Development of real-time PCR assays to detect cashew (Anacardium occidentale) and macadamia (Macadamia intergrifolia) residues in market analysis of processed food products

LWT ◽  
2015 ◽  
Vol 62 (1) ◽  
pp. 233-241 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Isabel González ◽  
Teresa García ◽  
Rosario Martín
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Market Analysis ◽  
Anacardium Occidentale ◽  
Processed Food ◽  
Pcr Assays

Development of three real-time PCR assays to detect peanut allergen residue in processed food products

2007 ◽  
Vol 227 (3) ◽  
pp. 857-869 ◽  
Author(s):  
Elena Scaravelli ◽  
Marcel Brohée ◽  
Rosangela Marchelli ◽  
Arjon J. van Hengel
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Processed Food ◽  
Peanut Allergen ◽  
Pcr Assays

Quantitative real-time PCR assays to detect DNA degradation in soy-based food products

2009 ◽  
Vol 89 (7) ◽  
pp. 1137-1144 ◽  
Author(s):  
Sarah R Murray ◽  
Ruth C Butler ◽  
Gail M Timmerman-Vaughan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Dna Degradation ◽  
Quantitative Real Time Pcr ◽  
Pcr Assays

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

Development of real-time PCR assays for the detection of lupin residues in food products

2009 ◽  
Vol 230 (4) ◽  
pp. 597-608 ◽  
Author(s):  
Antonio M. Gomez Galan ◽  
Marcel Brohée ◽  
Elena Scaravelli ◽  
Arjon J. van Hengel ◽  
Hubert Chassaigne
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Pcr Assays

Market analysis of food products for detection of allergenic walnut (Juglans regia) and pecan (Carya illinoinensis) by real-time PCR

2015 ◽  
Vol 177 ◽  
pp. 111-119 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Isabel González ◽  
Teresa García ◽  
Rosario Martín
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Juglans Regia ◽  
Food Products ◽  
Carya Illinoinensis ◽  
Market Analysis

Duplex real-time PCR method for the detection of sesame (Sesamum indicum) and flaxseed (Linum usitatissimum) DNA in processed food products

2015 ◽  
Vol 32 (11) ◽  
pp. 1772-1785 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Rosario Martín ◽  
Isabel González ◽  
Teresa García
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Linum Usitatissimum ◽  
Food Products ◽  
Sesamum Indicum ◽  
Processed Food ◽  
Pcr Method

DEVELOPMENT OF MULTIPLEX REAL-TIME PCR ASSAY FOR SIX PATHOGENS: RAPID TOOL FOR DIAGNOSIS OF BACTERIAL MENINGITIS

2019 ◽  
pp. 60-66
Author(s):  
Viet Quynh Tram Ngo ◽  
Thi Ti Na Nguyen ◽  
Hoang Bach Nguyen ◽  
Thi Tuyet Ngoc Tran ◽  
Thi Nam Lien Nguyen ◽  
...  
Keyword(s):  
Bacterial Meningitis ◽  
Real Time ◽  
Real Time Pcr ◽  
Diagnostic Methods ◽  
Type B ◽  
E Coli ◽  
Pcr Assays ◽  
Set Up ◽  
Etiological Agents ◽  
Suis Serotype

Introduction: Bacterial meningitis is an acute central nervous infection with high mortality or permanent neurological sequelae if remained undiagnosed. However, traditional diagnostic methods for bacterial meningitis pose challenge in prompt and precise identification of causative agents. Aims: The present study will therefore aim to set up in-house PCR assays for diagnosis of six pathogens causing the disease including H. influenzae type b, S. pneumoniae, N. meningitidis, S. suis serotype 2, E. coli and S. aureus. Methods: inhouse PCR assays for detecting six above-mentioned bacteria were optimized after specific pairs of primers and probes collected from the reliable literature resources and then were performed for cerebrospinal fluid (CSF) samples from patients with suspected meningitis in Hue Hospitals. Results: The set of four PCR assays was developed including a multiplex real-time PCR for S. suis serotype 2, H. influenzae type b and N. meningitides; three monoplex real-time PCRs for E. coli, S. aureus and S. pneumoniae. Application of the in-house PCRs for 116 CSF samples, the results indicated that 48 (39.7%) cases were positive with S. suis serotype 2; one case was positive with H. influenzae type b; 4 cases were positive with E. coli; pneumococcal meningitis were 19 (16.4%) cases, meningitis with S. aureus and N. meningitidis were not observed in any CSF samples in this study. Conclusion: our in-house real-time PCR assays are rapid, sensitive and specific tools for routine diagnosis to detect six mentioned above meningitis etiological agents. Key words: Bacterial meningitis, etiological agents, multiplex real-time PCR

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