Antibacterial activity of Artemisia asiatica essential oil against some common respiratory infection causing bacterial strains and its mechanism of action in Haemophilus influenzae

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

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Antibacterial Effects of Oregano Essential Oil (OEO) and Its Potential Applications

Microbiology Research Journal International ◽

10.9734/mrji/2020/v30i430209 ◽

2020 ◽

pp. 19-25

Author(s):

R. Cabrera-Contreras ◽

R. Morelos-Ramírez ◽

J. P. Quiróz-Ríos ◽

D. Muñoz-Quiróz

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Staphylococcus Epidermidis ◽

Drug Resistant ◽

Human Pathogens ◽

Bacterial Strains ◽

Methicillin Resistant ◽

Reference Strains ◽

Serial Dilutions

Essential oils (EOs) are commonly used in food industry, due that they possess antioxidative and antimicrobial properties. There are few essential oils that have been used in medicine, due to its potent antibacterial activity against intrahospital pathogens. OEO has experimentally shown potent antibacterial effect on nosocomial Gram-positive bacteria, therefore it can be very useful in hospital environments, where there are many bacterial pathogens, which are the etiological agents of nosocomial infections and most of them are resistant to several antibiotics. Objective: The aim of this study was to determine antimicrobial effect of OEO on most frequent bacterial intrahospital pathogens: MRSA, MRSE comparatively to selected ATCC bacterial reference strains. Methods: This experimental study investigates the antibacterial action of oregano (Origanum vulgare) essential oil (OvEO) on two human pathogens: Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE) Here, we used OEO against one of the most prominent antibiotic-resistant bacterial strains: methicillin-resistant SA (MRSAmecA+ = Meticillin Resistant SA and mecA- = Meticillin Resistance SA ), methicillin-resistant SE (MRSEmecA+ = Meticillin Resistance Staphylococcus epidermidis mecA+) and reference strains: S. aureus ATCC 700699, S. epidermidis ATCC 359845 and E. coli ATCC 25922. Bactericidal effects of the OEO on these bacteria were mainly evaluated using undiluted and four serial dilutions in coconut oil (CCO) l: 1:10, 1:100, 1:200, 1:400. Results: OEO, undiluted and 4 serial dilutions showed potent antibacterial activity against all strains tested. In conclusion, this OEO could be used as an alternative in medicine. The ability of OEO to inhibit and kill clinical Multi-Drug-Resistant (MDR): MRSA and MRSE strains, highlights it´s potential for use in the management of drug-resistant MDR infections in hospitals wards.

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Essential oil from pink pepper (Schinus terebinthifolius Raddi): Chemical composition, antibacterial activity and mechanism of action

Food Control ◽

10.1016/j.foodcont.2018.07.034 ◽

2019 ◽

Vol 95 ◽

pp. 115-120 ◽

Cited By ~ 12

Author(s):

Guilherme da Silva Dannenberg ◽

Graciele Daiana Funck ◽

Wladimir Padilha da Silva ◽

Ângela Maria Fiorentini

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Mechanism Of Action ◽

Schinus Terebinthifolius

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Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x17751314 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1775131 ◽

Cited By ~ 7

Author(s):

Farhad Sharafati Chaleshtori ◽

Mohamad Saholi ◽

Reza Sharafati Chaleshtori

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Antioxidant Power ◽

Listeria Species ◽

Bunium Persicum

This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp. L monocytogenes was found to be resistant to ampicillin, amoxicillin/clavulanic acid, penicillin, vancomycin, and kanamycin. B persicum essential oil showed the greatest antioxidant activity (248.56 ± 1.09 µM Fe2+/g). The E globulus essential oil showed consistently strong antimicrobial activity against L monocytogenes and L grayi, while rose water showed no antimicrobial activity against any of the tested bacterial strains. The results showed that after adding the B persicum and E globulus essential oils to bacteria, the cell components’ release increased significantly.

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Chemical Composition and Antibacterial Activity of Essential oil from Salvia mukerjeei

Natural Product Communications ◽

10.1177/1934578x1100601239 ◽

2011 ◽

Vol 6 (12) ◽

pp. 1934578X1100601 ◽

Cited By ~ 1

Author(s):

Lalit Mohan ◽

Anuradha Negi ◽

Anand B. Melkani ◽

Vasu Dev

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Agrobacterium Tumefaciens ◽

Essential Oil ◽

Volatile Oil ◽

Erwinia Chrysanthemi ◽

Bacterial Strains ◽

Capillary Gc ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons

The composition of steam volatile oil from aerial parts of Salvia mukerjeei Bennet & Raizada (Lamiaceae) was analyzed by capillary GC and GCMS. The oil was rich in sesquiterpene hydrocarbons (67.3%). Among 71 identified constituents representing 91.7% of the oil, β-caryophyllene (28.7%), γ-muurolene (15.5%) and dehydro-aromadendrane (9.5%), were the principal constituents. The oil was tested against ten bacterial strains and was active against Enterococcus faecalis, Erwinia chrysanthemi and Agrobacterium tumefaciens.

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Antibacterial Activity of the Essential Oil of Heracleum Sibiricum

Natural Product Communications ◽

10.1177/1934578x1300800931 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 4

Author(s):

Dragoljub L. Miladinović ◽

Budimir S. Ilić ◽

Tatjana M. Mihajilov-Krstev ◽

Dejan M. Nikolić ◽

Olga G. Cvetković ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Antimicrobial Activities ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Major Chemical ◽

Total Oil ◽

Chemical Class

The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 μg/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 μg/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.

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Antibacterial Activity and Essential Oil Composition of Satureja spicigera from Iran

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-1-204 ◽

2009 ◽

Vol 64 (1-2) ◽

pp. 20-24 ◽

Cited By ~ 21

Author(s):

Fereshteh Eftekhar ◽

Fereshteh Raei ◽

Morteza Yousefzadi ◽

Samad Nejad Ebrahimi ◽

Javad Hadian

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oil Composition ◽

Diffusion Method ◽

Resistant Organism ◽

Disc Diffusion ◽

Oil Composition ◽

Bacterial Strains ◽

Full Flowering

The aerial parts of Satureja spicigera were collected at full flowering stage at Gazvin, Iran. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Fourteen compounds were identified, of which carvacrol (53.74%) and thymol (36.03%) were the main constituents, representing 99.12% of the total oil. The in vitro antibacterial activity of the essential oil was determined against six ATCC standard bacterial strains (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) using disc diffusion as well as measurement of minimum inhibitory concentrations. The disc diffusion results and MIC values indicated high inhibitory activity against the test bacteria. The most susceptible organisms were the Gram-positive B. subtilis and S. aureus followed by E. faecalis, usually resistant to most common antibiotics. Among the Gram-negative bacteria, E. coli and K. pneumoniae were highly sensitive to the different oil concentrations in the disc diffusion method. Finally, P. aeruginosa, a highly resistant organism to most antibiotics, showed moderate susceptibility to Satureja spicigera essential oil.

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