Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana

2005 ◽  
Vol 66 (8) ◽  
pp. 859-867 ◽  
Author(s):  
Marta de Torres Zabala ◽  
Murray Grant ◽  
Atle M. Bones ◽  
Richard Bennett ◽  
Yin Sze Lim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Over Expression ◽  
Epithiospecifier Protein

scholarly journals Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition

2021 ◽  
Vol 22 (11) ◽  
pp. 5550
Author(s):  
Hongshun Li ◽  
Yiwei Luo ◽  
Bi Ma ◽  
Jianqiong Hu ◽  
Zhiyuan Lv ◽  
...  
Keyword(s):  
Phase Transition ◽  
Arabidopsis Thaliana ◽  
Woody Plants ◽  
Luciferase Reporter ◽  
Vegetative Phase ◽  
Promoter Sequences ◽  
Over Expression ◽  
Degradome Sequencing ◽  
Pathway Expression ◽  
Reporter Assays

The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine MnSPLs were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants.

Over-Expression of PmHSP17.9 in Transgenic Arabidopsis thaliana Confers Thermotolerance

2016 ◽  
Vol 34 (5) ◽  
pp. 899-908 ◽  
Author(s):  
Xue-Li Wan ◽  
Jie Yang ◽  
Xiao-Bai Li ◽  
Qiao Zhou ◽  
Cong Guo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Arabidopsis ◽  
Over Expression ◽  
Transgenic Arabidopsis Thaliana

Over-expression of an AT-hook gene, AHL22, delays flowering and inhibits the elongation of the hypocotyl in Arabidopsis thaliana

2009 ◽  
Vol 71 (1-2) ◽  
pp. 39-50 ◽  
Author(s):  
Chaowen Xiao ◽  
Fulu Chen ◽  
Xuhong Yu ◽  
Chentao Lin ◽  
Yong-Fu Fu
Keyword(s):  
Arabidopsis Thaliana ◽  
Over Expression

scholarly journals Variation in the expression of a transmembrane protein influences cell growth in Arabidopsis thaliana petals by altering auxin responses

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Charlotte N. Miller ◽  
Jack Dumenil ◽  
Fu Hao Lu ◽  
Caroline Smith ◽  
Neil McKenzie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Genetic Variation ◽  
Cell Growth ◽  
Plant Growth ◽  
Cell Size ◽  
Natural Populations ◽  
Organ Size ◽  
Substantial Contribution ◽  
Over Expression

Abstract Background The same species of plant can exhibit very diverse sizes and shapes of organs that are genetically determined. Characterising genetic variation underlying this morphological diversity is an important objective in evolutionary studies and it also helps identify the functions of genes influencing plant growth and development. Extensive screens of mutagenised Arabidopsis populations have identified multiple genes and mechanisms affecting organ size and shape, but relatively few studies have exploited the rich diversity of natural populations to identify genes involved in growth control. Results We screened a relatively well characterised collection of Arabidopsis thaliana accessions for variation in petal size. Association analyses identified sequence and gene expression variation on chromosome 4 that made a substantial contribution to differences in petal area. Variation in the expression of a previously uncharacterised gene At4g16850 (named as KSK) had a substantial role on variation in organ size by influencing cell size. Over-expression of KSK led to larger petals with larger cells and promoted the formation of stamenoid features. The expression of auxin-responsive genes known to limit cell growth was reduced in response to KSK over-expression. ANT expression was also reduced in KSK over-expression lines, consistent with altered floral identities. Auxin responses were reduced in KSK over-expressing cells, consistent with changes in auxin-responsive gene expression. KSK may therefore influence auxin responses during petal development. Conclusions Understanding how genetic variation influences plant growth is important for both evolutionary and mechanistic studies. We used natural populations of Arabidopsis thaliana to identify sequence variation in a promoter region of Arabidopsis accessions that mediated differences in the expression of a previously uncharacterised membrane protein. This variation contributed to altered auxin responses and cell size during petal growth.

Ecotopic over‐expression of PoCHS from Paeonia ostii altered the fatty acids composition and content in Arabidopsis thaliana

2020 ◽  
Author(s):  
Li‐Ping Peng ◽  
Qing Hao ◽  
Si‐Qi Men ◽  
Xi‐Ruo Wang ◽  
Wen‐Yuan Huang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Fatty Acids Composition ◽  
Over Expression
Sign in / Sign up

Export Citation Format

Share Document