Antioxidant and cytoprotective effects of synthetic peptides identified from Kluyveromyces marxianus protein hydrolysate: insight into the molecular mechanism

LWT ◽  
2021 ◽  
pp. 111792
Author(s):  
Saeed Mirdamadi ◽  
Mahta Mirzaei ◽  
Nazila Soleymanzadeh ◽  
Maliheh Safavi ◽  
Nahid Bakhtiari ◽  
...  
Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 342 ◽  
Author(s):  
Ida Di Mola ◽  
Stefano Conti ◽  
Eugenio Cozzolino ◽  
Giuseppe Melchionna ◽  
Lucia Ottaiano ◽  
...  

Hemp (Cannabis sativa L.) is a multipurpose plant attracting increasing interest as a source for the production of natural fibers, paper, bio-building material and food. In this research we studied the agronomical performance of Cannabis sativa cv. Eletta Campana irrigated with saline water. Under those conditions, we tested the effect of protein hydrolysate (PH) biostimulant application in overcoming and/or balancing deleterious salinity effects. The results of the diverse treatments were also investigated at the physiological level, focusing on photosynthesis by means of a chlorophyll a fluorescence technique, which give an insight into the plant primary photochemical reactions. Four salinity levels of the irrigation solution (fresh water–EC0, and NaCl solutions at EC 2.0, 4.0 or 6.0 dS m−1, EC2, EC4 and EC6, respectively) were combined with 2 biostimulant treatments (untreated (control) or treated with a commercial legume-derived protein hydrolysate (LDPH)). The increasing salinity affected plant photochemistry resulting in lower plant growth and seed production, while the LDPH biostimulant showed a protective effect, which improved crop performance both in control and in salinity conditions. The LDPH treatment improved seeds yield (+38.6% on average of all treated plants respect to untreated plants), as well as residual biomass, relevant in fiber production.


2015 ◽  
Vol 173 ◽  
pp. 91-99 ◽  
Author(s):  
Yi-min Ma ◽  
Xin-zhuang Zhang ◽  
Zhen-zhen Su ◽  
Na Li ◽  
Liang Cao ◽  
...  

2015 ◽  
Vol 113 ◽  
pp. 127-142 ◽  
Author(s):  
Rekha Jain ◽  
Prajakta Kulkarni ◽  
Snigdha Dhali ◽  
Srikanth Rapole ◽  
Sanjeeva Srivastava

2010 ◽  
Vol 29 (20) ◽  
pp. 3437-3447 ◽  
Author(s):  
Carsten Peters ◽  
Katjuša Brejc ◽  
Lisa Belmont ◽  
Andrew J Bodey ◽  
Yan Lee ◽  
...  

2021 ◽  
Author(s):  
Liheng Chen ◽  
Si-Man Luo ◽  
Cong-Min Huo ◽  
Yun-Feng Shi ◽  
Jun Feng ◽  
...  

Understanding the molecular mechanism of lignin nanoparticle (LNP) formation will precisely instruct its functionalization, which is of importance for biomass valorization.


Author(s):  
Akhilesh Kumar Singh ◽  
Laxuman Sharma ◽  
Janmejai Kumar Srivastava ◽  
Nirupama Mallick ◽  
Mohammad Israil Ansari

Blood ◽  
1995 ◽  
Vol 86 (10) ◽  
pp. 3789-3796 ◽  
Author(s):  
R He ◽  
DM Reid ◽  
CE Jones ◽  
NR Shulman

Glycoproteins (GPs) IIb/IIIa and Ib/IX are principal targets of autoantibodies (autoAbs) in idiopathic thrombocytopenic purpura (ITP). Platelet-associated Abs against GPIIb/IIIa primarily recognize discontinuous or nonlinear epitopes (Fujisawa et al, Blood 81:1284, 1993). This study focused on whether Abs against the extracellular domain of GPIb/IX might react with short linear amino acid (aa) sequences of GPIb alpha. Complementary DNAs (cDNAs) coding for two overlapping fragments of GPIb alpha were amplified, cloned into pFLAG.2 plasmids, and expressed in Escherichia coli DH5 alpha competent cells as FLAG fusion proteins, which were purified by anti-FLAG immunoaffinity chromatography. Of 16 selected ITP sera containing anti- GPIb/IX, 6 reacted in microtiter radioimmunoassays (RIAs) with recombinant protein fragment 2 (aas 240 to 485); 1 also with fragment 1 (aas 1 to 247). When synthetic peptides corresponding to 4 segments of fragment 2 with high antigenic indices (P1 to P4) were used as targets in RIAs, all 6 sera reacted with P2 (aas 326 to 346); 1 also reacted with P4 (aas 389 to 412). P2 was shown to be present on the surface of intact platelets by adsorption studies, and anti-P2 was detected in direct eluates of platelets from ITP patients. Glycocalicin in solution effectively competed with immobilized P2 for anti-P2; P2 in solution was a less effective competitor. Epitope scanning with a panel of synthetic 15-mer peptides localized the P2 epitope to the sequence, TKEQTTFPP. Epitope definition may offer insight into the pathophysiology of and more specific treatments for ITP.


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