant is natural sources of antimicrobial substances of high importance and are useful in chemotherapy.


2012 ◽  
Vol 11 (3) ◽  
pp. 131-136 ◽  
Author(s):  
Asma Cherbal ◽  
Mohamed Kebieche ◽  
Khodir Madani ◽  
Hala El-Adawi

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 33
Author(s):  
Carmen X. Luzuriaga-Quichimbo ◽  
José Blanco-Salas ◽  
Carlos E. Cerón-Martínez ◽  
Juan Carlos Alías-Gallego ◽  
Trinidad Ruiz-Téllez

Traditional medicine is especially important in the treatment of neglected tropical diseases because it is the way the majority of populations of affected countries manage primary healthcare. We present a case study that can serve as an example that can be replicated by others in the same situation. It is about the validation of a local remedy for myasis in Amazonian Ecuador, which is contrasted by bibliographic chemical reviews and in silico activity tests. We look for scientific arguments to demonstrate the reason for using extracts of Lonchocarpus utilis against south American myasis (tupe). We provide a summary of the isoflavonoids, prenylated flavonoids, chalcones, and stilbenes that justify the action. We make modeling predictions on the affinity of eight chemical components and enzyme targets using Swiss Target Prediction software. We conclude that the effects of this extract can be reasonably attributed to an effect of the parasite that causes the disease, similar to the one produced by synthetic drugs used by conventional medicine (e.g., Ivermectine).


2020 ◽  
Vol 6 (4) ◽  
pp. 359
Author(s):  
Jiří Nermuť ◽  
Jana Konopická ◽  
Rostislav Zemek ◽  
Michal Kopačka ◽  
Andrea Bohatá ◽  
...  

Entomopathogenic nematodes and fungi are globally distributed soil organisms that are frequently used as bioagents in biological control and integrated pest management. Many studies have demonstrated that the combination of biocontrol agents can increase their efficacy against target hosts. In our study, we focused on another potential benefit of the synergy of two species of nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, and the fungus Isaria fumosorosea. According to our hypothesis, these nematodes may be able to disseminate this fungus into the environment. To test this hypothesis, we studied fungal dispersal by the nematodes in different arenas, including potato dextrose agar (PDA) plates, sand heaps, sand barriers, and glass tubes filled with soil. The results of our study showed, for the first time, that the spreading of both conidia and blastospores of I. fumosorosea is significantly enhanced by the presence of entomopathogenic nematodes, but the efficacy of dissemination is negatively influenced by the heterogeneity of the testing arena. We also found that H. bacteriophora spread fungi more effectively than S. feltiae. This phenomenon could be explained by the differences in the presence and persistence of second-stage cuticles or by different foraging behavior. Finally, we observed that blastospores are disseminated more effectively than conidia, which might be due to the different adherence of these spores (conidia are hydrophobic, while blastospores are hydrophilic). The obtained results showed that entomopathogenic nematodes (EPNs) can enhance the efficiency of fungal dispersal.


2020 ◽  
Vol 21 (14) ◽  
pp. 4986 ◽  
Author(s):  
Axelle Septembre-Malaterre ◽  
Mahary Lalarizo Rakoto ◽  
Claude Marodon ◽  
Yosra Bedoui ◽  
Jessica Nakab ◽  
...  

Traditional remedies have been used for thousand years for the prevention and treatment of infectious diseases, particularly in developing countries. Of growing interest, the plant Artemisia annua, known for its malarial properties, has been studied for its numerous biological activities including metabolic, anti-tumor, anti-microbial and immunomodulatory properties. Artemisia annua is very rich in secondary metabolites such as monoterpenes, sesquiterpenes and phenolic compounds, of which the biological properties have been extensively studied. The purpose of this review is to gather and describe the data concerning the main chemical components produced by Artemisia annua and to describe the state of the art about the biological activities reported for this plant and its compounds beyond malaria.


Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Anwar Bilgrami ◽  
Randy Gaugler

AbstractCentrifugation, desiccation, agitation, and handling of entomopathogenic nematodes in the laboratory during isolation, culture, storage, formulation and experimentation, influences nematode ability to tolerate adverse environmental conditions. Stress imposed by centrifugation (5-60 min), desiccation (3-9 days), agitation (3-24 h), and handling (2-10 times) reduced stress and heat tolerance in Heterorhabditis bacteriophora and Steinernema carpocapsae. Short durations of stresses (e.g., 5 min of centrifugation, 3-5 days of desiccation, 3 h of agitation and 2-4 times of handling) did not affect nematodes, whereas prolonged durations (e. g., 10-60 min of centrifugation, 7-9 days of desiccation, 6-24 h of agitation and 6-10 times of handling) significantly decreased heat tolerance. Steinernema carpocapsae tolerated stress comparatively better than H. bacteriophora by showing a significantly greater degree of heat tolerance. This study provides a basis to investigate further the effects of physical and chemical stresses in order to minimise handling of laboratory nematodes and reduce disruptions of their normal function and behaviour.


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