Statistics assisted analysis of Raman spectra and imaging of human colon cell lines – Label free, spectroscopic diagnostics of colorectal cancer

A novel series of 4-anilinoquinazoline analogues, DW (1–10), were evaluated for anticancer efficacy in human breast cancer (BT-20) and human colorectal cancer (CRC) cell lines (HCT116, HT29, and SW620). The compound, DW-8, had the highest anticancer efficacy and selectivity in the colorectal cancer cell lines, HCT116, HT29, and SW620, with IC50 values of 8.50 ± 2.53 µM, 5.80 ± 0.92 µM, and 6.15 ± 0.37 µM, respectively, compared to the non-cancerous colon cell line, CRL1459, with an IC50 of 14.05 ± 0.37 µM. The selectivity index of DW-8 was >2-fold in colon cancer cells incubated with vehicle. We further determined the mechanisms of cell death induced by DW-8 in SW620 CRC cancer cells. DW-8 (10 and 30 µM) induced apoptosis by (1) producing cell cycle arrest at the G2 phase; (2) activating the intrinsic apoptotic pathway, as indicated by the activation of caspase-9 and the executioner caspases-3 and 7; (3) nuclear fragmentation and (4) increasing the levels of reactive oxygen species (ROS). Overall, our results suggest that DW-8 may represent a suitable lead for developing novel compounds to treat CRC.

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Modulation of the urokinase receptor in human colon cell lines by N,N-dimethylformamide

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/0167-4889(88)90227-3 ◽

1988 ◽

Vol 970 (1) ◽

pp. 96-100 ◽

Cited By ~ 5

Author(s):

Douglas Boyd ◽

Germaine Florent ◽

Genesio Murano ◽

Michael Brattain

Keyword(s):

Cell Lines ◽

Human Colon ◽

Urokinase Receptor ◽

Colon Cell

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Evaluation of the potential anti-cancer activity of the antidepressant sertraline in human colon cancer cell lines and in colorectal cancer-xenografted mice

International Journal of Oncology ◽

10.3892/ijo_00000007 ◽

1992 ◽

Cited By ~ 5

Author(s):

Irit Gil-Ad

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Cell Lines ◽

Human Colon ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Human Colon Cancer ◽

Colon Cancer Cell Lines ◽

Anti Cancer ◽

Cancer Activity

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Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines

Molecular Nutrition & Food Research ◽

10.1002/mnfr.200500194 ◽

2006 ◽

Vol 50 (4-5) ◽

pp. 413-417 ◽

Cited By ~ 31

Author(s):

Sandra Schaefer ◽

Matthias Baum ◽

Gerhard Eisenbrand ◽

Christine Janzowski

Keyword(s):

Cell Lines ◽

Apple Juice ◽

Cell Damage ◽

Human Colon ◽

Colon Cell

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Effect of neuropilin-2 expression on TGF-β1-epithelial-mesenchymal transition in colorectal cancer cells.

Journal of Clinical Oncology ◽

10.1200/jco.2011.29.4_suppl.414 ◽

2011 ◽

Vol 29 (4_suppl) ◽

pp. 414-414

Author(s):

C. Grandclement ◽

R. Bedel ◽

B. Kantelip ◽

E. Viel ◽

J. Remy Martin ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Epithelial Mesenchymal Transition ◽

Human Colon ◽

Mesenchymal Transition ◽

Colorectal Cancer Cells ◽

Tgf Β1

414 Background: Initially characterized as neuronal receptors, Neuropilins (NRPs) were also found to be expressed in endothelial cells and subsequently were shown to play a role in the development of the vascular system. NRP family consists of two genes, neuropilin-1 (NRP1) and neuropilin-2 (NRP2).The multiple functions of NRPs were recently highlighted by the identification of NRP role in oncogenesis. In this study, we first confirmed the role of NRP2 in tumor progression. We also extended the understanding of NRP2 oncogenic functions by investigating the ability of NRP2 to orchestrate epithelial-mesenchymal transition (EMT) in colorectal cancer cells. Methods: We have generated human colon cancer cell lines transfected with NRP2 transgene or siRNA to investigate NRP2 involvement in EMT. First, the oncogenic functions of NRP2 were studied in vitro by MTT, soft agar, invasion assays and in vivo using xenografts experiments. Ability of NRP2 to orchestrate EMT was then investigated by flow cytometry, immunohistochemical (IHC) staining, western-blotting and quantitative real-time PCR. Results: IHC staining revealed that NRP2 is expressed in human colon and breast carcinomas while it is not expressed in healthy tissues. Then, we confirmed that NRP2 increases tumor proliferation, colony formation, invasion and xenograft formation. Moreover, NRP2-expressing cells displayed an immunohistochemical phenotype of EMT characterized by the loss of E-Cadherin and an increase of vimentin. Furthermore, NRP2 expression promotes transforming-growth factor-β1 (TGF- β1) signaling, leading to an increased phosphorylation of the Smad2/3 complex in colorectal cancer cell lines. Specific inhibition of NRP2 using siRNA or treatment with specific TGFβRI kinase inhibitors prevented this phosphorylation and the EMT, suggesting that NRP2 cooperates with TGFRI to promote EMT in colorectal carcinoma. Conclusions: Our findings have reinforced the essential role of NRP2 in cancer progression and demonstrated that NRP2 expression confers to tumor cell lines the hallmarks of EMT. Moreover, in the current work, we present evidence for the therapeutic value of NRP2 targeting. No significant financial relationships to disclose.

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Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines

PROTEOMICS ◽

10.1002/pmic.201700242 ◽

2018 ◽

Vol 18 (8) ◽

pp. 1700242 ◽

Cited By ~ 3

Author(s):

Hwee Tong Tan ◽

Maxey Ching Ming Chung

Keyword(s):

Colorectal Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Colorectal Cancer Cell ◽

Label Free ◽

Colorectal Cancer Cell Lines ◽

Stathmin 1 ◽

Quantitative Phosphoproteomics

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UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition

Journal of Clinical Medicine ◽

10.3390/jcm8040560 ◽

2019 ◽

Vol 8 (4) ◽

pp. 560 ◽

Cited By ~ 5

Author(s):

Javier Martinez-Useros ◽

Nuria Garcia-Carbonero ◽

Weiyao Li ◽

Maria J. Fernandez-Aceñero ◽

Ion Cristobal ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Lines ◽

Cancer Progression ◽

Epithelial To Mesenchymal Transition ◽

Rna Binding ◽

Human Colon ◽

Human Colon Cancer ◽

Mesenchymal Transition ◽

Colorectal Cancer Progression

CSDE1 (cold shock domain containing E1) gene is located upstream of the N-RAS locus, and codes for an RNA-binding protein named Upstream of N-Ras (UNR). In cancer, CSDE1 has been shown to regulate c-Fos, c-Myc, Pten, Rac1, or Vimentin. UNR/CSDE1 has been studied in breast, melanoma, pancreatic and prostate cancer. Then, the aim of this study is to evaluate the role of CSDE1/UNR in colorectal cancer progression and maintenance of aggressive phenotype. We firstly evaluated UNR/CSDE1 expression in human colon cancer derived cell lines and patient samples. Subsequently, we performed functional experiments by UNR/CSDE1 downregulation. We also evaluated UNR/CSDE1 prognostic relevance in two independent sets of patients. Not only was UNR/CSDE1 expression higher in tumor samples compared to untransformed samples, but also in colonospheres and metastatic origin cell lines than their parental and primary cell lines, respectively. Downregulation of UNR/CSDE1 reduced cell viability and migration throughout a restrain of epithelial-to-mesenchymal transition and increases sensitivity to apoptosis. Interestingly, high UNR/CSDE1 expression was associated with poor prognosis and correlated positively with c-MYC expression in colorectal cancer samples and cell lines. Here, we show for the first time compelling data reporting the oncogenic role of UNR/CSDE1 in human colorectal cancer.

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