Total antioxidant status before and after bariatric surgery for type 2 diabetes mellitus

Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in Type 2 diabetic patients (with and without complications) and controls.Materials and Methods: Blood samples were collected from 200 patients with type 2 diabetes mellitus (n=100 with complications and n=100 without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). Total antioxidant status was assessed by Ferric Reducing Ability of Plasma (FRAP) assay.Results: TAS was found to be significantly lower in type 2 diabetic patients (with and without complications) compared to controls (p< 0.001). Similarly, patients with complications of type 2 diabetes mellitus had significantly lower TAS when compared to diabetic patients (p= 0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p< 0.001). Fasting glucose and glycosylated haemoglobin level (HbA1c) was found to be significantly higherin diabetic patients than controls (p< 0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA1c, TAS and DNA damage in patients with Type 2 diabetes mellitus.Conclusion: Alterations in TAS and the extent of DNA damage was observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.Key words: Hyperglycemia, Oxidative stress, DNA damage, Total antioxidant status, Type 2 diabetes mellitus, Vascular complication

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Comparison of Antioxidative Effects of Biguanides and Sulfonylureas Monotherapy on Total Antioxidant Status in Newly-Diagnosed Patients with Type 2 Diabetes Mellitus

Diabetes Case Reports ◽

10.4172/2572-5629.1000104 ◽

2016 ◽

Vol 01 (01) ◽

Cited By ~ 1

Author(s):

Siddique MAH ◽

Begum A ◽

Begum S ◽

Khan MH ◽

Saiedullah M ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Newly Diagnosed ◽

Total Antioxidant ◽

Antioxidative Effects

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Medication Utilization and Annual Health Care Costs in Patients With Type 2 Diabetes Mellitus Before and After Bariatric Surgery

Archives of Surgery ◽

10.1001/archsurg.2010.150 ◽

2010 ◽

Vol 145 (8) ◽

pp. 726 ◽

Cited By ~ 69

Author(s):

Martin A. Makary ◽

Jeanne M. Clark ◽

Andrew D. Shore ◽

Thomas H. Magnuson ◽

Thomas Richards ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Bariatric Surgery ◽

Health Care ◽

Type 2 Diabetes Mellitus ◽

Health Care Costs ◽

Medication Utilization ◽

Before And After ◽

Care Costs

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Diabetes Mellitus Tipo 2, Depressão e Alterações do Comportamento Alimentar em Doentes Submetidos a Cirurgia Bariátrica

Acta Médica Portuguesa ◽

10.20344/amp.6399 ◽

2016 ◽

Vol 29 (3) ◽

pp. 176

Author(s):

Isabel Brandão ◽

Ana Marques Pinho ◽

Filipa Arrojado ◽

Ana Pinto-Bastos ◽

José Maia Da Costa ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Bariatric Surgery ◽

Eating Disorders ◽

Weight Loss ◽

Type 2 Diabetes Mellitus ◽

Postoperative Period ◽

Self Report ◽

Before And After

Introduction: Obesity is associated with a great number of complications, including type 2 diabetes mellitus and psychiatric pathology. Bariatric surgery is the best solution to weight loss and improvement of complications in morbid obese patients. This study aims to analyze the evolution of type 2 diabetes mellitus and psychopathologic variables before and after bariatric surgery and assess the importance of different variables in weight loss. Material and Methods: This is a longitudinal study, which evaluates 75 patients before and after bariatric surgery (47 - LAGB – laparoscopic adjustable gastric band; 19 – RYGB – Roux-en-Y gastric bypass; 9 - sleeve) with a follow-up time between 18 and 46 months. A clinical interview and self report questionnaires were applied - Eating Disorder Examination questionnaire – EDE-Q and Beck Depression Inventory – BDI. Results: Results show an improvement in type 2 diabetes mellitus after surgery (χ2 (1) = 26.132, p < 0.001). There was not a significant improvement among psychiatric pathology when we controlled the analysis for the type of surgery. It was verified that type 2 diabetes mellitus, depression and eating disorders in post-operative period are associated with less weight loss. This model explains 27% of weight variance after surgery (R2 = 0.265) and it is significant F (3.33) = 2.981, p = 0.038. Discussion: Type 2 diabetes mellitus, psychiatric pathology and eating disorders after surgery influenced weight loss. It was not clear in what way this relation was verified, neither the relation that these metabolic and psychological variables may have during the postoperative period. Conclusion: Type 2 diabetes mellitus improved after surgery. Type 2 diabetes mellitus, depression and eating disorders influenced weight loss in the postoperative period. These variables did not influence weight loss in the preoperative period.

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ASSOCIATION OF MTHFR A1298C GENE VARIANT, DNA DAMAGE, AND TOTAL ANTIOXIDANT STATUS WITH THE RISK OF TYPE 2 DIABETES MELLITUS AND ITS COMPLICATIONS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25076 ◽

2018 ◽

Vol 11 (6) ◽

pp. 344

Author(s):

Nithya K ◽

Isabel W ◽

Angeline T ◽

Priscilla A.s. ◽

Asirvatham A.j.

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Dna Damage ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Mthfr A1298c ◽

Increased Risk ◽

Significant Difference ◽

Total Antioxidant

Objectives: We have examined the association of methylenetetrahydrofolate reductase (MTHFR) gene A1298C variant, DNA damage, and total antioxidant status (TAS) in patients with type 2 diabetes mellitus (T2DM) with and without complications and in healthy controls.Methods: A total of 300 subjects including 100 patients with complications, 100 patients without complications, and 100 controls were included. TAS was assessed by ferric reducing ability of plasma assay. DNA damage was analyzed in lymphocytes using the comet assay. Polymerase chain reaction-restriction fragment length polymorphism analysis was performed to study the MTHFR A1298C gene polymorphism among the study subjects.Results: The results revealed that the MTHFR 1298 AC+CC genotypes were associated with increased risk (2 fold) for diabetes and its complications. When the effect of DNA damage was analyzed, significant differences between individuals with mutant and normal genotype among the diabetic patients (with and without complications) was observed (p≤0.001). In contrary, no significant difference was found between TAS and 1298 genotypes (AA vs. AC+CC) in Type 2 diabetes patients (with and without complications), p=0.338. We also found a significant difference between the genotypes of the MTHFR A1298C and DNA damage, TAS in T2DM patients (with & without complications) when compared to controls, p<0.001.Conclusions: Our findings suggest that the MTHFR A1298C gene polymorphism is considered as a risk factor for the development of diabetes and its complications among south Indians. Therefore, increased DNA damage and decreased TAS along with the occurrence of a mutant genotype in an individual with diabetes may be at an increased risk for the development of chronic complications.

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