Acanthamoeba mauritaniensis genotype T4D: An environmental isolate displays pathogenic behavior

2020 ◽  
Vol 74 ◽  
pp. 102002 ◽  
Author(s):  
Daniel Coronado-Velázquez ◽  
Angélica Silva-Olivares ◽  
Federico Castro-Muñozledo ◽  
Luis Fernando Lares-Jiménez ◽  
Libia Zulema Rodríguez-Anaya ◽  
...  
2021 ◽  
Author(s):  
Lummy Maria Oliveira Monteiro ◽  
Joao Saraiva ◽  
Rodolfo Brizola Toscan ◽  
Peter F Stadler ◽  
Rafael Silva-Rocha ◽  
...  

AbstractTranscription Factors (TFs) are proteins that control the flow of genetic information by regulating cellular gene expression. Here we describe PredicTF, a first platform supporting the prediction and classification of novel bacterial TF in complex microbial communities. We evaluated PredicTF using a two-step approach. First, we tested PredictTF’s ability to predict TFs for the genome of an environmental isolate. In the second evaluation step, PredicTF was used to predict TFs in a metagenome and 11 metatranscriptomes recovered from a community performing anaerobic ammonium oxidation (anammox) in a bioreactor. PredicTF is open source pipeline available at https://github.com/mdsufz/PredicTF.


2014 ◽  
Vol 30 (10) ◽  
pp. 2723-2731 ◽  
Author(s):  
Sweety A. Wadhwani ◽  
Utkarsha U. Shedbalkar ◽  
Richa Singh ◽  
Meena S. Karve ◽  
Balu A. Chopade

2010 ◽  
Vol 54 (4) ◽  
pp. 1619-1622 ◽  
Author(s):  
Delphine Girlich ◽  
Laurent Poirel ◽  
Patrice Nordmann

ABSTRACT An Aeromonas allosaccharophila environmental isolate recovered from the Seine River (Paris, France) produced a novel extended-spectrum β-lactamase, PER-6, that shared 92% amino acid identity with the closest ß-lactamase, PER-2. The kinetic properties of PER-6 showed a slightly increased affinity for carbapenems. The bla PER-6 gene was chromosomally located and bracketed by non-transposon-related structures.


2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto

Listeria monocytogenesis a foodborne pathogen that causes severe illness. Thus, ongoing efforts at real-time whole-genome sequencing are of utmost importance. However, it is also important that retrospective analyses that place these data into context be performed. Here, we present the genome sequence of strain HPB2088, which was collected in 1994.


2000 ◽  
Vol 182 (15) ◽  
pp. 4241-4248 ◽  
Author(s):  
Patricia C. Brzostowicz ◽  
Katharine L. Gibson ◽  
Stuart M. Thomas ◽  
Mary Sue Blasko ◽  
Pierre E. Rouvière

ABSTRACT The technique of mRNA differential display was used to identify simultaneously two metabolic genes involved in the degradation of cyclohexanone in a new halotolerant Brevibacteriumenvironmental isolate. In a strategy based only on the knowledge that cyclohexanone oxidation was inducible in this strain, the mRNA population of cells exposed to cyclohexanone was compared to that of control cells using reverse transcription-PCR reactions primed with a collection of 81 arbitrary oligonucleotides. Three DNA fragments encoding segments of flavin monooxygenases were isolated with this technique, leading to the identification of the genes of two distinct cyclohexanone monooxygenases, the enzymes responsible for the oxidation of cyclohexanone. Each monooxygenase was expressed in Escherichia coli and characterized. This work validates the application of mRNA differential display for the discovery of new microbial metabolic genes.


PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132392 ◽  
Author(s):  
Laura Newsome ◽  
Katherine Morris ◽  
Jonathan. R. Lloyd

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Shatavia S. Morrison ◽  
Heta P. Desai ◽  
Jeffrey W. Mercante ◽  
Pascal Lapierre ◽  
Brian H. Raphael ◽  
...  

We report here the complete genome sequences of three Legionella pneumophila isolates that are associated with a Legionnaires’ disease outbreak in New York in 2012. Two clinical isolates (D7630 and D7632) and one environmental isolate (D7631) were recovered from this outbreak. A single isolate-specific virulence gene was found in D7632. These isolates were included in a large study evaluating the genomic resolution of various bioinformatics approaches for L. pneumophila serogroup 1 isolates.


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