Intratumor genomic heterogeneity correlates with histological grade of advanced oral squamous cell carcinoma

Oral Oncology ◽  
2006 ◽  
Vol 42 (7) ◽  
pp. 740-744 ◽  
Author(s):  
Xinhong Wang ◽  
Mingwen Fan ◽  
Xinming Chen ◽  
Shuozhi Wang ◽  
Mohd Jamal Alsharif ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histological Grade

scholarly journals Proline-Dependent Induction of Apoptosis in Oral Squamous Cell Carcinoma (OSCC)—The Effect of Celecoxib

Cancers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 136 ◽  
Author(s):  
Natalia Tołoczko-Iwaniuk ◽  
Dorota Dziemiańczyk-Pakieła ◽  
Katarzyna Celińska-Janowicz ◽  
Ilona Zaręba ◽  
Agnieszka Klupczyńska ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histological Grade ◽  
Clinical Stage ◽  
High Morbidity ◽  
Proline Metabolism ◽  
Proline Concentration

Background: Oral squamous cell carcinoma remains a significant worldwide public health challenge, associated with high morbidity and mortality. Treatment of this type of cancer lacks effective medication. Moreover, there are very few specific biomarkers that are useful in early diagnosis or treatment optimisation. Proline metabolism may prove to be of importance in the search for new treatment modalities. Methods: To evaluate the significance of proline metabolism in the development of oral cancer, proline concentration was assessed in oral cancer tissue and normal oral mucosa. The results were compared to the clinical stage and histological grade of the tumours. Moreover, the expression of proteins involved in proline metabolism via proline dehydrogenase/oxidase (PRODH/POX, PPARγ, HIF1-α) was determined. In the next stage of the study, conducted on cell lines of tongue cancer treated with celecoxib, the aforementioned factors involved in proline metabolism were evaluated. Cellular viability and cell proliferation, as well as apoptosis, were also assessed. Results: Our research results indicate that a high intracellular proline concentration and expression of factors involved in its metabolism correlate with the clinical stage and histological grade of oral cancer. Moreover, we are the first researchers to demonstrate that celecoxib can affect proline metabolism, causing an increase in pro-apoptotic factors (PRODH/POX, PPARγ), reducing the expression of HIF-1α and activating apoptosis. Conclusions: Proline metabolism, due to its involvement in the process of apoptosis, can be of great importance in anticancer therapy. It appears that celecoxib, which influences the PRODH/POX pathway, may be a promising therapeutic compound in oral cancer treatment.

scholarly journals Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Geovanni Pereira Mitre ◽  
Karolyny Martins Balbinot ◽  
André Luis Ribeiro Ribeiro ◽  
Maria Sueli da Silva Kataoka ◽  
Sérgio de Melo Alves Júnior ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Statistical Difference ◽  
Histological Grade ◽  
Clinical Stage ◽  
Malignant Neoplasm ◽  
Complex Events ◽  
Related Proteins

Abstract Background Oral squamous cell carcinoma (OSCC) is the most relevant malignant neoplasm among all head and neck tumours due to its high prevalence and unfavourable prognosis. Tumour invasion and metastasis that affect prognosis are result of a set of complex events that cells with invasive potential use to spread to other regions. These cells use several mechanisms to invade tissues, including a type of finger-like membrane protrusion called invadopodia. This study aims to investigate the immunoexpression of invaopodia related-proteins TKs5, cortactin, TKs4 and MT1-MMP in OSCC and correlate it to clinicopathological data. Methods An immunohistochemical evaluation of fifty cases of OSCCs and 20 cases of oral mucosa (OM) were assessed. The expression of invadopodia proteins were analysed in comparison to normal tissue (OM) and correlated to different clinical-stage and histological grade of OSCC. Results TKs5, cortactin, TKs4 and MT1-MMP were significantly overexpressed in OSCC when compared to OM (p < 0.0001). Among tumour stages, TKs5 showed a statistical difference in immunolabelling between stage I and III (p = 0.026). Cortactin immunolabelling was statistically higher in grade I than in grade II and III. No differences were seen on TKs4 expression based on tumour staging or grading. MT1-MMP was higher expressed and showed statistical difference between stages I and III and grades I compared to II and III. Conclusions The invadopodia related-proteins were found to be overexpressed in OSCC when compared to OM, suggesting invadopodia formation and activity. Besides overexpressed in OSCC, cortactin, TKs4 and TKs5 showed no or ambiguous differences in protein expression when compared among clinical-stages or histological grades groups. Conversely, the expression of MT1-MMP increased in advanced stages and less differentiated tumours, suggesting MT1-MMP expression as a promising prognostic marker in OSCC.

scholarly journals Oral squamous cell carcinoma in patients less than 40 years in a Nigerian population

2020 ◽  
Vol 9 (2) ◽  
pp. 40-45
Author(s):  
A.O Lawal ◽  
A.O Adisa ◽  
O.A Effiom
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histological Grade ◽  
Teaching Hospitals ◽  
Female Ratio ◽  
Case File ◽  
Male Female Ratio ◽  
Well Differentiated

Objectives: Oral cancer ranks amongst the sixth to eight most common cancers worldwide and exhibits a great variation in incidence among countries. Oral squamous cell carcinoma (OSCC) is rare in individuals less than 40 years of age, with occurrences averaging 4 to 6% in people less than 40 years. The aim of this study was to review the clinical and pathological characteristics of OSCC cases in patients less than 40 years of age in five tertiary health facilities in Nigeria.Methods:All OSCC in the period from 1970 to 2015 from case file records and biopsy reports were retrieved from the records of the five teaching hospitals, to obtain age, gender, location and histologic grades.Results: Ninety-seven (17.4%) cases of OSCC were diagnosed in patients less than 40 years of age. These included 58 males and 38 females giving a male: female ratio of 1.5:1. The mandibular mucosa with 27 (28.7%) cases was the most common site followed by the maxillary mucosa with 24 (25.5%) and palate with 14 (14.4%) cases. Only 5 (5.3 %) cases of OSCC occurred in the tongue. The well differentiated OSCC was the most common histological grade accounting for 48 (50.0 %) cases while the moderately differentiated and poorly differentiated OSCC accounted for 31 (32.3 %) and 17 (17.7 %) cases respectively.Conclusion: OSCC was relatively more common in patients less than 40 years of age in this study than those of previous studies from other regions and OSCC in patients less than 40 years of age was relatively rare in the tongue when compared with similar cohorts from other continents. Key words: oral squamous cell carcinoma; age less than 40; tongue; mandibular mucosa, Nigeria

scholarly journals The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 8 (2) ◽  
pp. 273 ◽  
Author(s):  
Miyako Kurihara-Shimomura ◽  
Tomonori Sasahira ◽  
Hiroyuki Shimomura ◽  
Chie Nakashima ◽  
Tadaaki Kirita
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Histological Grade ◽  
Oral Epithelium ◽  
Mesenchymal Transition ◽  
The Relationship

Background: The relationship between miR-29b-1-5p and c-Met proto-oncogene in oral squamous cell carcinoma (OSCC) remains to be investigated. This study aimed to reveal the role of miR-29b-1-5p in the pathogenesis of OSCC using molecular and biological analyses. Methods: We investigated the expression of miR-29b-1-5p, c-Met, and markers of the epithelial-mesenchymal transition (EMT) in the tissues of 49 patients with OSCC and in human OSCC cells with different tumorigenicity. Further, we determined the effects of miR-29b-1-5p on the phenotypes of OSCC cell lines. Results: The expression levels of miR-29b-1-5p in most patients with OSCC were higher than those of the normal oral epithelium. In OSCC, upregulation of miR-29b-1-5p significantly correlated with histological grade, the EMT, and the immunohistochemical grade, indicated by c-Met expression. The prognosis was poor for patients with miR-29b-1-5p expression and coexpression of miR-29b-1-5p and c-Met. In OSCC cells exhibiting the EMT phenotype, knockdown of miR-29b-1-5p suppressed the EMT, which was recovered by enforced expression of c-Met. Further, the mRNA encoding cadherin 1 (CDH1) was a direct target of miR-29b-1-5p. Conclusions: Our results suggest that miR-29b-1-5p acts as an oncogenic miRNA that synergizes with c-Met to induce the EMT of OSCC cells.

Analysis of influencing factors and prognosis of early postoperative recurrence, secondary tumor and metastasis of oral squamous cell carcinoma

2021 ◽  
Vol 67 (3) ◽  
pp. 118-124
Author(s):  
Haiyang Tang ◽  
Feiyun Hu ◽  
Xinying Li ◽  
Hongjie Song
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Influencing Factors ◽  
Squamous Cell ◽  
Clinical Condition ◽  
Histological Grade ◽  
Postoperative Recurrence ◽  
Secondary Tumor ◽  
Risk Of Death

Oral squamous cell carcinoma (OSCC) is one of the most common cancers in the world and it accounts for more than 90% of oral cancers. In this study, we tried to estimate the risk of early postoperative recurrence, secondary tumor and metastasis of OSCC to predict the patient's prognosis according to its clinical condition to help increase their survival by screening high-risk patients. 153 patients with OSCC who were over 40 years of age were studied during 1985-2020. The influencing factors included gender, race, stage of tumor progression, treatment method, histological grade and tumor location, date of diagnosis and death, which were analyzed by the Markov multi-state model. Also, their saliva was sampled to determine the amount of Matrix Metalloproteinase13 (MMP13). Following-up of patients for 60 months showed that one year after the end of treatment, the probability of death was almost the same for patients with early postoperative recurrence or secondary tumor, but after 5 years, patients with early postoperative recurrence are at higher risk of death. Also, the MMP13 amount in the saliva of patients showed that high levels of MMP13 belonged to metastasis of OSCC than early postoperative recurrence and secondary tumor. Therefore, patients with more amount of MMP13 are more involved in metastasis than early postoperative recurrence and secondary tumor. Approximate knowledge of OSCC patients' next state and time according to their clinical condition can be one of the ways of timely diagnosis and treatment and thus reduce their mortality rate.

scholarly journals Different tumour grading in smokeless tobacco (Naswar) user and non-user in squamous cell carcinoma.

2020 ◽  
Vol 27 (05) ◽  
pp. 1054-1058
Author(s):  
Amna Sarfaraz ◽  
Sahd Rashid ◽  
Muhammad Irshad ◽  
Maria Tasneem Khattak ◽  
Hina Shabir
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histological Grade ◽  
Oral Pathology ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Tumour Grading ◽  
History Of

Objectives: To determine the relationship of Naswar with different tumour grading in squamous cell carcinoma. Study Design: Comparative cross-sectional study using non-probability, consecutive sampling. Setting: Department of Oral Pathology, Rehman College of Dentistry, Peshawar, Khyber Pakhtunkhwa, Pakistan. Period: January 2017 till June 2017. Material & Methods: 60 cases of oral squamous cell carcinoma were included. Half the cases (30) had a history of Naswar use while the other half (30) had no history of Naswar use. Data was analysed using SPSS v 22. Results: This study included 60% male and 40% female cases, ranging from 40 years to 80 years (mean = 57.6 + 11 years). Majority of patients 80% had well differentiated squamous cell carcinoma, while the rest (20%) had poorly differentiated. Distribution of male and female across both groups was equal (p 0.598). Both the groups were comparable in terms of grade of tumour (p 0.519). Conclusion: Naswar use was not associated with any particular histological grade of oral squamous cell carcinoma in our study. However, further research is needed to explore this association.

scholarly journals The IMMUNOHISTOCHEMICAL EXPRESSION OF NOVEL B7-H6 IN ORAL SQUAMOUS CELL CARCINOMA AND CORRELATION WITH HISTOLOGICAL DIFFERENTIATION AND CLINICOPATHOLOGICAL PARAMETERS

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Taqia Fauzia ◽  
Serajuddaula Syed ◽  
Faraz Ahmed Baig ◽  
Maheen Zakaria
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Histological Grade ◽  
Immunohistochemical Analysis ◽  
Clinicopathological Characteristics ◽  
Clinicopathological Parameters ◽  
P Value ◽  
Immunohistochemical Expression

B7-H6 is a new immune checkpoint molecule related to B7 family; however, little research has been performed to determine its expression among tumors. In this study, we investigated the immunohistochemical expression of B7-H6 protein in 141 biopsy proven oral squamous cell carcinoma tissues. The specimens were subjected for immunohistochemical analysis to evaluated B7-H6 expression and was graded according to the intensity of cytoplasm and membrane staining. The distribution of B7-H6 expression was also correlated with different clinicopathological parameters of oral squamous cell carcinoma.Overall, 69 out of 141 oral squamous cell carcinoma cases had shown the expression of B7-H6 protein, majority of which belongs to moderately differentiated histological grade. This accounts for strong statistical association of B7-H6 with oral squamous cell carcinoma (P-value=0.001). The distribution of B7-H6 was also observed across broad range of clinicopathological characteristics of disease, however only long term naswar consumers have shown significant statistical difference with B7-H6 expression. We thus conclude that, B7-H6 may represent a new marker for oral squamous cell carcinoma with potential to forecast biological aggressiveness of oral squamous cell carcinoma. Hence, B7-H6 may serve as an immune target for oral squamous cell carcinoma therapy, which needs to evaluated in future studies.

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