Closure times of neurocranial sutures and synchondroses in Persian compared to Domestic Shorthair cats

Human-directed selective breeding has modified the phenotype of the modern Persian cat towards an extreme brachycephalic phenotype (‘peke-face’ Persian), which originates from a spontaneous mutation that first appeared in the 1950s in traditional Persian types. It was suggested that the peke-face phenotype results from pathologic skull development and might represent a craniosynostosis of the coronal sutures. We followed this hypothesis and investigated the time dependent status of the neurocranial sutures and synchondroses in an ontogenetic series of doll-faced and peke-faced Persian cats compared to Domestic Shorthair cats (DSHs). Cranial suture closure was assessed by examining an ontogenetic series of formalin-fixed head specimens (n = 55) and dry skulls (n = 32) using micro-computed tomography. Sagittal, metopic, coronal and lambdoid sutures as well as intersphenoidal, spheno-occipital and spheno-ethmoid synchondroses were examined. Logistic regression analysis was performed to test the global effect of age on suture closure within a group of peke-face Persians, doll-face Persians and DSHs and the 50% probability of having a closed suture was calculated and compared between groups. Age was a perfect predictor for the condition of the coronal sutures in peke-face Persians. Coronal sutures were found to be closed at 0–0.3 months. In doll-face and DSHs, coronal sutures were open throughout the lifetime with the exception of a few very old cats. Results of this study confirmed a coronal craniosynostosis that likely causes the extreme brachycephalic skull morphology in the peke-face Persian.

Rotavirus RNA chaperone mediates global transcriptome-wide increase in RNA backbone flexibility

Due to genome segmentation, rotaviruses must co-package a set of eleven distinct genomic RNAs. The packaging is mediated by virus-encoded RNA chaperones, such as the rotavirus (RV) NSP2 protein. While the activities of distinct viral RNA chaperones are well studied on synthetic RNA substrates, little is known about their global effect on the entire viral transcriptome. Here we used Selective 2′-hydroxyl Acylation Analyzed by Primer Extension and Mutational Profiling (SHAPE-MaP) to systematically examine the secondary structure of the RV transcriptome composed of eleven distinct transcripts in the absence and presence of increasing concentrations of RV NSP2. Surprisingly, SHAPE-MaP data reveals that despite the well-documented helix-unwinding activity of NSP2 in vitro, its incubation with cognate RV transcripts does not induce a significant change in the SHAPE reactivities. However, a quantitative analysis of the per nucleotide mutation rate measured by mutational profiling, from which SHAPE reactivities are derived, reveals a global five-fold rate increase in the presence of molar excess of NSP2. We demonstrate that the standard normalization procedure used in deriving SHAPE reactivities from mutation rates can mask an important global effect of an RNA chaperone activity. Further analysis of the mutation rate in the context of structural classification reveals a larger effect on stems rather than loop elements. Together, these data provide the first experimentally derived secondary structure model of the RV transcriptome and reveal that NSP2 acts by globally increasing RNA backbone flexibility in a concentration-dependent manner, consistent with its promiscuous RNA-binding nature.

Effect of SARS-CoV-2 Vaccination on Symptoms from Post-Acute Sequelae of COVID-19: Results from the Nationwide VAXILONG Study

Introduction: Few data are available concerning the effect of SARS-CoV-2 vaccination on the persistent symptoms associated with COVID-19, also called long-COVID or post-acute sequelae of COVID-19 (PASC). Patients and methods: We conducted a nationwide online study among adult patients with PASC as defined by symptoms persisting over 4 weeks following a confirmed or probable COVID-19, without any identified alternative diagnosis. Information concerning PASC symptoms, vaccine type and scheme and its effect on PASC symptoms were studied. Results: 620 questionnaires were completed and 567 satisfied the inclusion criteria and were analyzed. The respondents’ median age was 44 (IQR 25–75: 37–50) and 83.4% were women. The initial infection was proven in 365 patients (64%) and 5.1% had been hospitalized to receive oxygen. A total of 396 patients had received at least one injection of SARS-CoV-2 vaccine at the time of the survey, after a median of 357 (198–431) days following the initially-reported SARS-CoV-2 infection. Among the 380 patients who reported persistent symptoms at the time of SARS-CoV-2 vaccination, 201 (52.8%) reported a global effect on symptoms following the injection, corresponding to an improvement in 21.8% and a worsening in 31%. There were no differences based on the type of vaccine used. After a complete vaccination scheme, 93.3% (28/30) of initially seronegative patients reported a positive anti-SARS-CoV-2 IgG. A total of 170 PASC patients had not been vaccinated. The most common reasons for postponing the SARS-CoV-2 vaccine were fear of worsening PASC symptoms (55.9%) and the belief that vaccination was contraindicated because of PASC (15.6%). Conclusion: Our study suggests that SARS-CoV-2 vaccination is well tolerated in the majority of PASC patients and has good immunogenicity. Disseminating these reassuring data might prove crucial to increasing vaccine coverage in patients with PASC.

Changing Impacts of Tropical Cyclones on East and Southeast Asian Inland Regions in the Past and a Globally Warmed Future Climate

The impacts of the western North Pacific (WNP) tropical cyclone (TC) on East and Southeast Asian inland regions are analyzed. Here, based on a stringent TC selecting criterion, robust increase of TC-related inland impacts between 1979 and 2016 over East and Southeast Asian regions have been detected. The storms sustained for 2–9 h longer and penetrated 30–190 km further inland, as revealed from different best track datasets. The most significant increase of the TC inland impacts occurred over Hanoi and South China. The physical mechanism that affects TC-related inland impacts is shortly discussed. First, the increasing TC inland impacts just occur in the WNP region, but it is not a global effect. Second, besides the significant WNP warming effects on the enhanced TC landfall intensity and TC inland impacts, it is suggested that the weakening of the upper-level Asian Pacific teleconnection pattern since 1970s may also play an important role, which may reduce the climatic 200 hPa anti-cyclonic wind flows over the Asian region, weakening the wind shear near the Philippine Sea, and may eventually intensify the TC intensity when the TCs across the basin. Moreover, the TC inland impacts in the warming future are projected based on a high-resolution (20 km) global model according to the Representative Concentration Pathway 8.5 scenario. By the end of the 21st century, TC mean landfall intensity will increase by 2 m/s (6%). The stronger storms will sustain 4.9 h (56%) longer and penetrate 92.4 km (50%) farther inland, thereby almost doubling the destructive power delivered to Asian inland regions. More inland locations will therefore be exposed to severe storm–related hazards in the future due to warmer climate. Long-term planning to enhance disaster preparedness and resilience in these regions is called for.

Effects of arousal and movement on secondary somatosensory and visual thalamus

Neocortical sensory areas have associated primary and secondary thalamic nuclei. While primary nuclei transmit sensory information to cortex, secondary nuclei remain poorly understood. We recorded juxtasomally from secondary somatosensory (POm) and visual (LP) nuclei of awake mice while tracking whisking and pupil size. POm activity correlated with whisking, but not precise whisker kinematics. This coarse movement modulation persisted after facial paralysis and thus was not due to sensory reafference. This phenomenon also continued during optogenetic silencing of somatosensory and motor cortex and after lesion of superior colliculus, ruling out a motor efference copy mechanism. Whisking and pupil dilation were strongly correlated, possibly reflecting arousal. Indeed LP, which is not part of the whisker system, tracked whisking equally well, further indicating that POm activity does not encode whisker movement per se. The semblance of movement-related activity is likely instead a global effect of arousal on both nuclei. We conclude that secondary thalamus monitors behavioral state, rather than movement, and may exist to alter cortical activity accordingly.

EFFECTIVENESS OF DIGITAL PLATFORMS AS FOOD AND BEVERAGE MARKETING MEDIA DURING THE COVID-19 PANDEMIC

Covid-19 pandemic disrupted business practices worldwide, especially in food and everage sector. Not a few business actors in this sector closed their business due to declining sales during pandemic. In responding to this global effect, business actors are not only encouraged to adapt. Still, they must also think of practical steps to stabilize sales as before through creative and innovative digital marketing strategies. Data collected by interviews and literature study to provide an in-depth analysis of condition of food and beverage during Covid-19 pandemic and provide education about digital marketing strategies that business actors can apply to increase food and beverage sales during Covid-19 pandemic. The results showed that social media such as Instagram, Facebook, Twitter, and Whatsapps were effective in marketing food and beverage products. In addition, Shopeefood e-commerce platform and food delivery applications such as GrabFood and GoFood also play a significant role in success of food and beverage marketing and sales and can support government regulations to stay at home during the Covid-19 pandemic.

Air quality in Barcelona during the COVID-19 lockdown and the global effect on CO2 emissions

Calidad del aire en Barcelona durante el confinamiento por la COVID-19 y su efecto global en las emisiones de CO2 La pandemia de la COVID-19 está teniendo un enorme impacto negativo en la salud humana, con más de 4,44 millones de muertos en todo el mundo; en la economía, con una profunda y abrupta crisis sin precedentes; y en la sociedad, con millones de personas en situación de desempleo y dependientes, en el mejor de los casos, de los sistemas de asistencia social o de bienestar público. El confinamiento domiciliario, las medidas para limitar la actividad económica no esencial y las restricciones de los viajes en muchos países durante la primavera de 2020 llevaron a mejoras rápidas en la calidad del aire de las ciudades. Un análisis detallado de las inmisiones diarias de Barcelona de siete contaminantes (CO, SO2, NO, NO2, NOx, PM10 y O3) muestra una clara disminución desde mediados de marzo hasta junio, con el pico en abril y en los óxidos de nitrógeno. Para estos contaminantes, la reducción de la densidad del tráfico es muy probablemente la principal causa de la disminución de sus inmisiones. Por otro lado, la reducción global de las emisiones de CO2 derivadas del uso de combustibles fósiles se estimó en aproximadamente un 8 % en 2020, lo que, a pesar de constituir una caída sin precedentes, tendrá poco impacto en las concentraciones de CO2 y de otros gases de efecto invernadero en la atmósfera. La situación actual debe ser vista como una oportunidad para impulsar, sin demora, un cambio drástico en los modelos energético y económico hacia un paradigma socioambiental basado en la sostenibilidad, la eficiencia económica y la solidaridad entre los pueblos.

Processing of slow-global auditory regularities causes larger neural responses to heartbeats in patients under minimal consciousness state, compared to unresponsive wakefulness syndrome

Objectives: Determine if contextual processing of auditory regularities (local-global paradigm) modulate the heartbeat-evoked responses (HERs), and if these modulations may provide complementary information to the auditory related potentials to facilitate the diagnose of patients with disorders of consciousness (DOC). Methods: DOC patients underwent the local-global paradigm to evaluate the cognitive processing of short/long term-auditory regularities. High-density EEG was recorded to evaluate HERs in local, global and overall conditions. Results: The global effect triggers higher HERs, as well as overall higher HER variance appears in minimally conscious patients. The variance and amplitude effects were uncorrelated, indicating different cognitive processes. Discussion: Our results corroborate previous findings on HER variance, a marker able to separate DOC without specific stimulations. HER modulations in response to global auditory irregularities represents a novel neural signature of consciousness access, which may facilitate the bedside diagnosis of state of consciousness with more affordable options to neuroimaging methods.

Un(COIL)ing the Pandemic: Active and Affective Learning in Times of COVID-19

ABSTRACT The COVID-19 pandemic has had a global effect on higher education. Overnight, entire degree programs had to be moved online. Whereas this meant that teaching and learning in political science and international relations also went into “emergency e-learning” mode, as a recent teacher spotlight in PS: Political Science & Politics termed it, moving online also offered opportunities. One opportunity is collaborative online international learning (COIL) that enables students from universities in different countries to work on a common project. This article argues that working together collaboratively online not only mitigates the pandemic’s physical restrictions and sustains a global space of learning; it also provides for a particular active and affective learning in an intercultural virtual environment that substantiates classroom experiences even in post-pandemic higher education. To support this argument, this article reflects on the experiences of a British–Japanese COIL project that investigated political responses to COVID-19.

Hyperthermia-Induced Changes in EEG of Anesthetized Mice Subjected to Passive Heat Exposure

Currently, the role of hypothermia in electroencephalography (EEG) is well-established. However, few studies have investigated the effect of hyperthermia on EEG, an important physiological parameter governing brain function. The aim of this work was to determine how neuronal activity in anesthetized mice is affected when the temperature rises above the physiological threshold mandatory to maintain the normal body functions. In this study, a temperature-elevation protocol, from 37 to 42°C, was applied to four female mice of 2–3 months old while EEG was recorded simultaneously. We found that hyperthermia reduces EEG amplitude by 4.36% when rising from 37 to 38 degrees and by 24.33% when it is increased to 42 degrees. Likewise, increasing the body temperature produces a very large impact on the EEG spectral parameters, reducing the frequency power at the delta, theta, alpha, and beta bands. Our results show that hyperthermia has a global effect on the EEG, being able to change the electrical activity of the brain.