Dopamine assisted incorporation of Sr ions in porous titanium alloy and its in-vitro bioactivity and cellular responses

2021 ◽  
pp. 129308
Author(s):  
Tao Gao ◽  
Junwei Zhang ◽  
Nihui Zhang ◽  
Yao Wang ◽  
Jing He ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Cellular Responses ◽  
Porous Titanium ◽  
In Vitro Bioactivity

scholarly journals Antimicrobial properties of antibiotic-loaded implants

2020 ◽  
Vol 102-B (6_Supple_A) ◽  
pp. 158-162
Author(s):  
Quentin Griseti ◽  
Christophe Jacquet ◽  
Pierre Sautet ◽  
Matthew P. Abdel ◽  
Sébastien Parratte ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Antimicrobial Properties ◽  
Porous Metal ◽  
Porous Titanium ◽  
Inhibitory Effect ◽  
Control Group ◽  
Local Antibiotics ◽  
Significant Difference ◽  
Vitro Analysis

Aims The aim of this study was to compare the ability of tantalum, 3D porous titanium, antibiotic-loaded bone cement, and smooth titanium alloy to inhibit staphylococci in an in vitro environment, based on the evaluation of the zone of inhibition (ZOI). The hypothesis was that there would be no significant difference in the inhibition of methicillin-sensitive or methicillin-resistant Staphylococcus aureus (MSSA/MRSA) between the two groups. Methods A total of 30 beads made of three different materials (tantalum/3D porous titanium and smooth titanium alloy) were bathed for one hour in a solution of 1 g vancomycin in 20 ml of sterile water for injection (bath concentration: 50 mg/mL). Ten 1 cm3 cylinders of antibiotic-loaded cement were also created by mixing standard surgical cement with 1 g of vancomycin in standardized sterile moulds. The cylinders were then placed on agar plates inoculated with MSSA and MRSA. The ZOIs were measured each day and the cylinders were transferred onto a new inoculated plate. Results For MSSA and MRSA, no inhibitory effect was found in the control group, and antibiotic-loaded smooth titanium alloy beads showed a short inhibitory effect until day 2. For MSSA, both tantalum and 3D porous titanium beads showed significantly larger mean ZOIs than cement beads (all p < 0.01) each day until day 7 for tantalum and until day 3 for 3D porous titanium. After six days, antibiotic-loaded cement had significantly larger mean ZOIs than the 3D porous titanium (p = 0.027), but no significant difference was found with tantalum (p = 0.082). For MRSA, both tantalum and 3D porous titanium beads had significantly larger mean ZOIs than antibiotic-loaded cement each day until day 6 for tantalum (all p < 0.01) and until day 3 for 3D porous titanium (all p < 0.04). Antibiotic-loaded cement had significantly larger mean ZOIs than tantalum and 3D porous titanium from day 7 to 9 (all p < 0.042). Conclusion These results show that porous metal implants can deliver local antibiotics over slightly varying time frames based on in vitro analysis. Cite this article: Bone Joint J 2020;102-B(6 Supple A):158–162.

scholarly journals Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

2021 ◽  
Vol 9 ◽  
Author(s):  
Youbin Li ◽  
Yuzhe Liu ◽  
Haotian Bai ◽  
Ronghang Li ◽  
Jing Shang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Growth Factor ◽  
Composite Scaffold ◽  
Umbilical Vein ◽  
Tumor Resection ◽  
Bone Defects ◽  
Porous Titanium ◽  
Bone Integration

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.

Microstructure and in vitro bioactivity of laser-cladded bioceramic coating on titanium alloy in a simulated body fluid

2010 ◽  
Vol 489 (1) ◽  
pp. 211-214 ◽  
Author(s):  
Min Zheng ◽  
Ding Fan ◽  
Xiu-kun Li ◽  
Jian-bin Zhang ◽  
Qi-bin Liu
Keyword(s):  
Titanium Alloy ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
In Vitro Bioactivity

Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

2019 ◽  
Vol 35 (4) ◽  
pp. 686-692 ◽  
Author(s):  
Mengna Duan ◽  
Xiaofeng Wu ◽  
Long Yuan ◽  
Zhiying Zhang ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Titanium Implant ◽  
Porous Titanium ◽  
Hydroxyapatite Coating ◽  
In Vitro Bioactivity

scholarly journals In vitro and in vivo evaluations of mechanical properties, biocompatibility and osteogenic ability of sintered porous titanium alloy implant

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36512-36520 ◽  
Author(s):  
Ji Li ◽  
Zhongli Li ◽  
Ruiling Li ◽  
Yueyi Shi ◽  
Haoran Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Titanium Alloy ◽  
Bone Ingrowth ◽  
Porous Titanium ◽  
Porous Ti ◽  
Good Biocompatibility

The sintered porous Ti6Al4V with 75% porosity has optimal mechanical properties, good biocompatibility and osteogenic ability for more bone ingrowth.

scholarly journals MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Liu ◽  
Jinghuan Huang ◽  
Feng Chen ◽  
Dong Xie ◽  
Longqing Wang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Extracellular Vesicles ◽  
Porous Titanium ◽  
Fluorescent Labeling ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Alizarin Red ◽  
Alkaline Phosphatase Staining

Abstract Background Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading to implant failure, implant revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, and we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection and transportation of miR-20a (sEV-20a). In this study, we intended to determine whether sEVs overexpressing miR-20a could exert a superior effect on osteoporotic bone defects and the underlying mechanism. Methods For evaluating the effect of sEV-20a on osteogenesis, in vitro and in vivo studies were performed. In vitro, we first showed that miR-20a was upregulated in the osteogenic process and overexpressed sEVs with miR-20a by the transfection method. Then, the proliferation, migration, and osteogenic differentiation abilities of hBM-MSCs treated with sEV-20a were detected by CCK-8 assays, alkaline phosphatase staining and alizarin red staining, qRT-PCR, and western blot. In vivo, we established an osteoporotic bone defect model and evaluated the effect of sEV-20a on bone formation by micro-CT, sequential fluorescent labeling, and histological analysis. To further explore the mechanism, we applied a bioinformatics method to identify the potential target of miR-20a. Results In vitro, sEV-20a was successfully established and proved to promote the migration and osteogenesis of hBM-MSCs. In vivo, sEV-20a promoted osteointegration in an osteoporotic rat model. To further elucidate the related mechanism, we proved that miR-20a could enhance osteogenesis by targeting BAMBI. Conclusions Collectively, the in vitro and in vivo results confirmed that MSC-derived sEV-20a therapy effectively promoted osteoporotic porous titanium alloy osteointegration via pro-osteogenic effects by targeting BAMBI.

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