scholarly journals Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ζ

2020 ◽  
Vol 79 (6) ◽  
pp. 1037-1050.e5
Author(s):  
Chance Meers ◽  
Havva Keskin ◽  
Gabor Banyai ◽  
Olga Mazina ◽  
Taehwan Yang ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Genetic Characterization

Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant

1984 ◽  
Vol 26 (3) ◽  
pp. 386-389 ◽  
Author(s):  
Linda J. Reha-Krantz ◽  
Sükran Parmaksizoglu
Keyword(s):  
Dna Polymerase ◽  
Low Temperatures ◽  
Bacteriophage T4 ◽  
Effect Of Temperature ◽  
In Vitro Mutagenesis ◽  
Wild Type ◽  
Mutational Site

The effect of temperature on genetically well-defined mutational pathways was examined in the bacteriophage T4. The mutational site was a T4 rII ochre mutant which could revert to rII+ via a transversion or to the amber convertant via a transition. Temperature did not strongly affect any of the pathways examined in a wild-type background; however, increased temperature reduced the mutational activity of a mutator DNA polymerase mutant. Possible models to explain the role of temperature in mutagenesis are discussed as well as the significance of low temperatures for in vitro mutagenesis reactions.Key words: bacteriophage T4, mutator, transition, transversion, temperature effects.

Biological and genetic characterization of the role of SRSF2 mutations in the pathogenesis of myelodysplastic syndromes

2015 ◽  
Vol 43 (9) ◽  
pp. S73
Author(s):  
Ayana Kon ◽  
Satoshi Yamazaki ◽  
Keisuke Kataoka ◽  
Tetsuichi Yoshizato ◽  
Yusuke Shiozawa ◽  
...  
Keyword(s):  
Myelodysplastic Syndromes ◽  
Genetic Characterization

scholarly journals Role of the INDETERMINATE DOMAIN Genes in Plants

2019 ◽  
Vol 20 (9) ◽  
pp. 2286 ◽  
Author(s):  
Manu Kumar ◽  
Dung Thi Le ◽  
Seongbin Hwang ◽  
Pil Joon Seo ◽  
Hyun Uk Kim
Keyword(s):  
Plant Architecture ◽  
Genetic Characterization ◽  
Sugar Metabolism ◽  
Hormone Signaling ◽  
Biological Functions ◽  
Transcription Factor Family ◽  
Physiological Processes ◽  
Ammonium Metabolism

The INDETERMINATE DOMAIN (IDD) genes comprise a conserved transcription factor family that regulates a variety of developmental and physiological processes in plants. Many recent studies have focused on the genetic characterization of IDD family members and revealed various biological functions, including modulation of sugar metabolism and floral transition, cold stress response, seed development, plant architecture, regulation of hormone signaling, and ammonium metabolism. In this review, we summarize the functions and working mechanisms of the IDD gene family in the regulatory network of metabolism and developmental processes.

scholarly journals Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa

2004 ◽  
Vol 186 (9) ◽  
pp. 2523-2531 ◽  
Author(s):  
M. Schirm ◽  
S. K. Arora ◽  
A. Verma ◽  
E. Vinogradov ◽  
P. Thibault ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gene Cluster ◽  
Genetic Characterization ◽  
Type A ◽  
Protein Backbone ◽  
Central Surface

ABSTRACT Type a flagellins from two strains of Pseudomonas aeruginosa, strains PAK and JJ692, were found to be glycosylated with unique glycan structures. In both cases, two sites of O-linked glycosylation were identified on each monomer, and these sites were localized to the central, surface-exposed domain of the monomer in the assembled filament. The PAK flagellin was modified with a heterogeneous glycan comprising up to 11 monosaccharide units that were O linked through a rhamnose residue to the protein backbone. The flagellin of JJ692 was less complex and had a single rhamnose substitution at each site. The role of the glycosylation island gene cluster in the production of each of these glycosyl moieties was investigated. These studies revealed that the orfA and orfN genes were required for attachment of the heterologous glycan and the proximal rhamnose residue, respectively.

scholarly journals Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir

2005 ◽  
Vol 86 (11) ◽  
pp. 3153-3162 ◽  
Author(s):  
F. Bernardi ◽  
S. A. Nadin-Davis ◽  
A. I. Wandeler ◽  
J. Armstrong ◽  
A. A. B. Gomes ◽  
...  
Keyword(s):  
Animal Species ◽  
Genetic Characterization ◽  
Variable Region ◽  
Epidemiological Studies ◽  
Genetic Classification ◽  
Livestock Species ◽  
Central Variable Region ◽  
Vampire Bats

Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.

Dynamic Characterization of a DNA Repair Enzyme:  NMR Studies of [methyl-13C]Methionine-Labeled DNA Polymerase β

Biochemistry ◽  
2004 ◽  
Vol 43 (28) ◽  
pp. 8911-8922 ◽  
Author(s):  
Bidisha Bose-Basu ◽  
Eugene F. DeRose ◽  
Thomas W. Kirby ◽  
Geoffrey A. Mueller ◽  
William A. Beard ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Dynamic Characterization ◽  
Dna Polymerase Β ◽  
Nmr Studies ◽  
Repair Enzyme

scholarly journals Characterization of an African Swine Fever Virus 20-kDa DNA Polymerase Involved in DNA Repair

1997 ◽  
Vol 272 (49) ◽  
pp. 30899-30910 ◽  
Author(s):  
Mariano Oliveros ◽  
Rafael J. Yáñez ◽  
Marı́a L. Salas ◽  
José Salas ◽  
Eladio Viñuela ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Biochemical characterization of human DNA polymerase i provides clues to its biological function

2001 ◽  
Vol 29 (2) ◽  
pp. 183-187 ◽  
Author(s):  
A. Tissier ◽  
E. G. Frank ◽  
J. P. McDonald ◽  
A. Vaisman ◽  
A. R. Fernàndez deHenestrosa Henestrosa ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Biochemical Characterization ◽  
Short Review ◽  
Biochemical Properties ◽  
Dna Polymerase I ◽  
Polymerase I

The human RAD30B gene has recently been shown to encode a novel DNA polymerase, DNA polymerase i (poli). The role of poli within the cell is presently unknown, and the only clues to its cellular function come from its biochemical characterization in vitro. The aim of this short review is, therefore, to summarize the known enzymic activities of poli and to speculate as to how these biochemical properties might relate to its in vivo function.

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