Role of purified beta-glucanase from lactobacillus acidophilus in disruption of biofilm formation by candida spp. causing of denture stomatitis

2021 ◽  
Author(s):  
Jawaher M. Taher ◽  
Noor Natik Raheem
Keyword(s):  
Lactobacillus Acidophilus ◽  
Biofilm Formation ◽  
Candida Spp ◽  
Denture Stomatitis ◽  
Beta Glucanase

scholarly journals Biofilm formation of Candida Spp. isolated from the vagina and antibiofilm activities of lactic acid bacteria on the these Candida Isolates

2020 ◽  
Vol 20 (2) ◽  
pp. 641-648
Author(s):  
Merih Kıvanç ◽  
Sevda Er
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Virulence Factors ◽  
Lactobacillus Acidophilus ◽  
Biofilm Formation ◽  
Bacterial Cells ◽  
Candida Spp ◽  
Plate Method ◽  
Microtitration Plate ◽  
Esterase Activities

Background: In this study, it was aimed to investigate the effects of bacterial cells and cell-free filtrates of Lactobacillus acidophilus 8MR7 and Lactobacillus paracasei subspecies paracasei 10MR8 on the biofilm formation of 3 Candida tropicalis, 3 C. glabrata and 12 C. albicans isolated from the vagina and identified their virulence factors. Methods: Haemolytic activities esterase activities, and phospholipase activities as virulence factors of Candida strains were determined. Biofilm formations of these isolates were determined by Congo Red agar and microtitration plate method. Anti-biofilm activities of bacterial cells and cell-free filtrates of L. acidophilus 8MR7 and L. paracasei subspecies paracasei 10MR8 on Candida isolates were determined by the microtitration plate method. Results: Bacterial cells of L. acidophilus 8MR7 and L. paracasei subspecies paracasei 10MR8 were not very effective in the in- hibition of biofilm, whereas it has been observed that the cell-free filtrates of these bacteria inhibit the formation of biofilms of Candida strains. Although the main mechanism for inhibiting the formation of Candida spp. biofilm is the competition for adhesion, it is concluded that the substances contained in the cell-free filtrates of lactic acid bacteria are also important. Conclusion: These isolates promise hope as potential bacteria that can be used for anti-adhesion purposes in health-care materials. Keywords: Lactobacillus acidophilus; L. paracesei subspecies paracesei; vagina; biofilm.

scholarly journals Role of Candida glabrata as nosocomial pathogen and its susceptibility to Fluconazole, Voriconazole, Caspofungin, Micafungin and Amphotericin B

Bionatura ◽  
2021 ◽  
Vol 3 (3) ◽  
pp. 2001-2008
Author(s):  
Teeba Hashim Mohammed ◽  
Mohsen Hashim Risan ◽  
Mohammed Kadhom ◽  
Emad Yousif
Keyword(s):  
Biofilm Formation ◽  
Candida Glabrata ◽  
Bloodstream Infections ◽  
Antifungal Drugs ◽  
Candida Spp ◽  
Minimum Fungicidal Concentration ◽  
Weak Minimum ◽  
Different Types ◽  
Chromogenic Agar

Candida has different types that could cause bloodstream infections. A total number of 150 samples were collected from candidemia patients and examined. The Candida spp. Species isolated from blood samples were analysed. These were identified by culturing the species using different media, namely the chromogenic agar test. Then, the virulence factors of all samples were tested. The Candida glabrata isolates were tested with six commercial antifungal drugs. C. glabrata 67 (44.6%), C. albicans 34 (22.6%), C. krusei 18 (12%), C. tropicalis 17 (11.3%), and C. parasilosis 14 (9.3%). the production of phospholipase ranged between 0.63-0.99 mm. It was found that 96% of the species showed phospholipase activity in aerobic conditions. The protease activities of Candida spp. Isolates were experimentally tested by area of inhibition around the colonies, where 59.3% had the double (++) protease activity, 31.4% with (+) grade, and 9.3% had (–) grade or clear zone around the colony. The hemolytic capacity ranged from 0.69-0.89 in the optimum aerobic environments. Finally, 38.33% of the isolated Candida spp. were positive and 61.67% negative for biofilm formation. Out of the total positive Candida spp. for biofilm formation, 21.73% were strong biofilm producers, and 78.27% were weak. Minimum fungicidal concentration (MFC) of Fluconazole for C. glabrata isolates was not appropriate (NA) due to the occurrence of low inhibition tested for species. Micafungin exhibited the lowest fungicidal activity against C. glabrata ranging from 0.03 - 0.125, while Fluconazole showed the highest.

scholarly journals Activity of antifungal drugs and Brazilian red and green propolis extracted with different methodologies against oral isolates of Candida spp.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ana Rita Sokolonski ◽  
Maísa Santos Fonseca ◽  
Bruna Aparecida Souza Machado ◽  
Kathleen Ramos Deegan ◽  
Roberto Paulo Correia Araújo ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Biofilm Formation ◽  
Oral Candidiasis ◽  
Extraction Procedure ◽  
Antimicrobial Activities ◽  
Antifungal Drugs ◽  
Candida Spp ◽  
Denture Stomatitis ◽  
Minimal Inhibitory Concentrations ◽  
Ultrasound Pretreatment

Abstract Background Oral candidiasis is an opportunistic disease caused by fungi of the Candida genus. The occurrence of Candida spp. resistance to the commercial antifungal drugs points to the search for alternative treatments. Propolis has been successfully used in the treatment of infectious diseases for centuries. It has been proposed that an ultrasound pretreatment in the propolis extraction protocol can enhance the concentrations of molecules with antimicrobial activities in the final extract. Thus, this study aimed to compare the antifungal activity against oral Candida spp. isolates of green and red propolis extracts submitted or not to an ultrasound pretreatment before the extraction procedure. Methods Candida spp. were isolated from denture stomatitis lesions and identified by sequencing. Oral Candida spp. isolates and reference strains were submitted to broth microdilution assays using commercial antifungals and Brazilian green and red propolis extracts submitted or not to an ultrasound pretreatment. Minimal Inhibitory Concentrations (MIC) and Minimal Fungicide Concentrations (MFC) were determined and biofilm formation interference was evaluated for resistant isolates. Results C. albicans, Candida tropicalis and Candida dubliniensis were isolated from denture stomatitis lesions. Growth inhibition was observed in all Candida isolates incubated with all green and red propolis extracts. At lower doses, red propolis extracts presented significant antifungal activity. The ultrasound pretreatment did not promote an increase in the antifungal activity of green or red propolis. Three isolates, which were highly resistant to fluconazole and itraconazole, were susceptible to low doses of red propolis extracts. These same three specimens had their biofilm formation inhibted by red propolis ethanolic extract. Conclusions Thus, red propolis can be faced as a promising natural product to be used in the auxiliary antifungal therapy of denture stomatitis.

The effect of some chemical materials against virulence factors of Candida ssp isolated from UTI patients

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Fatima Abdul Hussein Mejbel
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Germ Tube ◽  
Laboratory Culture ◽  
Candida Spp ◽  
Other Substances ◽  
Chemical Materials

 During the period from September 2016 to December 2017,135 urine samples were collected from urinary tract infection patients attending to AL-Zahraa Hospital in AL-Najaf Governorate. The present study was conducted to isolate and identify Candida spp. isolated from urinary tract infection patients by different methods including direct examination, laboratory culture, biochemical test and by modern techniques (Api Candida kit) and determine the virulence factors phenotypic to Candida spp which involved (biofilm formation,phospholipase and germ tube). The percentage of females to males was as following, female (84) 62.2 % (21) infected and male (51) 37.8% (1) infected with all age categories. The results in this study are explain that is some Candida spp. such as C. albicans, have high susceptible to eugenole follow by phenol and umbellulone. The efficiency of some chemical substances such as (eugenole,umbellulone, and phenol) was evaluated to inhibit the growth of Candida ssp as well as some virulence factors such as biofilm formation,germ tube and phospholipase,which were studied in this research. Statistically analysis results have been significance difference between the results of the substance concentrations and the concentrations of the different other substances.

Defensive Role of Plant-Derived Secondary Metabolites: Indole and Its’ Derivatives

2020 ◽  
Vol 9 (2) ◽  
pp. 78-88
Author(s):  
Mulugeta Mulat ◽  
Raksha Anand ◽  
Fazlurrahman Khan
Keyword(s):  
Biofilm Formation ◽  
Growth And Development ◽  
Functional Role ◽  
Plant Pathogens ◽  
Bacterial Species ◽  
Indole Derivatives ◽  
Resistance Level ◽  
Defensive Role ◽  
Insect Attack

The diversity of indole concerning its production and functional role has increased in both prokaryotic and eukaryotic systems. The bacterial species produce indole and use it as a signaling molecule at interspecies, intraspecies, and even at an interkingdom level for controlling the capability of drug resistance, level of virulence, and biofilm formation. Numerous indole derivatives have been found to play an important role in the different systems and are reported to occur in various bacteria, plants, human, and plant pathogens. Indole and its derivatives have been recognized for a defensive role against pests and insects in the plant kingdom. These indole derivatives are produced as a result of the breakdown of glucosinolate products at the time of insect attack or physical damages. Apart from the defensive role of these products, in plants, they also exhibit several other secondary responses that may contribute directly or indirectly to the growth and development. The present review summarized recent signs of progress on the functional properties of indole and its derivatives in different plant systems. The molecular mechanism involved in the defensive role played by indole as well as its’ derivative in the plants has also been explained. Furthermore, the perspectives of indole and its derivatives (natural or synthetic) in understanding the involvement of these compounds in diverse plants have also been discussed.

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