Effects of manganese and Bacillus subtilis on the reproductive performance, egg quality, antioxidant capacity, and gut microbiota of breeding geese during laying period

This study aimed to investigate the effects of dietary yeast culture (YC) supplementation on egg production, egg quality, reproductive performance, immune functions, antioxidant capacity, and intestinal microbial structure of aged hens. A total of 224 Hy-Line Brown layers (54 weeks old) were randomly assigned to two dietary treatments. The control group was fed a basal diet and the YC group was supplemented with YC at 2.0 g/kg of their diet. Each group had seven replicates with 16 hens each. The study was conducted over a period of 8 weeks. Results indicated that YC addition had no significant effect on laying performance. However, it significantly improved egg quality and hatching rate, enhanced ileum crude fat digestibility, increased the serum parameters of lysozyme (LZM) and total antioxidation capacity (T-AOC) (P < 0.05), and reduced serum aspartate aminotransferase (AST) levels (P < 0.05). Using 16S rRNA analysis, we found that addition of YC significantly altered ileum microbial composition. Linear discriminant analysis of effect size (LEfSe) showed significant enrichment of Bacilli and Lactobacilli in the YC group. PICRUSt analysis of the ileal microbiota found that glutathione metabolism, ubiquinone, and other terpenoid-quinone biosynthesis and lipopolysaccharide biosynthesis protein pathways were highly enriched in the YC group compared with the basal diet group. In summary, the addition of YC can improve egg quality, immune functions, antioxidant capacity, reproduction efficiency, and digestive absorption by increasing the abundance of Lactobacilli and Bacilli. Furthermore, it also improves the biosynthesis of lipopolysaccharide proteins, glutathione metabolism, and the synthesis of ubiquinone and other terpenoid-quinone metabolic pathways.

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Effects of Clostridium butyricum, Sodium Butyrate, and Butyric Acid Glycerides on the Reproductive Performance, Egg Quality, Intestinal Health, and Offspring Performance of Yellow-Feathered Breeder Hens

Frontiers in Microbiology ◽

10.3389/fmicb.2021.657542 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yibing Wang ◽

Yang Wang ◽

Xiajing Lin ◽

Zhongyong Gou ◽

Qiuli Fan ◽

...

Keyword(s):

Gut Microbiota ◽

Growth Performance ◽

Butyric Acid ◽

Sodium Butyrate ◽

Reproductive Performance ◽

Egg Quality ◽

Intestinal Morphology ◽

Intestinal Health ◽

Offspring Performance ◽

Breeder Hens

Butyrate has been reported to promote the performance and growth of chickens. The specific roles and efficacy of different sources of butyrate remained unclear. Thus, the present study aimed to investigate and compare the effects of Clostridium butyricum (CB), sodium butyrate (SB), and butyric acid glycerides (tributyrin, BAG) on the reproductive performance, egg quality, intestinal health, and offspring performance of yellow-feathered breeder hens. A total of 300 Lingnan yellow-feathered breeder hens were assigned to five treatment groups: control (CL), 1×108CFU/kg CB (CBL), 1×109CFU/kg CB (CBH), 500mg/kg SB, and 300mg/kg BAG. Results showed that the laying performance and egg quality were increased by CBL, CBH, and BAG. Both CB treatments increased the hatchability of fertilized eggs. Maternal supplementation with both levels of CB significantly elevated the growth performance of offspring. Treatment with CBL, CBH, SB, and BAG all improved the oviduct-related variables and reduced the plasmal antioxidant variables. The CBH, CBL, and BAG treatments also improved the intestinal morphology to different degrees. Jejunal contents of IL-6 were decreased by CBH and BAG, while those of IL-4, IL-6, IL-1β, and IgY were decreased by SB. Transcripts of nutrient transporters in jejunal mucosa were also upregulated by CBH, CBL, and SB treatments and expression of Bcl-2-associated X protein was decreased by CBL, CBH, and BAG. In cecal contents, CBL increased the abundance of Firmicutes and Bacillus, while CBH decreased the abundance of Proteobacteria. Also, the co-occurrence networks of intestinal microbes were regulated by CBH and BAG. In conclusion, dietary inclusion of CB and BAG improved the reproductive parameters, egg quality, and intestinal morphology of breeders. CB also influenced the hatching performance of breeders and growth performance of the offspring, while SB improved the oviduct-related variables. These beneficial effects may result from the regulation of cytokines, nutrient transporters, apoptosis, and gut microbiota; high-level CB had more obvious impact. Further study is needed to explore and understand the correlation between the altered gut microbiota induced by butyrate and the performance, egg quality, intestinal health, and also offspring performance.

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Protective effects of Bacillus subtilis ASAG 216 on growth performance, antioxidant capacity, gut microbiota and tissues residues of weaned piglets fed deoxynivalenol contaminated diets

Food and Chemical Toxicology ◽

10.1016/j.fct.2020.111962 ◽

2021 ◽

Vol 148 ◽

pp. 111962

Author(s):

Ru Jia ◽

Faizan Ahmed Sadiq ◽

Wenbin Liu ◽

Lirong Cao ◽

Zhuoyu Shen

Keyword(s):

Bacillus Subtilis ◽

Gut Microbiota ◽

Antioxidant Capacity ◽

Growth Performance ◽

Protective Effects ◽

Weaned Piglets

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Dietary combined supplementation of iron and Bacillus subtilis enhances reproductive performance, eggshell quality, nutrient digestibility, antioxidant capacity, and hematopoietic function in breeder geese

Poultry Science ◽

10.1016/j.psj.2020.06.077 ◽

2020 ◽

Vol 99 (11) ◽

pp. 6119-6127

Author(s):

Beibei Zhang ◽

Fuliang Sui ◽

Baowei Wang ◽

Yang Wang ◽

Wenli Li

Keyword(s):

Bacillus Subtilis ◽

Antioxidant Capacity ◽

Reproductive Performance ◽

Nutrient Digestibility ◽

Eggshell Quality ◽

Combined Supplementation

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Glycerol Monolaurate Enhances Reproductive Performance, Egg Quality and Albumen Amino Acids Composition in Aged Hens with Gut Microbiota Alternation

Agriculture ◽

10.3390/agriculture10070250 ◽

2020 ◽

Vol 10 (7) ◽

pp. 250 ◽

Cited By ~ 4

Author(s):

Tao Liu ◽

Chuang Li ◽

Yang Li ◽

Fengqin Feng

Keyword(s):

Amino Acids ◽

Gut Microbiota ◽

Sex Hormones ◽

Serum Calcium ◽

Reproductive Performance ◽

Laying Hens ◽

Egg Quality ◽

Total Serum ◽

Control Group ◽

Glycerol Monolaurate

As it is known to all that the laying rate of commercial laying hens is significantly decreased during the late laying period, paralleled with increased feed conversion rate (FCR) and declined egg quality. However, recent studies demonstrate that reproductive performance as well as egg quality in aged hens can be largely improved by feed supplements. Among them, glycerol monolaurate (GML), a monoglyceride of medium chain fatty acids, is regarded as a promising supplement in poultry production. The aim of this experiment was to evaluate the effect of dietary GML on laying performance, serum biochemical indices, egg quality and gut microbiota in hens during 40–64 weeks of age. A total of 378 (40-week-old) Hy-Line Brown laying hens were randomly assigned into three treatments (21 hens/replicate and 6 replicates/treatment). The control group received a basal diet, and the treated groups fed basal diets containing 150 and 300 mg/kg GML. The results revealed that laying rate (p < 0.05) was significantly increased and the FCR (p < 0.05) was reduced in GML-containing groups compared with the control group. The egg quality (including eggshell thickness and strength) was notably improved by GML supplementation at the later late laying period, and the content of total amino acids and flavor amino acids in the albumen were also increased. Inclusion of 300 mg/kg GML in diet increased the levels of laying-related sex hormones (serum follicle stimulating hormone, luteinizing hormone and estradiol, p < 0.05), serum calcium and lipid (total serum cholesterol, triglycerides and low-density lipoprotein cholesterol, p < 0.05). Moreover, the community and microbial function of gut microbiota were notably altered by GML supplementation. These findings revealed that dietary GML improved the reproductive performance and egg quality mainly by stimulating the sex hormones secretion, serum calcium metabolism and nutrients utilization, which provided us a new approach to simultaneously promote the egg production and improve the egg quality at aged hens.

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Estimation of dietary tryptophan requirement for laying duck breeders: effects on productive and reproductive performance, egg quality, reproductive organ and ovarian follicle development and serum biochemical indices

Poultry Science ◽

10.1016/j.psj.2021.101145 ◽

2021 ◽

pp. 101145

Author(s):

Y.N. Zhang ◽

D. Ruan ◽

S. Wang ◽

X.B. Huang ◽

K.C. Li ◽

...

Keyword(s):

Reproductive Performance ◽

Egg Quality ◽

Ovarian Follicle ◽

Reproductive Organ ◽

Follicle Development ◽

Ovarian Follicle Development ◽

Serum Biochemical ◽

Biochemical Indices

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Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata

Animals ◽

10.3390/ani11020519 ◽

2021 ◽

Vol 11 (2) ◽

pp. 519

Author(s):

Shajahan Ferosekhan ◽

Serhat Turkmen ◽

Cathaysa Pérez-García ◽

Hanlin Xu ◽

Ana Gómez ◽

...

Keyword(s):

Reproductive Performance ◽

Egg Quality ◽

Sparus Aurata ◽

Genetic Selection ◽

Growth Trait ◽

High Growth ◽

Gilthead Seabream ◽

Commercial Diet ◽

Selection For ◽

Broodstock Diet

Genetic selection in gilthead seabream (GSB), Sparus aurata, has been undertaken to improve the growth, feed efficiency, fillet quality, skeletal deformities and disease resistance, but no study is available to delineate the effect of genetic selection for growth trait on GSB reproductive performance under mass spawning condition. In this study, high growth (HG) or low growth (LG) GSB broodstock were selected to evaluate the sex steroid hormones, sperm, egg quality and reproductive performance under different feeding regime of commercial diet or experimental broodstock diet containing either fish oil (FO) or vegetable oil (VO) based diet. Under commercial diet feeding phase, broodstock selected for either high growth or low growth did not show any significant changes in the egg production per kg female whereas egg viability percentage was positively (p = 0.014) improved by the high growth trait broodstock group. The experimental diet feeding results revealed that both growth trait and dietary fatty acid composition influenced the reproductive performance of GSB broodstock. In the experimental diet feeding phase, we observed high growth trait GSB males produced a higher number of sperm cells (p < 0.001) and also showed a higher sperm motility (p = 0.048) percentage. The viable egg and larval production per spawn per kg female were significantly improved by the broodstock selected for high growth trait and fed with fish oil-based diet. This present study results signifies that gilthead seabream broodstock selected on growth trait could have positive role in improvement of sperm and egg quality to produce viable progeny.

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