Biogenic robust synthesis of silver nanoparticles using Punica granatum peel and its application as a green catalyst for the reduction of an anthropogenic pollutant 4-nitrophenol

Author(s):  
T. Jebakumar Immanuel Edison ◽  
M.G. Sethuraman
2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.


2018 ◽  
Vol 13 (1) ◽  
Author(s):  
Sandhanasamy Devanesan ◽  
Mohamad S AlSalhi ◽  
Radhakrishnan Vishnu Balaji ◽  
Amirtham Jacob A Ranjitsingh ◽  
Anis Ahamed ◽  
...  

2020 ◽  
pp. 1-10 ◽  
Author(s):  
K. Muthu ◽  
S. Rajeswari ◽  
B. Akilandaeaswari ◽  
S. M. Nagasundari ◽  
R. Rangasamy

2020 ◽  
Vol 1660 ◽  
pp. 012013
Author(s):  
Maallah T. Al-Husseini ◽  
Harith R. Al-Mousawi ◽  
Naser Jawad Kadhim ◽  
Ahmed Abd Al-Redaa Madhloom ◽  
Dhifaf Zeki Aziz ◽  
...  

2020 ◽  
Vol 10 (6) ◽  
pp. 868-875
Author(s):  
Lokesh Ravi ◽  
Riven Chocalingam ◽  
Vignesh Menta ◽  
Kannabiran Krishnan

Background: Antimicrobial potential of silver nanoparticles synthesised by using various biological sources was already been reported by many researchers. The green synthesis of silver nanoparticles using microbial sources has been proved to be more effective. Methods: In this study, anti-dermatophytic silver nanoparticles were synthesised by using pyrrolo metabolites producing actinomycetes as a green catalyst. Different characterization methods such as UV-Visible, XRD, and AFM were used to identify the physiochemical characteristics of synthesised nanoparticles. Results: The synthesised nanoparticles showed λ-max at 427 nm and 402 nm, respectively. The XRD analysis based on the JCPDS database identified the two synthesized nanoparticles as silver oxide nanoparticles (AgO NPs) and silver peroxide nanoparticles (Ag2O3 NPs). The size of these nanoparticles was found to be in the range of 40-44 nm (AgO NPs) and 23-25 nm (Ag2O3 NPs), respectively. The synthesized nanoparticles demonstrated significant anti-fungal activity against dermatophytic fungi Trichophyton mentagrophyes with the zone of inhibition of 38 mm by AgO NP and 17 mm by Ag2O3 NPs. Conclusion: Screening of marine actinomycetes LG003 and LG005 revealed the presence of pyrrolo derivatives as the major metabolites, suggesting that these pyrrolo derivatives could be responsible for synthesis and stabilization of AgO and Ag2O3NPs. Among the synthesized NPs, the AgO NPs showed great potential as an anti-dermatophytic agent. This study provides further research opportunities for AgONPs as anti-fungal agents.


2016 ◽  
Vol 10 (4) ◽  
pp. 282-287 ◽  
Author(s):  
Shakeel Ahmed ◽  
. Annu ◽  
Kaiser Manzoor ◽  
Saiqa Ikram

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5762
Author(s):  
Monisha Singhal ◽  
Sreemoyee Chatterjee ◽  
Ajeet Kumar ◽  
Asad Syed ◽  
Ali H. Bahkali ◽  
...  

The current research work illustrates an economical and rapid approach towards the biogenic synthesis of silver nanoparticles using aqueous Punica granatum leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters), namely concentration of AgNO3, temperature and ratio of extract to AgNO3, on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated, which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of the functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial and antibiofilm properties.


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