Evaluation of DNA binding, DNA cleavage, protein binding, radical scavenging and in vitro cytotoxic activities of ruthenium(II) complexes containing 2,4-dihydroxy benzylidene ligands

Four new binuclear nickel(ii) metallates showed promising antiproliferative activity against MCF-7 and HeLa cell lines and were much less toxic against HaCaT.

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Synthesis, characterization, DNA binding, DNA cleavage, protein binding and cytotoxic activities of Ru(II) complexes

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2015.09.045 ◽

2016 ◽

Vol 82 ◽

pp. 663-670 ◽

Cited By ~ 18

Author(s):

Sreekanth Thota ◽

Srujana Vallala ◽

Rajeshwar Yerra ◽

Daniel Alencar Rodrigues ◽

Nulgumnalli Manjunathaiah Raghavendra ◽

...

Keyword(s):

Dna Binding ◽

Protein Binding ◽

Dna Cleavage ◽

Cytotoxic Activities

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Synthesis and Characterization of Oxidovanadium(IV) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino)methyl)-6-methoxyphenol and Their Antimicrobial, Antioxidant, and DNA-Binding Studies

Bioinorganic Chemistry and Applications ◽

10.1155/2018/2452869 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 9

Author(s):

K. Savithri ◽

H. D. Revanasiddappa

Keyword(s):

Dna Binding ◽

Mixed Ligand Complex ◽

Radical Scavenging ◽

Molar Conductance ◽

Diffusion Method ◽

Spectral Studies ◽

Binding Studies ◽

Ligand Complex ◽

Intercalative Mode

Two novel oxidovanadium(IV) complexes with a new bidentate (O- and N-) imine-based ligand 2-((E)-(6-fluorobenzo[d]thiazol-2-ylimino)methyl)-6-methoxyphenol (HL) were synthesized under in situ experimental condition where VOSO4 acts as a kinetic template in the ratio 2 : 1 (L : M) and mixed ligand complex using 1,10-phenanthroline (phen) in 1 : 1 : 1 (L : M : phen) ratio. The synthesized compounds were structurally characterized by microanalysis, magnetic susceptibility, FTIR, electronic spectra, TG/DTA, ESR, and molar conductance studies. Based on the spectral studies, the complexes have the general composition [VO(L)2] (C1) and [VO(L)phen] (C2) in a square pyramid geometrical fashion. The synthesized compounds were primarily screened for their in vitro growth inhibiting activity against different strains of bacteria, namely, E. coli, B. subtilis, S. aureus, and P. aeruginosa by the disc diffusion method. Also, the antifungal activity was determined against C. albicans and A. niger by the Bateman poisoned technique. The in vitro antioxidant activity of all the compounds was determined by DPPH free radical-scavenging assay. Intercalative mode of DNA-binding properties of the oxidovanadium(IV) complexes with calf-thymus DNA (CT-DNA) was investigated using UV, fluorescence spectra, and viscosity measurements.

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An investigation on the DNA/protein binding, DNA cleavage and in vitro anticancer properties of SNO pincer type palladium(II) complexes with N-substituted isatin thiosemicarbazone ligands

Inorganica Chimica Acta ◽

10.1016/j.ica.2017.05.044 ◽

2017 ◽

Vol 466 ◽

pp. 61-70 ◽

Cited By ~ 22

Author(s):

Mathiyan Muralisankar ◽

Sabeel M. Basheer ◽

Jebiti Haribabu ◽

Nattamai S.P. Bhuvanesh ◽

Ramasamy Karvembu ◽

...

Keyword(s):

Protein Binding ◽

Dna Cleavage ◽

Anticancer Properties

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Transcriptional control of the factor IX gene: analysis of five cis- acting elements and the deleterious effects of naturally occurring hemophilia B Leyden mutations

Blood ◽

10.1182/blood.v84.9.2992.2992 ◽

1994 ◽

Vol 84 (9) ◽

pp. 2992-3000 ◽

Cited By ~ 22

Author(s):

DJ Picketts ◽

CR Mueller ◽

D Lillicrap

Keyword(s):

Dna Binding ◽

Protein Binding ◽

Transcriptional Activation ◽

In Vitro Transcription ◽

Factor Ix ◽

Hemophilia B ◽

Transcription Assay ◽

Cis Acting ◽

Nuclear Extracts

Abstract Hemophilia B Leyden is a rare form of inherited factor IX deficiency in which patients experience spontaneous postpubertal recovery of factor IX levels. The mutations resulting in this disorder are localized in a 40-nucleotide region encompassing the major transcriptional start site for factor IX. Here we report the further characterization of five cis- acting elements in the factor IX promoter and the effects on protein binding and transcriptional activation of five Leyden mutations (at nucleotides +13, -5, -6, -20, and -26) that occur within the proximal three elements (sites 1 through 3). Bandshift studies using nuclear extracts from four different rat tissues have shown that at least some of the proteins binding to each of the five sites are ubiquitous in nature. The pattern of DNA binding at site 1 suggests that this element plays an important role in mediating the liver-specific expression of factor IX. Additional studies with liver nuclear extracts obtained at several different points in development have shown an increase in DNA binding at sites 1, 4, and 5 between 1 day and 1 week. Using DNase I footprint analysis and competition bandshift studies, we have shown that the binding of nuclear proteins to each of the mutant sites is disrupted to a variable extent. There appears to be some, although reduced, protein binding to all of the mutant oligonucleotides apart from the -26 mutant. In vitro transcription assays have shown that each of the mutations reduces the global proximal promoter activity by approximately 40%. Two double mutant promoters did not show any additional downregulation in the in vitro transcription assay. In experiments designed to assess the relative transcriptional activity mediated from each of the five sites independently, we have tested artificial homopolymer promoters of each site in the in vitro transcription assay. These studies show that sites 4 and 5 are the strongest activators and that transactivation from site 5 is further enhanced by the albumin D site-binding protein. In summary, these investigations show deleterious effects of each of the Leyden mutations tested on the binding of trans-acting factors and also show disruption of transcriptional activation in a functional in vitro transcription assay. Our results also show that cis-acting elements 4 and 5 are the principal activators of this locus.

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Behaviour of 9-Ethyl-9H-carbazole Hydrazone Derivatives Against Oxidant Systems

Croatica Chemica Acta ◽

10.5562/cca3481 ◽

2019 ◽

Vol 92 (1) ◽

pp. 87-94 ◽

Cited By ~ 2

Author(s):

Cigdem Karaaslan ◽

Hande Gurer-Orhan ◽

Sibel Suzen ◽

Luciano Saso ◽

Omidreza Firuzi ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Amyloid Β ◽

In Vitro Assays ◽

Cytotoxic Activities ◽

Scavenging Activity ◽

Neuroprotective Effects ◽

Neuronal Pc12 Cells

Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.

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In vivo Neurological, Analgesic and In vitro Antioxidant and Cytotoxic Activities of Ethanolic Extract of Leaf and Stem Bark of Wedelia chinensis

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v22i1.40021 ◽

2019 ◽

Vol 22 (1) ◽

pp. 18-26

Author(s):

Sayema Khanum ◽

Md Shahid Sarwar ◽

Mohammad Safiqul Islam

Keyword(s):

Body Weight ◽

Oral Administration ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Pharmaceutical Journal ◽

Stem Bark ◽

Cytotoxic Activities

Wedelia chinensis is a widely used anti-inflammatory and hepatoprotective medicinal plant in Bangladesh. In this study, analgesic, neurological, antioxidant and cytotoxic activities of the ethanolic extract of leaf and stem bark of W. chinensis were investigated. Oral administration of the ethanolic extract of W. chinensis (200- and 300-mg/kg body weight) was investigated on animal model for neurological activity using open field test and hole cross test. Acetic acid induced writhing method was used to assess the analgesic activity. DPPH (1,1-diphenyl, 2-picryl hydrazyl) radical scavenging assay was used for determining the antioxidant activity, while brine shrimp lethality bioassay was used for investigating cytotoxicity. The ethanol extract of the plant produced significant reduction (P<0.05) of locomotion in both doses (200- and 300-mg/kg body weight) indicating pronounced neurological activity. Oral administration of alcoholic leaves and stem extracts significantly (p < 0.05) inhibited writhing response in mice. The percentage of scavenging of DPPH free radical was found to be concentration dependent with IC50 value of 44.10 ± 0.65 and 38.96 ± 0.50 μg/ml for leaves and stem extracts, respectively. Our findings indicate that W. chinensis may be a source of natural antioxidant with potent analgesic, neurological and cytotoxic activities. Bangladesh Pharmaceutical Journal 22(1): 18-26, 2019

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Synthesis, Crystal Structure, DNA-binding Properties and Antioxidant Activities of a Lutetium(III) Complex with the Bis(N-salicylidene)-3- oxapentane-1,5-diamine Ligand

Zeitschrift für Naturforschung B ◽

10.5560/znb.2013-2339 ◽

2013 ◽

Vol 68 (3) ◽

pp. 257-266 ◽

Cited By ~ 18

Author(s):

Guolong Pan ◽

Yuchen Bai ◽

Hua Wang ◽

Jin Kong ◽

Furong Shi ◽

...

Keyword(s):

Crystal Structure ◽

Dna Binding ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Binding Mode ◽

Binding Properties ◽

Metal Centers ◽

Spectrophotometric Methods ◽

Dna Binding Properties

A Schiff base ligand bis(N-salicylidene)-3-oxapentane-1,5-diamine (H2L) and its lutetium(III) complex, with composition Lu2(L)2(NO3)2, were synthesized and characterized by physico-chemical and spectroscopic methods. The crystal structure of the Lu(III) complex has been determined by single-crystal X-ray diffraction. It reveals a centrosymmetric binuclear neutral entity where Lu(III) metal centers are bridged by two phenoxo oxygen atoms. The DNA-binding properties of the Lu(III) complex were investigated by spectrophotometric methods and viscosity measurements, and the results suggest that the Lu(III) complex binds to DNA via a groove binding mode. Additionally, the antioxidant activity of the Lu(III) complex was determined by the superoxide and hydroxyl radical scavenging methods in vitro, which indicate that it is a scavenger for OH· and O-· 2 radicals.

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