scholarly journals ASSOCIATION OF MTHFR A1298C GENE VARIANT, DNA DAMAGE, AND TOTAL ANTIOXIDANT STATUS WITH THE RISK OF TYPE 2 DIABETES MELLITUS AND ITS COMPLICATIONS

2018 ◽  
Vol 11 (6) ◽  
pp. 344
Author(s):  
Nithya K ◽  
Isabel W ◽  
Angeline T ◽  
Priscilla A.s. ◽  
Asirvatham A.j.
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Mthfr A1298c ◽  
Increased Risk ◽  
Significant Difference ◽  
Total Antioxidant

Objectives: We have examined the association of methylenetetrahydrofolate reductase (MTHFR) gene A1298C variant, DNA damage, and total antioxidant status (TAS) in patients with type 2 diabetes mellitus (T2DM) with and without complications and in healthy controls.Methods: A total of 300 subjects including 100 patients with complications, 100 patients without complications, and 100 controls were included. TAS was assessed by ferric reducing ability of plasma assay. DNA damage was analyzed in lymphocytes using the comet assay. Polymerase chain reaction-restriction fragment length polymorphism analysis was performed to study the MTHFR A1298C gene polymorphism among the study subjects.Results: The results revealed that the MTHFR 1298 AC+CC genotypes were associated with increased risk (2 fold) for diabetes and its complications. When the effect of DNA damage was analyzed, significant differences between individuals with mutant and normal genotype among the diabetic patients (with and without complications) was observed (p≤0.001). In contrary, no significant difference was found between TAS and 1298 genotypes (AA vs. AC+CC) in Type 2 diabetes patients (with and without complications), p=0.338. We also found a significant difference between the genotypes of the MTHFR A1298C and DNA damage, TAS in T2DM patients (with & without complications) when compared to controls, p<0.001.Conclusions: Our findings suggest that the MTHFR A1298C gene polymorphism is considered as a risk factor for the development of diabetes and its complications among south Indians. Therefore, increased DNA damage and decreased TAS along with the occurrence of a mutant genotype in an individual with diabetes may be at an increased risk for the development of chronic complications.

scholarly journals ASSESSMENT OF DNA STRAND BREAKS AND TOTAL ANTIOXIDANT STATUS IN TYPE 2 DIABETIC PATIENTS WITH AND WITHOUT COMPLICATIONS- A CASE-CONTROL STUDY

2017 ◽  
Vol 10 (4) ◽  
pp. 430
Author(s):  
K Nithya ◽  
Isabel W ◽  
Angeline T ◽  
Priscilla As ◽  
Asirvatham Aj
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Total Antioxidant

 Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in Type 2 diabetic patients (with and without complications) and controls.Materials and Methods: Blood samples were collected from 200 patients with type 2 diabetes mellitus (n=100 with complications and n=100 without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). Total antioxidant status was assessed by Ferric Reducing Ability of Plasma (FRAP) assay.Results: TAS was found to be significantly lower in type 2 diabetic patients (with and without complications) compared to controls (p< 0.001).  Similarly, patients with complications of type 2 diabetes mellitus had significantly lower TAS when compared to diabetic patients (p= 0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p< 0.001). Fasting glucose and glycosylated haemoglobin level (HbA1c) was found to be significantly higherin diabetic patients than controls (p< 0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA1c, TAS and DNA damage in patients with Type 2 diabetes mellitus.Conclusion: Alterations in TAS and the extent of DNA damage was observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.Key words: Hyperglycemia, Oxidative stress, DNA damage, Total antioxidant status, Type 2 diabetes mellitus, Vascular complication

Total antioxidant status before and after bariatric surgery for type 2 diabetes mellitus

Metabolism ◽  
2009 ◽  
Vol 58 (9) ◽  
pp. 1366 ◽  
Author(s):  
Sarah L. Prior ◽  
Stephen C. Bain ◽  
Jeffrey W. Stephens ◽  
Imran Alam ◽  
John N. Baxter
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Bariatric Surgery ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Before And After ◽  
Total Antioxidant

scholarly journals Prevalence of osteoporosis and osteoporotic fractures in postmenopausal women with type 2 diabetes mellitus

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Lamia Oulkadi ◽  
Bouchra Amine ◽  
Imane El binoune ◽  
Samira Rostom ◽  
Rachid Bahiri
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Postmenopausal Women ◽  
Mineral Density ◽  
Control Subjects ◽  
Increased Risk ◽  
Significant Difference ◽  
Prevalence Of Osteoporosis

Type 2 diabetes mellitus (T2DM) and osteoporosis are chronic diseases with increasing prevalence. The aim of this study was to determine the prevalence of osteoporosis and osteoporotic fracture in women with T2DM and to identify predictive factors of fracture occurrence. The prevalence of osteoporosis and fractures in postmenopausal women with T2DM was 23.1% and 16.9%, respectively. 46.2% of T2DM patients had normal bone mineral density (BMD) (P<0.01) and 58.5% of control subjects had osteopenia (P<0.01). Incidence of fracture in T2DM patients with osteopenia was significantly increased versus control subjects when stratified according the BMD (P=0.009). By stratifying T2DM patients according to fractures, factors that were significantly associated with occurrence included T2DM duration (P=0.038), use of insulin (P=0.017), and lower BMD (P=0.048). Our study suggests that there was a higher prevalence of fracture in T2DM patients compared to control subjects and a significant difference in BMD was found between the groups. We also showed that insulin use, low BMD, and long duration of T2DM are factors associated with an increased risk of bone fracture.

scholarly journals Lipid Peroxidation and Antioxidant Status in Male and Female Patients with Type 1 and Type 2 Diabetes Mellitus

Med Phoenix ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 10-14
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Prem Prakash Singh ◽  
Tarannum Khatun
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Activity ◽  
Male And Female ◽  
Total Antioxidant

Background: Free radicals are reactive oxygen species which cause lipid peroxidation precipitating many metabolic diseases including Diabetes Mellitus. However, these free radicals are quenched by substances known as antioxidants like vitamin C, vitamin E and several other compounds. Lipid peroxidation and antioxidant status were investigated in patients with Type 1 and Type 2 Diabetes mellitus- Pokhara, Nepal.Methods: The extent of lipid peroxidation was assessed by thiobarbituric acid reactive substances and the antioxidant parameter estimations were total antioxidant activity, Vitamin C and Vitamin E assessed in Type 1 and 2 diabetes mellitus patients along with matched healthy counterparts.Results: The lipid peroxidation was increased in male Type 1 and 2 diabetic patients whereas female group showed decreased level as compared to its healthy counterparts. Similarly, the total antioxidant activity was found to be decreased in the diabetic group. The lipid peroxidation parameter and antioxidant status were statistically significant at p< 0.05.Conclusion: Oxidative stress and antioxidant status varied in male and female patients suffering from diabetes either Type 1 or Type 2. Apart from gender basis of evaluating oxidative stress, variables based on diet, habitat, socioeconomic status, education, etc. can also be considered.MED Phoenix Volume (1), Issue (1) July 2016, page: 10-14

scholarly journals Total antioxidant status in type 2 diabetes mellitus with diabetic nephropathy

2019 ◽  
Vol 6 (3) ◽  
pp. 673
Author(s):  
Heena Singla ◽  
Gitanjali Goyal ◽  
Cheenu Garg ◽  
Kajal Bhalla
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Total Antioxidant ◽  
Type 2 Diabetic

Background: Diabetes mellitus has emerged as one of the most common health hazard all over the world. Diabetic nephropathy is the most challenging long term complication of Type 2 Diabetes mellitus and microalbuminuria is the earliest marker of diabetic nephropathy. In diabetes, chronic hyperglycemia and deranged lipid profile lead to excess generation of free radicals. The increased oxidative stress plays a major role in pathogenesis of diabetic complications, including diabetic nephropathy. There are many naturally occurring antioxidant enzymes in our body. Diabetes has multiple effects on protein levels and activity of these antioxidant enzymes. This further augments the oxidative stress. There are many non-enzymatic antioxidants in our body which include vitamins A, C, E and trace minerals like copper, zinc, manganese and selenium.Methods: The study was done on a total of 150 subjects. Group A comprised of 60 Type 2 diabetic patients with diabetic nephropathy, Group B comprised of 60 Type 2 diabetic patients without diabetic nephropathy and Group C comprised 30 healthy controls. Total antioxidant status, microalbuminuria and glycosylated haemoglobin were measured.Results: In present study, authors found that total antioxidant status is drastically reduced in all diabetic patients, and it was found to be further low in patients with diabetic nephropathy. This decrease was found to be directly proportional to the degree of diabetic nephropathy, as measured by the levels of microalbuminuria.Conclusions: Timely institution of antioxidant supplementation therapy may emerge as a promising measure in delaying the onset and progression of diabetic complications, especially diabetic nephropathy.

Effect of a high monounsaturated fatty acid diet alone or with combined vitamin E and C, or lycopene intake on oxidative stress in patients with type 2 diabetes mellitus

2012 ◽  
Vol 12 (2) ◽  
pp. 81-86
Author(s):  
Maha Badkook ◽  
Fiona McCullough ◽  
Nessar Ahmed
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Fatty Acid ◽  
Vitamin E ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Total Antioxidant ◽  
Mufa Diet ◽  
Vitamin E And C

The aims of this study were to investigate the effect of a high monounsaturated fatty acid (MUFA) diet alone or with combined vitamin E and C, or lycopene intake on oxidative stress in type 2 diabetes in Saudi Arabia. Forty-eight type 2 diabetic patients consumed a high MUFA diet for 16 weeks. After four weeks of high MUFA diet alone, supplements of vitamins E (400 mg) and C (1,000 mg) were taken for four weeks, followed by a four-week washout period. In the final four weeks, subjects consumed a high MUFA diet with tomato paste (equivalent to 12 mg lycopene). Plasma samples were tested for vitamin E and C, lycopene, malondialdehyde (MDA), total antioxidant status, fasting plasma glucose and glycated haemoglobin A1c (HbA1c). A high-MUFA diet with vitamins E and C or lycopene caused significant elevation of plasma vitamins E, C and lycopene compared to a high-MUFA diet alone. Plasma MDA was reduced with vitamins, but not lycopene supplementation. The total antioxidant status increased significantly following a high-MUFA diet and with vitamin and lycopene supplementations. Fasting glucose was not affected whereas HbA1c decreased significantly after vitamin supplementation compared to baseline. A high-MUFA diet supplemented with vitamin E and C, or lycopene improves antioxidant status in type 2 diabetes.

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