Superior infectivity of the fiber chimeric oncolytic adenoviruses Ad5/35 and Ad5/3 over Ad5-delta-24-RGD in primary glioma cultures

A dual pH- and temperature-responsive physically crosslinked and injectable hydrogel system was developed for efficient and long-term delivery of oncolytic adenoviruses (Ads).

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Oncolytic Adenoviruses for the Treatment of Human Cancer: Focus on Translational and Clinical Data

Molecular Pharmaceutics ◽

10.1021/mp100219n ◽

2010 ◽

Vol 8 (1) ◽

pp. 12-28 ◽

Cited By ~ 77

Author(s):

Sari Pesonen ◽

Lotta Kangasniemi ◽

Akseli Hemminki

Keyword(s):

Clinical Data ◽

Human Cancer ◽

Oncolytic Adenoviruses

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Treatment of metastatic renal cancer with capsid-modified oncolytic adenoviruses

Molecular Cancer Therapeutics ◽

10.1158/1535-7163.mct-07-0176 ◽

2007 ◽

Vol 6 (10) ◽

pp. 2728-2736 ◽

Cited By ~ 31

Author(s):

K. Guse ◽

T. Ranki ◽

M. Ala-Opas ◽

P. Bono ◽

M. Sarkioja ◽

...

Keyword(s):

Renal Cancer ◽

Metastatic Renal Cancer ◽

Oncolytic Adenoviruses

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Oncolytic Adenoviruses in Cancer Treatment

Biomedicines ◽

10.3390/biomedicines2010036 ◽

2014 ◽

Vol 2 (1) ◽

pp. 36-49 ◽

Cited By ~ 21

Author(s):

Ramon Alemany

Keyword(s):

Cancer Treatment ◽

Oncolytic Adenoviruses

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High-dose vitamin C synergizes with oncolytic adenoviruses against tumor by enhancing immunogenic tumor cell death and reprogramming tumor immune microenvironment

Molecular Therapy ◽

10.1016/j.ymthe.2021.09.015 ◽

2021 ◽

Author(s):

Jinhu Ma ◽

Chunxue Zhang ◽

Gang Shi ◽

Dan Yue ◽

Yongheng Shu ◽

...

Keyword(s):

Cell Death ◽

Vitamin C ◽

Tumor Cell ◽

High Dose ◽

Tumor Cell Death ◽

Immune Microenvironment ◽

Oncolytic Adenoviruses ◽

Tumor Immune Microenvironment ◽

Immunogenic Tumor Cell ◽

High Dose Vitamin

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Antimigratory effect of berberine in T98G, U87MG and primary glioma cell culture.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e15045 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e15045-e15045

Author(s):

Irina V. Mezhevova ◽

Svetlana Yu. Filippova ◽

Sofia V. Timofeeva ◽

Anastasia O. Sitkovskaya ◽

Tatiana V. Shamova ◽

...

Keyword(s):

Cell Culture ◽

Cell Lines ◽

Cell Cultures ◽

Glioma Cell ◽

Glioma Cells ◽

Surgical Removal ◽

Experimental Sample ◽

Wound Area ◽

Migratory Activity ◽

Primary Glioma

e15045 Background: Berberine is an alkaloid compound with a structure that is highly similar to that of intercalating agents. It affects numerous cell signaling pathways and is widely studied as potential anticancer drug. It is known that berberine affects cancer cells migration through metalloproteinase-2 inhibition, but this effect was never studied on glioma cells. Anti-migratory drugs are of special interest in brain cancer therapy since glioma's highly invasive nature makes total surgical removal of tumor practically impossible. The aim of the study was to evaluate berberine anti-migratory activity on glioma cells. Methods: Cell migration capacity of T98G and U87MG cell lines, as well as primary glioma cell culture established in our laboratory, was assessed via standard wound healing assay with automated image acquisition and analysis on Lionheart FX (BioTek) cell imager. Prior to assay setting up cell cultures were maintained in DMEM medium with L-glutamine (1 μM) (Gibco) and 10% FBS (Gibco) at 37C0 and 5.0% CO2. Cells were seeded at 250 000 cells per well on 24-well plates and incubated overnight in order to attach to plate bottom. After that a vertical wound was made manually in each well, and berberine was added to experimental wells to final concentration 50 mg/L. Plates with cells were continuously incubated and photographed in cell imager at 37C0 and 5.0% CO2. The extent of cells migration was measured as the percent of wound area decrease after 24 hours of incubation in relation to starting time point. Data are given as: Mean ± 95% confidence interval. Results: In our study we berberine exhibited anti-migratory activity in all cell cultures under study. In rather fast growing primary cell culture wound area decrease was 99.23%±0.62% in control sample and 91.75%±0.28% in experimental sample. The difference was small but significant at p < 0.001 level (df = 30). Popular permanent glioma cell lines T98G and U87MG showed more prominent decrease in studied parameter with higher degree of variance at the same time. In T98G wound area decrease was 71.6%±12.3% in control and 48.8%± 7.6% in experimental samples after 24 hours of cultivation in presence of 50 mg/L berberine. While U87MG demonstrated 60.28%±5.13% and 37.5%± 8.34% wound area decrease accordingly. The obtained difference between control and experimental groups in permanent cell cultures was statistically significant at the 0.05 level (df = 30). Conclusions: Our preliminary research proved berberine to be potent anti-migratory agent in glioma treatment. Further investigations are needed to evaluate its ability to inhibit glioma cell expansion in vivo.

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Unleashing the Full Potential of Oncolytic Adenoviruses against Cancer by Applying RNA Interference: The Force Awakens

Cells ◽

10.3390/cells7120228 ◽

2018 ◽

Vol 7 (12) ◽

pp. 228 ◽

Cited By ~ 4

Author(s):

Tereza Brachtlova ◽

Victor van Beusechem

Keyword(s):

Rna Interference ◽

Immune Responses ◽

Oncolytic Viruses ◽

Full Potential ◽

Anticancer Efficacy ◽

Host Interactions ◽

Oncolytic Adenoviruses ◽

Cellular Factors ◽

Immunotherapy Of Cancer ◽

Cellular Pathways

Oncolytic virus therapy of cancer is an actively pursued field of research. Viruses that were once considered as pathogens threatening the wellbeing of humans and animals alike are with every passing decade more prominently regarded as vehicles for genetic and oncolytic therapies. Oncolytic viruses kill cancer cells, sparing healthy tissues, and provoke an anticancer immune response. Among these viruses, recombinant adenoviruses are particularly attractive agents for oncolytic immunotherapy of cancer. Different approaches are currently examined to maximize their therapeutic effect. Here, knowledge of virus–host interactions may lead the way. In this regard, viral and host microRNAs are of particular interest. In addition, cellular factors inhibiting viral replication or dampening immune responses are being discovered. Therefore, applying RNA interference is an attractive approach to strengthen the anticancer efficacy of oncolytic viruses gaining attention in recent years. RNA interference can be used to fortify the virus’ cancer cell-killing and immune-stimulating properties and to suppress cellular pathways to cripple the tumor. In this review, we discuss different ways of how RNA interference may be utilized to increase the efficacy of oncolytic adenoviruses, to reveal their full potential.

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