Intra-osseous application of plasma rich in growth factors to treat acute cartilage defects in rabbits: evaluation of blood biomarkers (hyaluronic acid and collagen type II cleavage epitope)

2021 ◽  
Vol 29 ◽  
pp. S409-S410
Author(s):  
M. Torres Torrillas ◽  
E. Damiá ◽  
B. Cuervo ◽  
D. Chicharro ◽  
A. Del Romero ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factors ◽  
Collagen Type ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Blood Biomarkers

scholarly journals Comparative Analysis of Adipose-Derived Stromal Cells and Their Secretome for Auricular Cartilage Regeneration

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
Soo-Keun Kong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Stromal Cells ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage ◽  
Adipose Derived Stromal Cells

Adipose-derived stromal cells (ADSCs) can repair auricular cartilage defects. Furthermore, stem cell secretome may also be a promising biological therapeutic option, which is equal to or even superior to the stem cell. We explored the therapeutic efficacies of ADSCs and their secretome in terms of rabbit auricular cartilage regeneration. ADSCs and their secretome were placed into surgically created auricular cartilage defects. After 4 and 8 weeks, the resected auricles were histopathologically and immunohistochemically examined. We used real-time PCR to determine the levels of genes expressing collagen type II, transforming growth factor-β1 (TGF-β1), and insulin-like growth factor-1 (IGF-1). ADSCs significantly improved auricular cartilage regeneration at 4 and 8 weeks, compared to the secretome and PBS groups, as revealed by gross examination, histopathologically and immunohistochemically. ADSCs upregulated the expression of collagen type II, TGF-β1, and IGF-1 more so than did the secretome or PBS. The expression levels of collagen type II and IGF-1 were significantly higher at 8 weeks than at 4 weeks after ADSC injection. Although ADSCs thus significantly enhanced new cartilage formation, their secretome did not. Therefore, ADSCs may be more effective than their secretome in the repair of auricular cartilage defect.

scholarly journals THE EFFECT OF DECELLULARIZATION TECHNIQUE ON COLLAGEN TYPE II AND MATRICES POROSITY OF CARTILAGE BOVINE SCAFFOLD

2019 ◽  
Vol 7 (1) ◽  
pp. 42
Author(s):  
Dwikora Novembri Utomo ◽  
Ika Benny Kartika Sari
Keyword(s):  
Stem Cell ◽  
Collagen Type ◽  
Large Diameter ◽  
Alternative Therapy ◽  
Immunohistochemical Method ◽  
Laboratory Research ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
High Concentrations

Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell.Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold.Methods: This experimental laboratory research used cartilage bovine scaffold which was decellularized using physical, chemical and enzymatic techniques with different time and concentration variations, then collagen type II measurement was performed by immunohistochemical method and evaluation of porosity size with electron microscopy.Results: In the preparations with the highest concentration and the longest immersion time of 5% SDS for 72 hours, the decellularization process occurs perfectly. The collagen type II content was found to have significantly higher concentrations than the controls and other preparations, and have interconnected porosities in large size.Conclusion: High concentrations of collagen type II  and large diameter of pores after decellularization process in cartilage bovine scaffold confirm that this scaffold still has optimum biochemical cues that can be a good mechanical framework and microenvironment stem cell in effort to repair the cartilage defects. This result was expected to become a standard for the decellularization making of cartilage bovine scaffold.

scholarly journals Serum Collagen Type II Cleavage Epitope and Serum Hyaluronic Acid as Biomarkers for Treatment Monitoring of Dogs with Hip Osteoarthritis

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149472 ◽  
Author(s):  
José M. Vilar ◽  
Mónica Rubio ◽  
Giuseppe Spinella ◽  
Belén Cuervo ◽  
Joaquín Sopena ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Collagen Type ◽  
Hip Osteoarthritis ◽  
Treatment Monitoring ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Serum Collagen

scholarly journals Effect of an oral preparation containing hyaluronic acid, chondroitin sulfate, hydrolyzed collagen type II and hydrolyzed keratin on synovial fluid features and clinical indices in knee osteoarthritis. A pilot study

Reumatismo ◽  
2020 ◽  
Vol 72 (3) ◽  
pp. 125-130
Author(s):  
F. Oliviero ◽  
R. Ramonda ◽  
A. Hoxha ◽  
A. Scanu ◽  
P. Galozzi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Pilot Study ◽  
Collagen Type ◽  
Control Group ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Knee Oa ◽  
Hydrolyzed Collagen ◽  
Oral Preparation ◽  
Clinical Indices

The aim of this study was to evaluate the effect of an oral preparation containing a naturally occurring matrix of hydrolyzed collagen type II, chondroitin sulfate (CS), and hyaluronic acid (HA), and bioactive oligopeptides of natural hydrolyzed keratin (K) in patients affected by knee OA through the evaluation of synovial fluid (SF) and clinical changes before and after treatment. Thirty patients with knee OA and swollen joint were included in the study and submitted to arthrocentesis. Patients were randomized in two groups: 1) the treatment group (N.15) took a dietary supplement containing 120 mg HA, 240 mg CS and 300 mg K once a day for 4 weeks; 2) the control group (N.15) was only submitted to arthrocentesis. Patient symptoms were evaluated at the beginning and at the end of the study by the WOMAC self-assessment questionnaire, the Lequesne algofunctional index, and the VAS forms. SF changes were evaluated by measuring local inflammatory indices, cytokines IL-1β, IL-8, IL-6, IL-10 and GM-CSF. The group of patients treated with the oral supplement showed an improvement in the clinical indices WOMAC (p<0.01), Lequesne (p=0.014) and VAS pain (p<0.01). On the contrary, no significant changes were found in the control group. The SF collected from the treated group showed a reduction of IL-8 (p=0.015), IL-6 and IL-10 levels, while no changes in cytokines were observed in the control group. This pilot study suggests that an oral administration of a preparation containing a combination of HA, CS and K can improve some clinical parameters and affect cytokine concentrations in SF in patients with knee OA.

MRI and histologic analysis of collagen type II sponge on repairing the cartilage defects of rabbit knee joints

2010 ◽  
Vol 96B (2) ◽  
pp. 267-275 ◽  
Author(s):  
Honghui Chen ◽  
Xiaohong Yang ◽  
Yingyang Liao ◽  
Xuwen Zeng ◽  
Peihong Liang ◽  
...  
Keyword(s):  
Collagen Type ◽  
Knee Joints ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Histologic Analysis ◽  
Rabbit Knee

scholarly journals Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2750 ◽  
Author(s):  
Tze-Wen Chung ◽  
Weng-Pin Chen ◽  
Pei-Wen Tai ◽  
Hsin-Yu Lo ◽  
Ting-Ya Wu
Keyword(s):  
Hyaluronic Acid ◽  
Nucleus Pulposus ◽  
Silk Fibroin ◽  
Collagen Type ◽  
Glycidyl Ether ◽  
Weight Ratio ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Viscoelastic Modulus

Silk fibroin (SF) and hyaluronic acid (HA) were crosslinked by horseradish peroxidase (HRP)/H2O2, and 1,4-Butanediol di-glycidyl ether (BDDE), respectively, to produce HA/SF-IPN (interpenetration network) (HS-IPN) hydrogels. HS-IPN hydrogels consisted of a SF strain with a high content of tyrosine (e.g., strain A) increased viscoelastic modules compared with those with low contents (e.g., strain B and C). Increasing the quantities of SF in HS-IPN hydrogels (e.g., HS7-IPN hydrogels with weight ratio of HA/SF, 5:7) increased viscoelastic modules of the hydrogels. In addition, the mean pores size of scaffolds of the model hydrogels were around 38.96 ± 5.05 μm which was between those of scaffolds H and S hydrogels. Since the viscoelastic modulus of the HS7-IPN hydrogel were similar to those of human nucleus pulposus (NP), it was chosen as the model hydrogel for examining the differentiation of human bone marrow-derived mesenchymal stem cell (hBMSC) to NP. The differentiation of hBMSC induced by transforming growth factor β3 (TGF-β3) in the model hydrogels to NP cells for 7 d significantly enhanced the expressions of glycosaminoglycan (GAG) and collagen type II, and gene expressions of aggrecan and collagen type II while decreased collagen type I compared with those in cultural wells. In summary, the model hydrogels consisted of SF of strain A, and high concentrations of SF showed the highest viscoelastic modulus than those of others produced in this study, and the model hydrogels promoted the differentiation of hBMSC to NP cells.

scholarly journals Collagen Type I and Type II Expression Evaluation on Cartilage Defect Regeneration Treated with Dwikora–Ferdiansyah–Lesmono–Purwati (DFLP) Scaffold Supplemented with Adipose–Derived Stem Cells (ASCs) or Secretome: An In-Vivo Study

2020 ◽  
Vol 4 (2) ◽  
pp. 225
Author(s):  
Adrianto Prasetyo Perbowo ◽  
Dwikora Novembri Utomo ◽  
Lukas Widhiyanto ◽  
Primadenny Ariesa Airlangga ◽  
Purwati Purwati
Keyword(s):  
Stem Cells ◽  
Collagen Type ◽  
Collagen Type I ◽  
Cartilage Defects ◽  
Type I ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Significant Difference

Abstract Cell-based therapies such as Scaffold, stem cells, and secretome, are one of the alternatives to enhance the regeneration of hyaline-like cartilage in cases of cartilage defects. This study is an in-vivo experiment using animal models, in which we apply a composite of DFLP (Dwikora-Ferdiansyah-Lesmono-Purwati) Scaffold and Adipose-Derived Stem Cells (ASCs) or Secretome to an injury model on the distal femoral trochlea of New Zealand White Rabbits. The animals were divided into four groups: (1) control (K); (2) Scaffold only (S); (3) Scaffold + ASCs (SA); (4) Scaffold + Secretome (SS). Animals were terminated in the 12th week, and an immunohistochemistry (IHC) evaluation for Collagen type I and II were done. Statistical analysis shows that collagen type I IHC between groups shows no significant difference (p = 0.546). Collagen type II IHC shows significant difference between groups (p = 0,016). The findings in this study showed that Scaffold + ASCs group and Scaffold + Secretome have better collagen type II expression compared to the control group. DFLP Scaffold composite with ASCs or Secretome shows potential for cartilage regeneration therapy by increasing type II collagen expression as in hyaline-like cartilage which may be used for regenerative therapy for cartilage defects. Keywords             : DFLP Scaffold; Adipose-Derived Stem Cells (ASCs); Secretome; Collagen Type I; Collagen Type IICorrespondence    : [email protected]

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