Proteomic analysis to understand the relationship between the sarcoplasmic protein patterns and meat organoleptic characteristics in different horse muscles during aging

Meat Science ◽  
2022 ◽  
Vol 184 ◽  
pp. 108686
Author(s):  
Antonella della Malva ◽  
Aristide Maggiolino ◽  
Pasquale De Palo ◽  
Marzia Albenzio ◽  
Josè Manuel Lorenzo ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Protein Patterns ◽  
Organoleptic Characteristics ◽  
Sarcoplasmic Protein ◽  
The Relationship

scholarly journals Proteins-markers of placental insufficiency

2010 ◽  
Vol 56 (5) ◽  
pp. 616-620 ◽  
Author(s):  
T.N. Pogorelova ◽  
V.O. Gunko ◽  
N.A. Drukker ◽  
V.A. Linde
Keyword(s):  
Amniotic Fluid ◽  
Proteomic Analysis ◽  
Placental Insufficiency ◽  
Nkg2d Ligand ◽  
Protein Patterns

The proteomic analysis of the amniotic fluids of women with physiological pregnancy and pregnancy, complicated with placental insufficiency has been carried out on the II and III trimesters. The following difference in protein patterns have been recognized: i) appearance of several proteins lacking in physiological pregnancy; ii) absence of several proteins detectable during physiological pregnancy - hippocalcin-like protein 1, CDC37-like protein, NKG2D ligand 2 (II trimester), CDC37-like protein, NKG2D ligand 2 (III trimester). The established differences in the amniotic fluid spectrum, obviously, have the pathogenetic meaning in the placental insufficiency development. The revealed proteins of distinction may serve as markers of this obstetrical pathology.

Correlation analysis of the organoleptic characteristics of the new toothpaste with the effect of restoring and protecting

2016 ◽  
Vol 12 (2) ◽  
pp. 11-18 ◽  
Author(s):  
Т. Еловикова ◽  
Т. Elovikova ◽  
Н. Михейкина ◽  
N. Mikheykina ◽  
Е. Ермишина ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Correlation Analysis ◽  
Organoleptic Properties ◽  
Organoleptic Characteristics ◽  
The Relationship ◽  
Hygienic Conditions

<p class="p1"><span class="s1">In the article estimation of organoleptic characteristics of the new toothpaste with a revitalizing and desensitivity effect of Sensodyne® Repair &amp; Protect. Organoleptic properties of uoride-containing toothpaste using a new calcium phosphate technology of NovaMin® highly appreciated by all patients. Use of toothpaste within two weeks con rms decrease in hypersensitivity of teeth at all patients with a dental hypersensitivity. Carried out correlation analysis of the relationship between the organoleptic characteristics of the new toothpaste with regenerating and desensitizing effect of Sensodyne® Repair &amp; Protect. Correlation analysis showed that the organoleptic properties of the new toothpaste with regenerating and desensitizing effect have a signi cant impact on the assessment of the hygienic conditions and comfort in the mouth. </span></p>

Fish Species Identification by Isoelectric Focusing: Sarcoplasmic Protein Polymorphism in Monkfish (Lophius americanus)

1981 ◽  
Vol 64 (1) ◽  
pp. 32-37
Author(s):  
Ronald C Lundstrom
Keyword(s):  
Species Identification ◽  
Isoelectric Focusing ◽  
Fish Species ◽  
Protein Pattern ◽  
Polyacrylamide Gel ◽  
Protein Patterns ◽  
Fish Species Identification ◽  
Sarcoplasmic Protein ◽  
Gel Isoelectric Focusing ◽  
Agarose Ief

Abstract Monkfish (Lophius americanus) sarcoplasmic protein patterns were found to be polymorphic with respect to separations using isoelectric focusing. Reproducible protein pattern variations were not detected using cellulose acetate or polyacrylamide gel disc electrophoresis. Monkfish sarcoplasmic proteins separated on pH 3.5-9.5 Ampholine PAGplates or on pH 2.5-9.0 agarose IEF gels yielded similar patterns showing 3 distinct, reproducible variations. On close examination, the pH 3.5-9.5 Ampholine PAGplate patterns could be further subdivided into 3 additional variations. A high resolution pH 3.5-5.0 agarose IEF gel was able to resolve a total of 10 different monkfish pattern variations in a sample of 24 individuals. A model was proposed suggesting the existence of 16 distinct variations in the monkfish sarcoplasmic protein pattern, based on the various combinations of 4 protein bands. In identifying samples of monkfish meat, it is necessary to compare the unknown pattern with the possible variant patterns to effect a reliable identification. On recommendation by the Associate Referee, the method for fish species identification based on polyacrylamide gel isoelectric focusing, 18.A01-18.A04, has been adopted official final action with no restrictions as to the species that may be identified.

Organoleptic assessment of canned dairy products: features of carrying it out and interpreting the results

2021 ◽  
pp. 726-729
Author(s):  
T.B. Guseva ◽  
S.Yu. Soldatova ◽  
O.M. Karanyan
Keyword(s):  
Dairy Products ◽  
Raw Materials ◽  
Physicochemical Characteristics ◽  
Storage Conditions ◽  
Qualitative Properties ◽  
Condensed Milk ◽  
Organoleptic Characteristics ◽  
The Relationship ◽  
And Storage ◽  
Over Time

The article describes the features of carrying out and interpreting the results of the organoleptic assessment of canned dairy products “Whole condensed milk with sugar”. The analysis of the qualitative properties of the product is presented for all the main parameters. The relationship between the organoleptic characteristics of the product and compliance with the technology for the production of canned dairy products, the characteristics of raw materials, technological modes of heat treatment, and storage conditions are considered in detail. Since the organoleptic and physicochemical characteristics of food products change over time, the authors conclude that, in addition to the input evaluation, periodic control of canned dairy products during storage should be provided.

scholarly journals Proteomic Analysis of the Relationship between Metabolism and Nonhost Resistance in Soybean Exposed to Bipolaris maydis

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141264 ◽  
Author(s):  
Yumei Dong ◽  
Yuan Su ◽  
Ping Yu ◽  
Min Yang ◽  
Shusheng Zhu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Nonhost Resistance ◽  
Bipolaris Maydis ◽  
The Relationship

scholarly journals An Optimized Protein Extraction Method for Gel-Free Proteomic Analysis of Opuntia Ficus-Indica

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 115
Author(s):  
Akiko Hashiguchi ◽  
Hisateru Yamaguchi ◽  
Keisuke Hitachi ◽  
Kazuo Watanabe
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Extraction Method ◽  
Protein Extraction ◽  
High Stress ◽  
Ribulose Bisphosphate Carboxylase ◽  
Protein Patterns ◽  
Opuntia Ficus Indica ◽  
Bisphosphate Carboxylase ◽  
Opuntia Spp

Opuntia spp. is an economically important vegetable crop with high stress-tolerance and health benefits. However, proteomic analysis of the plant has been difficult due to the composition of its succulent cladodes; the abundant polysaccharides interfere with protein extraction. To facilitate proteomic analysis of this plant, we present a rapid and simple protein extraction method for Opuntia ficus-indica (L.) Miller. The optimized method produced highly reproducible protein patterns and was compatible with a gel-free quantitative workflow without the need for additional purification. We successfully analyzed the cladode mesocarp and exocarp tissues, resulting in the identification of 319 proteins. In addition, we used this method to examine the relative changes in the Opuntia proteome in response to salt stress to determine whether physiological changes could be captured. Qualified observations were obtained, revealing that salt stress increased phosphoenolpyruvate carboxylase abundance and decreased ribulose-bisphosphate carboxylase in young O. ficus-indica plants. These findings suggest that Crassulacean acid metabolism is promoted under salinity. This study highlights the efficacy of our optimized protein extraction method for elucidating the metabolic adaptations of Opuntia using gel-free proteomic analysis.

Proteomic Analysis of Hypertrophied Myocardial Protein Patterns in Renovascularly Hypertensive and Spontaneously Hypertensive Rats

2006 ◽  
Vol 5 (11) ◽  
pp. 2901-2908 ◽  
Author(s):  
Si-Gui Zhou ◽  
Shu-Feng Zhou ◽  
He-Qing Huang ◽  
Jian-Wen Chen ◽  
Min Huang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Protein Patterns ◽  
Spontaneously Hypertensive

scholarly journals 2-DE-based proteomic analysis of protein changes associated with etiolated mesocotyl growth in Zea mays

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Liangjie Niu ◽  
Zhaokun Wu ◽  
Hui Liu ◽  
Xiaolin Wu ◽  
Wei Wang
Keyword(s):  
Zea Mays ◽  
Proteomic Analysis ◽  
Stress Proteins ◽  
Storage Proteins ◽  
Biological Processes ◽  
Protein Patterns ◽  
Two Dimensional Gel Electrophoresis ◽  
Oxidation Reduction ◽  
Shock Proteins ◽  
Related Proteins

Abstract Background The mesocotyl connects the coleoptilar node and the basal part of the seminal root of maize (Zea mays) seedling. The mesocotyl pushes the shoot of the seedling out of the soil during seed germination; thus, its growth is highly related to deep-sowing tolerance. Although many studies on the maize mesocotyl have been carried out at physiological and molecular levels, the proteomic changes associated with cellular and physiological activities during mesocotyl growth are still unknown. Results In the present study, the maize hybrid Zhengdan 958 was used to study mesocotyl growth and accompanying protein changes. The dark-grown etiolated mesocotyls exhibited a slow-fast-slow feature, with significant changes in the levels of indole-3-acetic acid (IAA) and cellulose and the activity of peroxidase (POD). In particular, POD activity increased with mesocotyl growth, showing higher activity at the mature (lower) end of the mesocotyl. For the proteomic analysis, soluble proteins were extracted from etiolated mesocotyls dark-grown for 48 h, 84 h, and 132 h, corresponding to the initial, rapid, and slow growth periods, respectively, and subjected to separation by two-dimensional gel electrophoresis (2-DE). As a result, 88 differentially abundant proteins (DAPs) were identified using MALDI-TOF-TOF analysis. At 48 h, most DAPs were stress proteins, heat shock proteins and storage proteins; at 84 h, oxidation/reduction proteins, carbohydrate biogenesis-related proteins and cytoskeleton-related proteins were highly accumulated; at 132 h, the most striking DAPs were those involved in the synthesis and modification of the cell wall and the biogenesis of carbohydrates. Gene ontology (GO) analysis showed that changes in the abundance and proportion of DAPs were consistent with cellular and physiological activities and biological processes during mesocotyl growth. The accumulation of nine DAPs of interest was verified by immunoblotting and RT-qPCR. Conclusions The present study revealed that the protein patterns in 2-D gels differed greatly with mesocotyl growth. At different growth periods, a specific set of DAPs participate in various biological processes and underlie the cellular and physiological activities of the mesocotyl. These results contributed to the understanding of mesocotyl growth and the cultivation of maize lines with deep-sowing tolerance.

Comparison of protein patterns ofListeria monocytogenesgrown in biofilm or in planktonic mode by proteomic analysis

2002 ◽  
Vol 210 (1) ◽  
pp. 25-31 ◽  
Author(s):  
F. Trémoulet ◽  
O. Duché ◽  
A. Namane ◽  
B. Martinie ◽  
J.C. Labadie ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Protein Patterns
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