Enhanced production of phenylethanoid glycosides by precursor feeding to cell culture of Cistanche deserticola

Established biotechnologies overcome cost handicaps, particularly aqueous process dilution, through unique advantages. Ancient fermentations confer psychopharmacological, palatable or nutritional qualities on drinks and foods. Modern biotechnologies depend on bacterial versatility (sewage disposal), enzyme specificity (hydrolases), high value of complex products (antibiotics, vaccines), chirality (amino acids), high yield (citric acid), or, rarely, process intensity (glucose isomerase). Advances in recombinant DNA have already given valuable human proteins. Numerous new targets include hormones, blood proteins and antibodies. Production techniques will include cell fusion and animal cell culture, and new hosts, some eukaryotic, for human genes. Enhanced production of cells, organelles and enzymes will follow, partly to take advantage of the industrial potential of immobilization. Later still, improved engineering and new biological processes should allow competitive production of fine chemicals, protein and other foods, bulk chemicals and fuels. Some products will require simultaneous change and advance in agriculture: interspecific genetic manipulation, plant cell culture and other modern developments will facilitate these.

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Precursor-feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum

Process Biochemistry ◽

10.1016/j.procbio.2007.01.010 ◽

2007 ◽

Vol 42 (5) ◽

pp. 899-903 ◽

Cited By ~ 17

Author(s):

Meng-Hsin Lee ◽

Jing-Jy Cheng ◽

Cha-Yui Lin ◽

Yi-Jyun Chen ◽

Mei-Kuang Lu

Keyword(s):

Cell Culture ◽

Feeding Strategy ◽

Precursor Feeding ◽

A Cell ◽

Solanum Lyratum

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Effects of feeding tyrosine and phenylalanine on the accumulation of phenylethanoid glycosides to Cistanche deserticola cell suspension culture

Chinese Journal of Natural Medicines ◽

10.1016/s1875-5364(14)60045-5 ◽

2014 ◽

Vol 12 (5) ◽

pp. 367-372 ◽

Cited By ~ 2

Author(s):

Gao-Sheng HU ◽

Jing-Ming JIA ◽

Doh Hoon Kim

Keyword(s):

Suspension Culture ◽

Cell Suspension ◽

Cell Suspension Culture ◽

Phenylethanoid Glycosides ◽

Cistanche Deserticola

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Enhanced Production of Bryonolic Acid in Trichosanthes cucumerina L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities

Plants ◽

10.3390/plants9060709 ◽

2020 ◽

Vol 9 (6) ◽

pp. 709 ◽

Cited By ~ 1

Author(s):

Pornpatsorn Lertphadungkit ◽

Jiraphong Suksiriworapong ◽

Veena Satitpatipan ◽

Supaart Sirikantaramas ◽

Amaraporn Wongrakpanich ◽

...

Keyword(s):

Cell Culture ◽

Medicinal Plant ◽

Cell Cultures ◽

Biological Activities ◽

Dry Weight ◽

Cell Suspensions ◽

Root Extracts ◽

Enhanced Production ◽

Dmso Treatment ◽

Mcf 7

Bryonolic acid is a triterpenoid compound found in cucurbitaceous roots. Due to its biological activities, this compound gets more attention to improve production. Herein, we carried out efficient ways with high bryonolic acid productions from Trichosanthes cucumerina L., a Thai medicinal plant utilizing plant cell cultures. The results showed that calli (24.65 ± 1.97 mg/g dry weight) and cell suspensions (15.69 ± 0.78 mg/g dry weight) exhibited the highest bryonolic acid productions compared with natural roots (approximately 2 mg/g dry weight). In the presence of three elicitors (methyl jasmonate, yeast extract, and chitosan), cell suspensions treated with 1 mg/mL of chitosan for eight days led to higher bryonolic acid contents (23.56 ± 1.68 mg/g dry weight). Interestingly, cell culture and root extracts with high bryonolic acid contents resulted in significantly higher percent cell viabilities than those observed under control (1% v/v DMSO) treatment in Saos-2 and MCF-7 cells. The present study indicated that T. cucumerina L. cell cultures are alternative and efficient to produce the biologically important secondary metabolite.

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