Effects of Lactobacillus spp. on the phytochemical composition of juices from two varieties of Citrus sinensis L. Osbeck: ‘Tarocco’ and ‘Washington navel’

Flowering, fruit set and fruit growth of 'Washington Navel' orange fruit was monitored on 24-year-old Citrus sinensis trees on Sweet orange rootstocks that had been irrigated with either 5 or 20 mol m-3 NaCl for 5 years preceding measurements.Trees irrigated with high salinity water had reduced flowering intensities and lower rates of fruit set. This resulted in final fruit numbers for trees irrigated with 20 mol m-3 being 38% those of trees irrigated with 5 mol m-3 NaCl. Final fruit numbers were quantitatively related to canopy leaf area for both salinity treatments.Despite little difference between trees in terms of leaf area/fruit number ratio, slower rates of fruit growth were initially observed on high salinity trees. This effect was not apparent during the latter stages of fruit development. Consequently, fruit on trees irrigated with 20 mol m-3 NaCl grew to the same size as fruit on trees irrigated with 5 mol m-3 NaCl, but achieved this size at a later date. Measurements of Brix/acid ratios showed that fruit on high salinity trees reached maturity standards 25 days after fruit on low salinity trees.Unimpaired growth of fruit on high salinity trees during summer and autumn occurred, despite appreciable leaf abscission, suggesting that reserve carbohydrate was utilized for growth during this period. Twigs on high salinity trees had much reduced starch content at the time of floral differentiation in winter. Twig starch content and extent of floral differentiation varied in a similar way when examined as a function of leaf abscission. This suggests that reduced flowering and fruit set in salinized citrus trees is due to low levels of reserve starch, most of which has been utilized to support fruit growth in the absence of carbohydrate production during summer and autumn.

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GC-FID/MS Profiling of Supercritical CO2 Extracts of Peels from Citrus aurantium, C. sinensis cv. Washington navel, C. sinensis cv. Tarocco and C. sinensis cv. Doppio Sanguigno from Dubrovnik Area (Croatia)

Natural Product Communications ◽

10.1177/1934578x1501000745 ◽

2015 ◽

Vol 10 (7) ◽

pp. 1934578X1501000

Author(s):

Igor Jerković ◽

Jasmina Družić ◽

Zvonimir Marijanović ◽

Mirko Gugić ◽

Stela Jokić ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Citrus Sinensis ◽

Supercritical Co2 ◽

Geranyl Acetate ◽

Citrus Aurantium ◽

Coumarin Derivatives ◽

Linalyl Acetate ◽

Washington Navel ◽

Peel Oil

The peels of Citrus aurantium L. and Citrus sinensis Osbeck cultivars from the Dubrovnik region (south Croatia) were extracted by supercritical CO2 at 40°C and 10 MPa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. The extracts were analyzed in detail by gas chromatography and mass spectrometry (GC-FID/MS). Relevant similarities among the peel oil compositions of C. aurantium and C. sinensis cultivars were found with limonene predominance (up to 54.3%). The principal oxygenated monoterpenes were linalool (3.0%–5.9%), α-terpineol (0.7%–2.4%), linalyl acetate (0.0%–5.0%), geranyl acetate (0.0%-0.4%), ( Z)-citral (0.0%–1.8%) and ( E)-citral (0.0%–1.9%). Several sesquiterpenes were found with minor percentages. Coumarin derivatives were identified in all the samples among the relevant compounds. Isogeijerin dominated in the peels of C. sinensis cv. Tarocco (15.3%) and C. aurantium (11.2%). Scoparone ranged from 0.1% to 0.5% in all the samples. Bergapten (up to 1.4%), osthole (up to 1.1%) and 7-methoxy-8-(2-formylpropyl)coumarin (up to 1.1%) were found mostly in C. sinensis cv. Doppio Sanguigno. It was possible to indicate a few other differences among the extracts such as higher percentage of linalool, linalyl and geranyl acetates, as well as the abundance of sabinene and isogeijerin in C. aurantium or the occurrence of β-sinensal in C. sinensis cultivars.

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Phytochemical composition and in vitro antioxidant activities of Citrus sinensis peel extracts

PeerJ ◽

10.7717/peerj.5331 ◽

2018 ◽

Vol 6 ◽

pp. e5331 ◽

Cited By ~ 9

Author(s):

Sok Sian Liew ◽

Wan Yong Ho ◽

Swee Keong Yeap ◽

Shaiful Adzni Bin Sharifudin

Keyword(s):

Liquid Chromatography ◽

Organic Acids ◽

Citrus Sinensis ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Flavonoid Content ◽

Phytochemical Composition ◽

Acetone Water

Background Citrus sinensis peels are usually discarded as wastes; however, they are rich sources of Vitamin C, fibre, and many nutrients, including phenolics and flavonoids which are also good antioxidant agents. This study aimed to examine phytochemical composition and antioxidant capabilities of C. sinensis peel extracted conventionally with different methanol/water, ethanol/water, and acetone/water solvents. Methods C. sinensis peels were subjected to extraction with 100%, 70% and 50% of methanol, ethanol, and acetone, respectively, as well as hot water extraction. Antioxidant activities of the peel extracts were examined via the 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, and oxygen radical absorbance capacity (ORAC) assay. Total phenolic content and total flavonoid content of the extracts were measured via the Folin-Ciocalteau method and the aluminium chloride colorimetric method, respectively. Phenolic acid and organic acid composition of the peel extracts were further determined via high performance liquid chromatography (HPLC) while flavonoid content was identified via ultra performance liquid chromatography (UPLC). Results DPPH radical scavenging activity of C. sinensis peel extracts varied from 8.35 to 18.20 mg TE/g, FRAP ranged from 95.00 to 296.61 mmol Fe(II)/g, while ORAC value ranged from 0.31 to 0.92 mol TE/g. Significant level of association between the assays was observed especially between TPC and FRAP (R-square = 0.95, P < 0.0001). TPC of various C. sinensis peel extracts ranged from 12.08 to 38.24 mg GAE/g, with 70% acetone/water extract (AEC) showing the highest TPC. TFC ranged from 1.90 to 5.51 mg CE/g. Extraction yield ranged from 0.33 to 0.54 g/g DW and tended to increase with increasing water concentration in the solvent. In the phytochemical investigation, five phenolic acids were identified using HPLC, including gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, caffeic acid and ferulic acid. A total of five organic acids including lactic acid, citric acid, L-mallic acid, kojic acid and ascorbic acid were quantified via HPLC. In addition, concentrations of six flavonoids including catechin, epigallocatechin, vitexin, rutin, luteolin and apigenin were determined via UPLC. Discussion and Conclusion Phytochemicals including phenolics and flavonoids in C. sinensis peel extracts exhibited good antioxidant properties. Among the extracts, 70% AEC with highest TPC and high TFC content showed greatest antioxidant activity in all three assays. Different phenolic acids, organic acids and flavonoids were also identified from the extracts. This study indicated that C. sinensis peels contained potential antioxidant compounds which could be exploited as value added products in the food industry.

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Response of orchard 'Washington Navel' orange, Citrus sinensis (L.) Osbeck, to saline irrigation water. I. Canopy characteristics and seasonal patterns in leaf osmotic potential, carbohydrates and ion concentrations

Australian Journal of Agricultural Research ◽

10.1071/ar9890359 ◽

1989 ◽

Vol 40 (2) ◽

pp. 359 ◽

Cited By ~ 16

Author(s):

J Lloyd ◽

H Howie

Keyword(s):

Leaf Area ◽

Irrigation Water ◽

Osmotic Potential ◽

Citrus Sinensis ◽

Soluble Sugars ◽

Seasonal Patterns ◽

Navel Orange ◽

Washington Navel ◽

Leaf Osmotic Potential ◽

Chloride Concentrations

Effects of irrigation water salinity on tree canopy volume, leaf area, rates of leaf abscission and production, as well as seasonal patterns in leaf osmotic potential (=), starch, soluble sugars and sodium and chloride concentrations were determined for 24-year-old 'Washington Navel' orange trees (Citrus sinensis [L.] Osbeck) on sweet orange (C. sinensis) rootstock. Trees had been irrigated with water containing either 5 or 20 mol NaCl m-3 for 5 years prior to measurements.Trees irrigated with 20 mol NaCl m-3 had a greater number of vegetative flushes in spring. This occurred at the expense of flowering, as numbers of reproductive and mixed flushes were reduced by salinity. Despite a high number of vegetative buds on trees irrigated with 20 mol NaCl m-3, leaf area was still less than low salinity trees.Extensive abscission of spring flush leaves occurred from mid-summer onwards for trees irrigated with 20 mol NaCl m-3. This was not a consequence of leaf water deficit, as more negative leaf osmotic potentials resulting from increased foliar sodium and chloride concentrations resulted in maintenance of leaf turgor. Excessive concentrations of sodium and/or chloride may have been responsible for abscission observed. Some acclimation of foliage to salinity was evident.Irrespective of salinity treatment, leaf osmotic potential became more negative as the season progressed. This was partly due to increased concentrations of soluble sugars in foliage during autumn and winter. Levels of soluble sugars and starch were consistently lower in leaves on trees irrigated with high salinity water, indicating that production rather than utilization of carbohydrate may limit citrus productivity under saline conditions.

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