MKL, an actin-binding coactivator for SRF, is involved in activin-induced alteration of dendritic morphology and transcriptional activity in rat cortical neurons

2010 ◽  
Vol 68 ◽  
pp. e81
Author(s):  
Mitsuru Ishikawa ◽  
Naoki Nishijima ◽  
Hiroyuki Sakagami ◽  
Kunihiro Tsuchida ◽  
Masaaki Tsuda ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Transcriptional Activity ◽  
Dendritic Morphology ◽  
Actin Binding ◽  
Rat Cortical Neurons

scholarly journals Scapinin-induced Inhibition of Axon Elongation Is Attenuated by Phosphorylation and Translocation to the Cytoplasm

2011 ◽  
Vol 286 (22) ◽  
pp. 19724-19734 ◽  
Author(s):  
Hovik Farghaian ◽  
Yu Chen ◽  
Ada W. Y. Fu ◽  
Amy K. Y. Fu ◽  
Jacque P. K. Ip ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Brain Cortex ◽  
Inhibitory Effect ◽  
Adult Brain ◽  
Actin Binding ◽  
Mutant Form ◽  
Axon Elongation ◽  
Rat Cortical Neurons ◽  
The Brain

Scapinin is an actin- and PP1-binding protein that is exclusively expressed in the brain; however, its function in neurons has not been investigated. Here we show that expression of scapinin in primary rat cortical neurons inhibits axon elongation without affecting axon branching, dendritic outgrowth, or polarity. This inhibitory effect was dependent on its ability to bind actin because a mutant form that does not bind actin had no effect on axon elongation. Immunofluorescence analysis showed that scapinin is predominantly located in the distal axon shaft, cell body, and nucleus of neurons and displays a reciprocal staining pattern to phalloidin, consistent with previous reports that it binds actin monomers to inhibit polymerization. We show that scapinin is phosphorylated at a highly conserved site in the central region of the protein (Ser-277) by Cdk5 in vitro. Expression of a scapinin phospho-mimetic mutant (S277D) restored normal axon elongation without affecting actin binding. Instead, phosphorylated scapinin was sequestered in the cytoplasm of neurons and away from the axon. Because its expression is highest in relatively plastic regions of the adult brain (cortex, hippocampus), scapinin is a new regulator of neurite outgrowth and neuroplasticity in the brain.

Actin-binding coactivator MKL is involved in a novel signaling pathway for activin-regulated dendritic complexity of rat cortical neurons

2011 ◽  
Vol 71 ◽  
pp. e333
Author(s):  
Mitsuru Ishikawa ◽  
Naoki Nishijima ◽  
Hiroyuki Sakagami ◽  
Kunihiro Tsuchida ◽  
Miho Mizukoshi ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Cortical Neurons ◽  
Actin Binding ◽  
Rat Cortical Neurons ◽  
Dendritic Complexity

The Protective Effects of Jatrorrhizine on β-Amyloid (25-35)-Induced Neurotoxicity in Rat Cortical Neurons

2013 ◽  
Vol 11 (8) ◽  
pp. 1030-1037 ◽  
Author(s):  
Tao Luo ◽  
Wei Jiang ◽  
Yan Kong ◽  
Sheng Li ◽  
Feng He ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Protective Effects ◽  
Rat Cortical Neurons ◽  
Β Amyloid

scholarly journals Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Thu T. Duong ◽  
James Lim ◽  
Vidyullatha Vasireddy ◽  
Tyler Papp ◽  
Hung Nguyen ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Transgene Expression ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Cell Types ◽  
Egfp Expression ◽  
Cell Type ◽  
Egfp Gene ◽  
Rat Cortical Neurons

Recombinant adeno-associated virus (rAAV), produced from a nonpathogenic parvovirus, has become an increasing popular vector for gene therapy applications in human clinical trials. However, transduction and transgene expression of rAAVs can differ acrossin vitroand ex vivo cellular transduction strategies. This study compared 11 rAAV serotypes, carrying one reporter transgene cassette containing a cytomegalovirus immediate-early enhancer (eCMV) and chicken beta actin (CBA) promoter driving the expression of an enhanced green-fluorescent protein (eGFP) gene, which was transduced into four different cell types: human iPSC, iPSC-derived RPE, iPSC-derived cortical, and dissociated embryonic day 18 rat cortical neurons. Each cell type was exposed to three multiplicity of infections (MOI: 1E4, 1E5, and 1E6 vg/cell). After 24, 48, 72, and 96 h posttransduction, GFP-expressing cells were examined and compared across dosage, time, and cell type. Retinal pigmented epithelium showed highest AAV-eGFP expression and iPSC cortical the lowest. At an MOI of 1E6 vg/cell, all serotypes show measurable levels of AAV-eGFP expression; moreover, AAV7m8 and AAV6 perform best across MOI and cell type. We conclude that serotype tropism is not only capsid dependent but also cell type plays a significant role in transgene expression dynamics.

Protection of amyloid β protein (25–35)-induced neurotoxicity by methanol extract of Smilacis chinae rhizome in cultured rat cortical neurons

2006 ◽  
Vol 106 (2) ◽  
pp. 230-237 ◽  
Author(s):  
Ju Yeon Ban ◽  
Soon Ock Cho ◽  
Sang Bum Koh ◽  
Kyung-Sik Song ◽  
KiWhan Bae ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Methanol Extract ◽  
Amyloid Β ◽  
Amyloid Β Protein ◽  
Β Protein ◽  
Rat Cortical Neurons

Mechanisms of ammonia-induced cell death in rat cortical neurons: Roles of NMDA receptors and glutathione

2005 ◽  
Vol 47 (1-2) ◽  
pp. 51-57 ◽  
Author(s):  
A KLEJMAN ◽  
M WEGRZYNOWICZ ◽  
E SZATMARI ◽  
B MIODUSZEWSKA ◽  
M HETMAN ◽  
...  
Keyword(s):  
Cell Death ◽  
Nmda Receptors ◽  
Cortical Neurons ◽  
Rat Cortical Neurons

3,4-Dihydroxybenzoic acid from Smilacis chinae rhizome protects amyloid β protein (25–35)-induced neurotoxicity in cultured rat cortical neurons

2007 ◽  
Vol 420 (2) ◽  
pp. 184-188 ◽  
Author(s):  
Ju Yeon Ban ◽  
Soon Ock Cho ◽  
So-Young Jeon ◽  
KiHwan Bae ◽  
Kyung-Sik Song ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Amyloid Β ◽  
Amyloid Β Protein ◽  
Dihydroxybenzoic Acid ◽  
Β Protein ◽  
Rat Cortical Neurons
Sign in / Sign up

Export Citation Format

Share Document