Ten new pentapeptides from protein hydrolysate of miiuy croaker (Miichthys miiuy) muscle: Preparation, identification, and antioxidant activity evaluation

Brewer’s spent grain (BSG) protein extracted from BSG was hydrolyzed using Alcalase to produce BSG protein hydrolysate. BSG protein hydrolysate was fractionated by ultrafiltration to obtain brown color BSG peptides. Antioxidant activity of BSG peptides was analyzeded and compared with reduced glutathione (GSH). BSG peptides exhibited 50% of scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical with concentrations less than 0.8 mg/mL, 0.6 mg/mL and 0.6 mg/mL, respectively. The reducing power of BSG peptides was 0.70 at the concentration of 2.00 mg/mL. 86.30% of the total amount of the BSG peptides purified by gel permeation chromatography was below 2000 Da. Because of its antioxidant activity, stability, nutritive value and low cost, BSG peptides exerts a possibility to use in food or cosmetic products.

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Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp

Food Chemistry ◽

10.1016/j.foodchem.2014.02.134 ◽

2014 ◽

Vol 158 ◽

pp. 385-391 ◽

Cited By ~ 39

Author(s):

Dongxiao Su ◽

Huihui Ti ◽

Ruifen Zhang ◽

Mingwei Zhang ◽

Zhengchen Wei ◽

...

Keyword(s):

Antioxidant Activity ◽

Structural Elucidation ◽

Cellular Antioxidant Activity ◽

Activity Evaluation

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Efficacy of Liquid Protein Hydrolysate from Chicken Feather by Proteus sp. on Chili Plant (Capsicum annum)

Proceedings International ◽

10.33263/proceedings21.043043 ◽

2020 ◽

Vol 2 (1) ◽

pp. 43

Keyword(s):

Antioxidant Activity ◽

Ph Value ◽

Protein Hydrolysate ◽

Nitrogen Sources ◽

Growth Effect ◽

Poultry Waste ◽

Carbon And Nitrogen ◽

Capsicum Annum ◽

Antibacterial And Antioxidant Activity ◽

Test Plants

Huge amounts of feathers are discarded as wastage, and it has always been environmentally concerned as they are difficult to destroy. Feather establishes over 90% protein, which gives it a rigid structure. Biotechnological techniques can help to degrade the feathers and use as biofertilizer. The best strategy is by utilizing keratinase producing keratinolytic microorganisms from the poultry waste to deteriorate the feathers. The poultry sample was collected at the local poultry farm. Using skimmed milk agar, enriched proteolytic bacteria were isolated, and the colony morphology assessed. The isolated bacteria were assessed for keratinolytic ability by using carbon and nitrogen sources. Liquid protein hydrolysate (LPH) was prepared and added as fertilizer to determine the growth effect on Capsicum annum. The antibacterial and antioxidant activity was assessed. The isolated Proteus sp. from the poultry waste has the ability to disintegrate the feathers completely on the 10th day. The enzymatic activity from Proteus sp. was observed increased with the presence of fructose (1.435 U/mL) and yeast extract (2.045 U/mL). The optimum temperature was at 40 °C (0.664 U/mL), pH value 7 (0.871 U/mL), and feather concentration at 1.5% (1.2 U/mL). LPH promoted the growth of Capsicum annum and increased total chlorophyll content (5.7341mg/g) in test plants. The antimicrobial activity displayed that Escherichia coli is susceptible to LPH, and also increased antioxidant activity was demonstrated in the test plants. Thus, the addition of liquid protein hydrolysate exhibited that it has the capability to aid plant development.

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Effects of pH, heat, and intestinal protease treatments on antioxidant activity of antioxidant peptides derived from protein hydrolysate of perilla seed meal

Korean Journal of Food Preservation ◽

10.11002/kjfp.2021.28.5.569 ◽

2021 ◽

Vol 28 (5) ◽

pp. 569-577

Author(s):

Ja Min Kim ◽

Kyung Young Yoon

Keyword(s):

Antioxidant Activity ◽

Protein Hydrolysate ◽

Seed Meal ◽

Antioxidant Peptides ◽

Effects Of Ph ◽

Perilla Seed

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A research on antioxidant activity evaluation of waste lignocellulosic biomass: optimization using response surface methodology

Cumhuriyet Science Journal ◽

10.17776/csj.678007 ◽

2020 ◽

Vol 41 (3) ◽

pp. 594-601

Author(s):

Bahar MERYEMOĞLU ◽

Burçak KAYA ◽

Berna NİŞ

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Lignocellulosic Biomass ◽

Activity Evaluation

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Production and characteristics of fish protein hydrolysate from parrotfish (Chlorurus sordidus) head

PeerJ ◽

10.7717/peerj.8297 ◽

2019 ◽

Vol 7 ◽

pp. e8297 ◽

Cited By ~ 1

Author(s):

Asep A. Prihanto ◽

Rahmi Nurdiani ◽

Annas D. Bagus

Keyword(s):

Antioxidant Activity ◽

Protein Hydrolysate ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Industrial Applications ◽

Control Treatment ◽

Degree Of Hydrolysis ◽

Fish Protein ◽

Fish Protein Hydrolysate ◽

High Level

Background Fish byproducts are commonly recognized as low-value resources. In order to increase the value, fish byproducts need to be converted into new products with high functionality such as fish protein hydrolysate (FPH). In this study, FPH manufactured from parrotfish (Chlorurus sordidus) heads using different pH, time and sample ratio was investigated. Methods Hydrolysis reactions were conducted under different pHs (5, 7, and 9) and over different durations (12 and 24 h). Control treatment (without pH adjustment (pH 6.4)) and 0 h hydrolsisis duration were applied. Hydrolysates were characterized with respect to proximate composition, amino acid profile, and molecular weight distribution. The antioxidant activity of the hydrolysate was also observed. Results The pH and duration of hydrolysis significantly affected (p < 0.05) the characteristics of FPH. The highest yield of hydrolysate (49.04 ± 0.90%), with a degree of hydrolysis of 30.65 ± 1.82%, was obtained at pH 9 after 24 h incubation. In addition, the FPH had high antioxidant activity (58.20 ± 0.55%), with a high level of essential amino acids. Results suggested that FPH produced using endogenous enzymes represents a promising additive for food and industrial applications.

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