Preparation of l-α-glyceryl phosphorylcholine by hydrolysis of soy lecithin using phospholipase A1 in a novel solvent-free water in oil system

LWT ◽  
2020 ◽  
Vol 129 ◽  
pp. 109562
Author(s):  
Zi-zhe Cai ◽  
Hong-fei Wang ◽  
Wan-zhen Li ◽  
Wan Jun Lee ◽  
Wen Li ◽  
...  
Keyword(s):  
Free Water ◽  
Solvent Free ◽  
Phospholipase A1 ◽  
Soy Lecithin ◽  
Hydrolysis Of

Solvent-free enzymatic hydrolysis of non-polar lipids in crude sunflower lecithin using phospholipase A1 (Lecitase® Ultra)

2017 ◽  
Vol 36 (5) ◽  
pp. 341-351 ◽  
Author(s):  
María Laura Goñi ◽  
Consuelo Pacheco ◽  
Diana Teresita Constenla ◽  
Amalia Antonia Carelli
Keyword(s):  
Enzymatic Hydrolysis ◽  
Solvent Free ◽  
Polar Lipids ◽  
Phospholipase A1 ◽  
Hydrolysis Of

scholarly journals Evidence for the regulation of guinea-pig heart microsomal phosphatidylcholine-hydrolysing phospholipase A1 by guanosine 5′-[γ-thio]triphosphate

1992 ◽  
Vol 288 (3) ◽  
pp. 965-968 ◽  
Author(s):  
K Badiani ◽  
X Lu ◽  
G Arthur
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Guinea Pig ◽  
Molecular Species ◽  
Inhibitory Effect ◽  
Phospholipase A1 ◽  
Guinea Pig Heart ◽  
Substrate Specificities ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

We have recently characterized lysophospholipase A2 activities in guinea-pig heart microsomes and postulated that these enzymes act sequentially with phospholipases A1 to release fatty acids selectively from phosphatidylcholine (PC) and phosphatidylethanolamine, thus providing an alternative route to the phospholipase A2 mode of release. In a further investigation of the postulated pathway, we have characterized the PC-hydrolysing phospholipase A1 in guinea-pig heart microsomes. Our results show that the enzyme may have a preference for substrates with C16:0 over C18:0 at the sn-1 position. In addition, although the enzyme cleaves the sn-1 fatty acid, the rate of hydrolysis of PC substrates with C16:0 at the sn-1 position was influenced by the nature of the fatty acid at the sn-2 position. The order of decreasing preference was C18:2 > C20:4 = C18:1 > C16:0. The hydrolyses of the molecular species were differentially affected by heating at 60 degrees C. An investigation into the effect of nucleotides on the activity of the enzyme showed that guanosine 5′-[gamma-thio]triphosphate (GTP[S]) inhibited the hydrolysis of PC by phospholipase A1 activity, whereas GTP, guanosine 5′-[beta-thio]diphosphate (GDP[S]), GDP, ATP and adenosine 5′-[gamma-thio]triphosphate (ATP[S]) did not affect the activity. The inhibitory effect of GTP[S] on phospholipase A1 activity was blocked by preincubation with GDP[S]. A differential effect of GTP[S] on the hydrolysis of different molecular species was also observed. Taken together, the results of this study suggest the presence of more than one phospholipase A1 in the microsomes with different substrate specificities, which act sequentially with lysophospholipase A2 to release linoleic or arachidonic acid selectively from PC under resting conditions. Upon stimulation and activation of the G-protein, the release of fatty acids would be inhibited.

Hydrolysis of plasmalogen by phospholipase A1 from Streptomyces albidoflavus for early detection of dementia and arteriosclerosis

2015 ◽  
Vol 38 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Shin-ich Sakasegawa ◽  
Ryota Maeba ◽  
Kazutaka Murayama ◽  
Hideyuki Matsumoto ◽  
Daisuke Sugimori
Keyword(s):  
Early Detection ◽  
Phospholipase A1 ◽  
Streptomyces Albidoflavus ◽  
Hydrolysis Of

scholarly journals Preparation of Al/Fe-PILC clay catalysts from concentrated precursors: enhanced hydrolysis of pillaring metals and intercalation

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40450-40460
Author(s):  
Carlos Andrés Vallejo ◽  
Luis Alejandro Galeano ◽  
Raquel Trujillano ◽  
Miguel Ángel Vicente ◽  
Antonio Gil
Keyword(s):  
Solvent Free ◽  
Peroxide Oxidation ◽  
Highly Active ◽  
Wet Peroxide Oxidation ◽  
Catalytic Wet Peroxide Oxidation ◽  
Hydrolysis Of ◽  
Clay Catalysts

Significantly intensified preparation of Al/Fe-hydrolysed-pillaring solutions and solvent-free intercalation of bentonites yielding Al/Fe-PILCs highly active in catalytic wet peroxide oxidation.

Storage Stability of Solvent-Free Condensates of Functionalized Trialkoxysilanes

1999 ◽  
Vol 576 ◽  
Author(s):  
S. Stein ◽  
St. Cramer V. Clausbruch ◽  
N. Moszner ◽  
V. Rheinberger Ivoclar Ag
Keyword(s):  
Methyl Ether ◽  
Storage Stability ◽  
Solvent Free ◽  
Residual Silanol Groups ◽  
Acid Catalyzed ◽  
Chemical Conditions ◽  
Degree Of Condensation ◽  
Hydrolysis Of ◽  
Initial Viscosity ◽  
Butyl Methyl Ether

ABSTRACTThe lack of storage stability of organic-inorganic solvent-free sols is a well-known problem. We investigated the influence of different solvents in the acid catalyzed hydrolysis of various sols on their storage stability. Our experiments were based on two different silanes, 3-methacryloxypropyltrimethoxysilane (MPTS) and 1,1,1-tris(allyloxymethyl)-l-[[[3-(triethoxysilyl)propyl]aminocarbonyl]oxymethyl]methane (silane 31), synthesized by reaction of pentaerythritol triallyl ether and 3-isocyanatopropyltriethoxysilane. The employed solvents were alcohol, tetrahydofuran and tert-butyl methyl ether in a range of 95 wt% to 30 wt% in solution. In order to get comparable chemical conditions the degree of condensation of the various sols were adjusted between 75% and 85%. Furthermore, the sols were modified by silylation with trimethylchlorosilane. A comparison of silylated and not silylated silane 31 clearly showed that a decrease in residual silanol groups increase the storage stability. The results of the investigations demonstrated that both the kind and the amount of solvent used in hydrolysis affects the initial viscosity of solvent-free condensates of silane 31 and MPTS.

scholarly journals The effect of ultrasound on lipase-catalyzed hydrolysis of soy oil in solvent-free system

2008 ◽  
Vol 15 (4) ◽  
pp. 402-407 ◽  
Author(s):  
Yaxuan Liu ◽  
Qingzhe Jin ◽  
Liang Shan ◽  
Yuanfa Liu ◽  
Wei Shen ◽  
...  
Keyword(s):  
Solvent Free ◽  
Soy Oil ◽  
Free System ◽  
Solvent Free System ◽  
Hydrolysis Of

Hydrolysis of amides to carboxylic acids using phthalic anhydride under microwave irradiation and solvent-free conditions

2005 ◽  
Vol 2005 (2) ◽  
pp. 119-120 ◽  
Author(s):  
Majid M. Heravi ◽  
Dordaneh Zargarani ◽  
Rahim Hekmat Shoar ◽  
Shahnaz Khaleghi
Keyword(s):  
Microwave Irradiation ◽  
Carboxylic Acids ◽  
Phthalic Anhydride ◽  
Solvent Free ◽  
Solvent Free Conditions ◽  
High Yields ◽  
Hydrolysis Of

Amides efficiently and rapidly give carboxylic acids in high yields upon reaction with phthalic anhydride under microwave irradiation in the absence of solvent.

Free-fatty acid profile obtained by enzymatic solvent-free hydrolysis of sunflower and soybean lecithins

2010 ◽  
Vol 120 (1) ◽  
pp. 332-338 ◽  
Author(s):  
María C. Penci ◽  
Diana T. Constenla ◽  
Amalia A. Carelli
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fatty Acid Profile ◽  
Solvent Free ◽  
Hydrolysis Of

scholarly journals Studies of the processes operative in solutions. XXIII.—The hydrolysis of methylic acetate by acids

1912 ◽  
Vol 87 (599) ◽  
pp. 582-603
Keyword(s):  
Simple Formula ◽  
Free Water ◽  
Acid Catalyst ◽  
Cane Sugar ◽  
Rate Of Change ◽  
Active Factor ◽  
Primary Object ◽  
Rate Of Hydrolysis ◽  
Extreme Care ◽  
Hydrolysis Of

One of the main objects in view throughout this series of studies of the processes operative in solutions has been to discover the nature of the changes which attend dissolution, particularly the extent to which substances undergo hydration when dissolved in water; another primary object has been to unravel the nature of the process of hydrolysis. The evidence thus far obtained has been always such as to show that the phenomena are far more complex than is usually supposed and even to discredit the interpretation that it is now customary to give of them. In Communication XII of the series, I have given an account of experiments made with improved apparatus and with extreme care of the behaviour of cane sugar towards chlorhydric and nitric acids and of the influence of certain chlorides and nitrates on the rate of change. It was shown that the rate of hydrolysis could not be accurately expressed by any simple formula, as it was not even proportional to the concentration of the sugar, though it was approximately so when the molecular ratio of sugar to water was not high. Evidence was adduced to show that water as such, i.e. free water, did not play any part in the change but acted solely as solvent and that the active factor of the interaction besides the sugar was the hydrated acid catalyst.

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