scholarly journals Sugar Starvation Enhances Leaf Senescence and Genes Involved in Sugar Signaling Pathways Regulate Early Leaf Senescence in Mutant Rice

Rice Science ◽  
2020 ◽  
Vol 27 (3) ◽  
pp. 201-214
Author(s):  
Li Zhaowei ◽  
Zhao Qian ◽  
Cheng Fangmin
Keyword(s):  
Signaling Pathways ◽  
Leaf Senescence ◽  
Sugar Signaling ◽  
Sugar Starvation ◽  
Early Leaf Senescence

scholarly journals Physiological and Transcriptome Analyses of Early Leaf Senescence for ospls1 Mutant Rice (Oryza sativa L.) during the Grain-Filling Stage

2019 ◽  
Vol 20 (5) ◽  
pp. 1098 ◽  
Author(s):  
Zhaowei Li ◽  
Xinfeng Pan ◽  
Xiaodong Guo ◽  
Kai Fan ◽  
Wenxiong Lin
Keyword(s):  
Oryza Sativa ◽  
Signaling Pathways ◽  
Leaf Senescence ◽  
Grain Filling ◽  
Differentially Expressed ◽  
Hormone Signaling ◽  
Rna Seq ◽  
Filling Stage ◽  
Grain Filling Stage ◽  
Early Leaf Senescence

Early leaf senescence is an important agronomic trait that affects crop yield and quality. To understand the molecular mechanism of early leaf senescence, Oryza sativa premature leaf senescence 1 (ospls1) mutant rice with a deletion of OsVHA-A and its wild type were employed in this study. The genotype-dependent differences in photosynthetic indexes, senescence-related physiological parameters, and yield characters were investigated during the grain-filling stage. Moreover, RNA sequencing (RNA-seq) was performed to determine the genotype differences in transcriptome during the grain-filling stage. Results showed that the ospls1 mutant underwent significant decreases in the maximal quantum yield of photosystem II (PSII) photochemistry (Fv/Fm), net photosynthesis rate (Pn), and soluble sugar and protein, followed by the decreases in OsVHA-A transcript and vacuolar H+-ATPase activity. Finally, yield traits were severely suppressed in the ospls1 mutant. RNA-seq results showed that 4827 differentially expressed genes (DEGs) were identified in ospls1 mutant between 0 day and 14 days, and the pathways of biosynthesis of secondary metabolites, carbon fixation in photosynthetic organisms, and photosynthesis were downregulated in the senescing leaves of ospls1 mutant during the grain-filling stage. In addition, 81 differentially expressed TFs were identified to be involved in leaf senescence. Eleven DEGs related to hormone signaling pathways were significantly enriched in auxin, cytokinins, brassinosteroids, and abscisic acid pathways, indicating that hormone signaling pathways participated in leaf senescence. Some antioxidative and carbohydrate metabolism-related genes were detected to be differentially expressed in the senescing leaves of ospls1 mutant, suggesting that these genes probably play response and regulatory roles in leaf senescence.

scholarly journals Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

2014 ◽  
Vol 165 (4) ◽  
pp. 1505-1520 ◽  
Author(s):  
Dmitry Yarmolinsky ◽  
Galina Brychkova ◽  
Assylay Kurmanbayeva ◽  
Aizat Bekturova ◽  
Yvonne Ventura ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Sulfite Reductase ◽  
Tomato Plants ◽  
Early Leaf Senescence

scholarly journals Arabidopsis mlo3 mutant plants exhibit spontaneous callose deposition and signs of early leaf senescence

2019 ◽  
Vol 101 (1-2) ◽  
pp. 21-40 ◽  
Author(s):  
Stefan Kusch ◽  
Susanne Thiery ◽  
Anja Reinstädler ◽  
Katrin Gruner ◽  
Krzysztof Zienkiewicz ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Callose Deposition ◽  
Early Leaf Senescence

scholarly journals High-Resolution Mapping of the Novel Early Leaf Senescence Gene Els2 in Common Wheat

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 698
Author(s):  
Na Wang ◽  
Yanzhou Xie ◽  
Yingzhuang Li ◽  
Shengnan Wu ◽  
Shuxian Li ◽  
...  
Keyword(s):  
High Resolution ◽  
Leaf Senescence ◽  
Chinese Spring ◽  
Snp Array ◽  
Triticum Aestivum L ◽  
Snp Markers ◽  
F2 Population ◽  
High Resolution Map ◽  
Kasp Markers ◽  
Early Leaf Senescence

Early leaf senescence negatively impacts the grain yield in wheat (Triticum aestivum L.). Induced mutants provide an important resource for mapping and cloning of genes for early leaf senescence. In our previous study, Els2, a single incomplete dominance gene, that caused early leaf senescence phenotype in the wheat mutant LF2099, had been mapped on the long arm of chromosome 2B. The objective of this study was to develop molecular markers tightly linked to the Els2 gene and construct a high-resolution map surrounding the Els2 gene. Three tightly linked single-nucleotide polymorphism (SNP) markers were obtained from the Illumina Wheat 90K iSelect SNP genotyping array and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. To saturate the Els2 region, the Axiom® Wheat 660K SNP array was used to screen bulked extreme phenotype DNA pools, and 9 KASP markers were developed. For fine mapping of the Els2 gene, these KASP markers and previously identified polymorphic markers were analyzed in a large F2 population of the LF2099 × Chinese Spring cross. The Els2 gene was located in a 0.24-cM genetic region flanked by the KASP markers AX-111643885 and AX-111128667, which corresponded to a physical interval of 1.61 Mb in the Chinese Spring chromosome 2BL containing 27 predicted genes with high confidence. The study laid a foundation for a map-based clone of the Els2 gene controlling the mutation phenotype and revealing the molecular regulatory mechanism of wheat leaf senescence.

Comparative proteomic analysis of the maize responses to early leaf senescence induced by preventing pollination

2018 ◽  
Vol 177 ◽  
pp. 75-87 ◽  
Author(s):  
Liancheng Wu ◽  
Shunxi Wang ◽  
Lei Tian ◽  
Liuji Wu ◽  
Mingna Li ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Early Leaf Senescence

scholarly journals Characterization of Somatic Embryogenesis Receptor-Like Kinase 4 as a Negative Regulator of Leaf Senescence in Arabidopsis

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 50 ◽  
Author(s):  
Xiaoxu Li ◽  
Salman Ahmad ◽  
Akhtar Ali ◽  
Cun Guo ◽  
Hong Li ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Leaf Senescence ◽  
Stress Responses ◽  
Negative Regulator ◽  
Knockout Mutant ◽  
Surface Receptors ◽  
Extracellular Signals ◽  
Early Leaf Senescence

Leaf senescence is a genetically controlled process that involves the perception of extracellular signals and signal transduction. The receptor-like protein kinases (RLKs) are known to act as an important class of cell surface receptors and are involved in multiple biological processes such as development and stress responses. The functions of a number of RLK members have been characterized in Arabidopsis and other plant species, but only a limited number of RLK proteins have been reported to be associated with leaf senescence. In the present study, we have characterized the role of the somatic embryogenesis receptor kinase 4 (SERK4) gene in leaf senescence. The expression of SERK4 was up-regulated during leaf senescence and by several abiotic stress treatments in Arabidopsis. The serk4-1 knockout mutant was found to display a significant early leaf senescence phenotype. Furthermore, the results of overexpression analysis and complementary analysis supported the idea that SERK4 acts as a negative regulator in the process of leaf senescence.

scholarly journals Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Liting Sun ◽  
Yihua Wang ◽  
Ling-long Liu ◽  
Chunming Wang ◽  
Ting Gan ◽  
...  
Keyword(s):  
Leaf Senescence ◽  
Defense Response ◽  
Isolation And Characterization ◽  
Early Leaf Senescence

scholarly journals Transcriptome Analysis Reveals Multiple Hormones, Wounding and Sugar Signaling Pathways Mediate Adventitious Root Formation in Apple Rootstock

2018 ◽  
Vol 19 (8) ◽  
pp. 2201 ◽  
Author(s):  
Ke Li ◽  
Yongqi Liang ◽  
Libo Xing ◽  
Jiangping Mao ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Signalling Pathways ◽  
Auxin Signaling ◽  
Strong Positive Correlation ◽  
Sugar Signaling ◽  
Apple Rootstocks ◽  
Auxin Signaling Pathway

Adventitious roots (AR) play an important role in the vegetative propagation of apple rootstocks. The potential role of hormone, wounding, and sugar signalling pathways in mediating AR formation has not been adequately explored and the whole co-expression network in AR formation has not been well established in apple. In order to identify the molecular mechanisms underlying AR formation in ‘T337’ apple rootstocks, transcriptomic changes that occur during four stages of AR formation (0, 3, 9 and 16 days) were analyzed using high-throughput sequencing. A total of 4294 differentially expressed genes were identified. Approximately 446 genes related to hormones, wounding, sugar signaling, root development, and cell cycle induction pathways were subsequently selected based on their potential to be involved in AR formation. RT-qPCR validation of 47 genes with known functions exhibited a strong positive correlation with the RNA-seq data. Interestingly, most of the candidate genes involved in AR formation that were identified by transcriptomic sequencing showed auxin-responsive expression patterns in an exogenous Indole-3-butyric acid (IBA)-treatment assay: Indicating that endogenous and exogenous auxin plays key roles in regulating AR formation via similar signalling pathways to some extent. In general, AR formation in apple rootstocks is a complex biological process which is mainly influenced by the auxin signaling pathway. In addition, multiple hormones-, wounding- and sugar-signaling pathways interact with the auxin signaling pathway and mediate AR formation in apple rootstocks.

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