Tissue-Specific Metabolite Profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by Mass spectrometry imaging and Laser Microdissection Combined with UPLC-Q/TOF-MS

2021 ◽  
pp. 114070
Author(s):  
Yujiao Zhao ◽  
Shanshan Chu ◽  
Shuangying Gui ◽  
Yuejian Qin ◽  
Rui Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Metabolite Profiling ◽  
Mass Spectrometry Imaging ◽  
Laser Microdissection ◽  
Tissue Specific ◽  
Tof Ms

scholarly journals Metabolite Fingerprinting of Eugenia jambolana Fruit Pulp Extracts using NMR, HPLC-PDA-MS, GC-MS, MALDI-TOF-MS and ESI-MS/MS Spectrometry

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Ram Jee Sharma ◽  
Ramesh C. Gupta ◽  
Arvind Kumar Bansal ◽  
Inder Pal Singh
Keyword(s):  
Mass Spectrometry ◽  
Metabolite Profiling ◽  
Stem Bark ◽  
Fruit Pulp ◽  
Hyphenated Techniques ◽  
Metabolite Fingerprinting ◽  
Maldi Tof ◽  
Methanolic Extracts ◽  
Eugenia Jambolana ◽  
Tof Ms

Eugenia jambolana, commonly known as ‘jamun’ or Indian blackberry, is an important source of bioactive compounds. All parts of the plant like stem bark, leaves, flower, fruit pulp and seeds are traditionally used for many diseases. Metabolite profiling in medicinally important plants is critical to resolve the problems associated with standardization and quality control. Metabolite profiling of the fruit pulp of Jamun was performed by NMR, HPLC, MS, GC-MS and MALDI-TOF mass spectrometry. These hyphenated techniques helped in the identification of 68 chemically-diverse metabolites of the fruit pulp. These include anthocyanins, anthocyanidins, sugars, phenolics and volatile compounds. Five extracts of fruit pulp were prepared i.e. hexane, chloroform, ethylacetate, butanol and aqueous methanolic. Twenty-five metabolites identified and quantified in the n-butanol and aqueous-methanolic extracts of ripe jamun fruit by qNMR. LC-PDA-MS and MALDI-TOF spectrometry helped in deciphering thirty-nine metabolites out of which thirteen were quantified.

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