Molecular characterization of multidrug-resistant Enterococcus spp. from poultry and dairy farms: detection of virulence and vancomycin resistance gene markers by PCR

There is growing interest in the antimicrobial discovery of life-threatening multidrug-resistant pathogens. The study was undertaken to isolate, identify, and characterize antibiotic-producing actinomycetaceae, particularly nocadisosaceae, from soil samples of Bingol, Turkey. Soil samples were collected from three different regions of Bingol, Turkey. The physicochemical analysis of the soil samples was immediately measured using standard methods. This was followed by isolation of Nocardiopsaceae, nutritional tests, chemotaxonomic analysis, and molecular characterization. The isolated organisms showed morphological properties consistent with the Nocardiopsaceae soil bacteria. The 16s rDNA gene sequence indicated a similarity between the strains with 99.86% which was Nocardiopsaceae synnemata-formans. The BLAST hits had a significant e-value of 0.005. The results of the present study revealed that soil Nocardiopsaceae of Bingol appears to have immense potential as a source of antibacterial compounds.

Download Full-text

Molecular characterization of a second abortive phage resistance gene present in Lactococcus lactis subsp. lactis ME2.

Journal of Bacteriology ◽

10.1128/jb.174.22.7463-7469.1992 ◽

1992 ◽

Vol 174 (22) ◽

pp. 7463-7469 ◽

Cited By ~ 46

Author(s):

E Durmaz ◽

D L Higgins ◽

T R Klaenhammer

Keyword(s):

Resistance Gene ◽

Molecular Characterization ◽

Lactococcus Lactis ◽

Phage Resistance ◽

Lactococcus Lactis Subsp

Download Full-text

Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01792-13 ◽

2014 ◽

Vol 58 (4) ◽

pp. 1997-2005 ◽

Cited By ~ 18

Author(s):

Li-Li Zhao ◽

Yan Chen ◽

Hai-Can Liu ◽

Qiang Xia ◽

Xiao-Cui Wu ◽

...

Keyword(s):

Molecular Characterization ◽

Multidrug Resistant ◽

Diagnostic Methods ◽

Molecular Diagnostic ◽

Beijing Family ◽

Phenotypic Data ◽

Resistant Tuberculosis ◽

Multidrug Resistant Tuberculosis ◽

Mdr Tb

ABSTRACTTo investigate the molecular characterization of multidrug-resistant tuberculosis (MDR-TB) isolates from China and the association of specific mutations conferring drug resistance with strains of different genotypes, we performed spoligotyping and sequenced nine loci (katG,inhA, theoxyR-ahpCintergenic region,rpoB,tlyA,eis,rrs,gyrA, andgyrB) for 128 MDR-TB isolates. Our results showed that 108 isolates (84.4%) were Beijing family strains, 64 (59.3%) of which were identified as modern Beijing strains. Compared with the phenotypic data, the sensitivity and specificity of DNA sequencing were 89.1% and 100.0%, respectively, for isoniazid (INH) resistance, 93.8% and 100.0% for rifampin (RIF) resistance, 60.0% and 99.4% for capreomycin (CAP) resistance, 84.6% and 99.4% for kanamycin (KAN) resistance, and 90.0% and 100.0% for ofloxacin (OFX) resistance. The most prevalent mutations among the MDR-TB isolates werekatG315,inhA15,rpoB531, -526, and -516,rrs1401,eis-10, andgyrA94, -90, and -91. Furthermore, there was no association between specific resistance-conferring mutations and the strain genotype. These findings will be helpful for the establishment of rapid molecular diagnostic methods to be implemented in China.

Download Full-text