Fungal endophytes from medicinal plant Bletilla striata (Thunb.) Reichb. F. promote the host plant growth and phenolic accumulation

2021 ◽  
Vol 143 ◽  
pp. 25-32
Author(s):  
Jianhua Chen ◽  
Lili Li ◽  
Peiwen Tian ◽  
Wei Xiang ◽  
Xuan Lu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Medicinal Plant ◽  
Host Plant ◽  
Fungal Endophytes ◽  
Bletilla Striata

scholarly journals Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

2021 ◽  
Vol 7 (10) ◽  
pp. 788
Author(s):  
Chuan You ◽  
Dan Qin ◽  
Yumeng Wang ◽  
Wenyi Lan ◽  
Yehong Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Host Plant ◽  
Functional Screening ◽  
Schisandra Sphenanthera ◽  
Metabolite Accumulation ◽  
Bacteria And Fungi

Beneficial interactions between endophytes and plants are critical for plant growth and metabolite accumulation. Nevertheless, the secondary metabolites controlling the feedback between the host plant and the endophytic microbial community remain elusive in medicinal plants. In this report, we demonstrate that plant-derived triterpenoids predominantly promote the growth of endophytic bacteria and fungi, which in turn promote host plant growth and secondary metabolite productions. From culturable bacterial and fungal microbial strains isolated from the medicinal plant Schisandra sphenanthera, through triterpenoid-mediated screens, we constructed six synthetic communities (SynComs). By using a binary interaction method in plates, we revealed that triterpenoid-promoted bacterial and fungal strains (TPB and TPF) played more positive roles in the microbial community. The functional screening of representative strains suggested that TPB and TPF provide more beneficial abilities to the host. Moreover, pot experiments in a sterilized system further demonstrated that TPB and TPF play important roles in host growth and metabolite accumulation. In summary, these experiments revealed a role of triterpenoids in endophytic microbiome assembly and indicated a strategy for constructing SynComs on the basis of the screening of secondary metabolites, in which bacteria and fungi join forces to promote plant health. These findings may open new avenues towards the breeding of high yielding and high metabolite-accumulating medicinal plants by exploiting their interaction with beneficial endophytes.

scholarly journals Endophytic Colonisation of Solanum lycopersicum and Phaseolus vulgaris by Fungal Endophytes Promotes Seedlings Growth and Hampers the Reproductive Traits, Development, and Survival of the Greenhouse Whitefly, Trialeurodes vaporariorum

2021 ◽  
Vol 12 ◽  
Author(s):  
Vongai M. Paradza ◽  
Fathiya M. Khamis ◽  
Abdullahi A. Yusuf ◽  
Sevgan Subramanian ◽  
Sunday Ekesi ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Host Plant ◽  
Solanum Lycopersicum ◽  
Host Plants ◽  
Fungal Endophytes ◽  
Trialeurodes Vaporariorum ◽  
Greenhouse Whitefly ◽  
Plant Parts ◽  
Fungal Isolates

In the scope of mitigating the negative impacts of pesticide use and managing greenhouse whiteflies, Trialeurodes vaporariorum sustainably, 16 endophytic fungal isolates from five different genera (Beauveria, Trichoderma, Hypocrea, Bionectria, and Fusarium) were screened for their ability to colonise two preferred host plant species, namely, tomato (Solanum lycopersicum L.) and French bean (Phaseolus vulgaris L.), through seed inoculation. Seven and nine isolates were endophytic to P. vulgaris and S. lycopersicum, respectively, where significant differences in the endophytic colonisation rates were observed among the fungal isolates in P. vulgaris and its plant parts, with a significant interaction between the isolates and plant parts in S. lycopersicum. Hypocrea lixii F3ST1, Trichoderma asperellum M2RT4, Trichoderma atroviride F5S21, and T. harzianum KF2R41 successfully colonised all the plant parts of both hosts and therefore were selected and further evaluated for their endophytic persistence, effect on plant growth, and pathogenicity to T. vaporariorum adults and F1 progeny. The four endophytes remained in both host plants for the 5-week assessment with varied colonisation rates related to the strong interaction with the time, isolates, and plant parts in both hosts. The effect of the same endophytes on the different host growth parameters varied in P. vulgaris and S. lycopersicum, with T. asperellum M2RT4 not boosting the growth in both host plants while T. atroviride F5S21 resulted in enhanced shoot biomass in S. lycopersicum. T. atroviride F5S21 and T. harzianum KF2R41 inoculated S. lycopersicum plants and H. lixii F3ST1, T. asperellum M2RT4, and T. harzianum KF2R41 inoculated P. vulgaris plants had significantly lower oviposition, while nymph development in both hosts was significantly prolonged in all the endophytically–colonised plants. The endophytes H. lixii F3ST1 and T. asperellum M2RT4 significantly reduced the longevity/survival of the exposed T. vaporariorum adults and the progeny in both S. lycopersicum and P. vulgaris. The findings demonstrate the attributes of the various endophytes in host plant growth promotion as well as their effects on the life-history parameters of T. vaporariorum and could consequently be developed as potential endophytic fungal-based biopesticides for the sustainable management of the pest in S. lycopersicum and P. vulgaris cropping systems.

scholarly journals Isolation and Characterization of Fungal Endophytes Isolated from Medicinal Plant Ephedra pachyclada as Plant Growth-Promoting

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 140 ◽  
Author(s):  
Ahmed Mohamed Aly Khalil ◽  
Saad El-Din Hassan ◽  
Sultan M. Alsharif ◽  
Ahmed M. Eid ◽  
Emad El-Din Ewais ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Growth ◽  
Aspergillus Flavus ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Penicillium Crustosum ◽  
Plant Growth Promoting ◽  
Penicillium Commune ◽  
Maize Plants ◽  
Growth Promoting

Endophytic fungi are widely present in internal plant tissues and provide different benefits to their host. Medicinal plants have unexplored diversity of functional fungal association; therefore, this study aimed to isolate endophytic fungi associated with leaves of medicinal plants Ephedra pachyclada and evaluate their plant growth-promoting properties. Fifteen isolated fungal endophytes belonging to Ascomycota, with three different genera, Penicillium, Alternaria, and Aspergillus, were obtained from healthy leaves of E. pachyclada. These fungal endophytes have varied antimicrobial activity against human pathogenic microbes and produce ammonia and indole acetic acid (IAA), in addition to their enzymatic activity. The results showed that Penicillium commune EP-5 had a maximum IAA productivity of 192.1 ± 4.04 µg mL−1 in the presence of 5 µg mL−1 tryptophan. The fungal isolates of Penicillium crustosum EP-2, Penicillium chrysogenum EP-3, and Aspergillus flavus EP-14 exhibited variable efficiency for solubilizing phosphate salts. Five representative fungal endophytes of Penicillium crustosum EP-2, Penicillium commune EP-5, Penicillium caseifulvum EP-11, Alternaria tenuissima EP-13, and Aspergillus flavus EP-14 and their consortium were selected and applied as bioinoculant to maize plants. The results showed that Penicillium commune EP-5 increased root lengths from 15.8 ± 0.8 to 22.1 ± 0.6. Moreover, the vegetative growth features of inoculated maize plants improved more than the uninoculated ones.

scholarly journals Analysis of plant growth-promoting properties of Bacillus amyloliquefaciens UCMB5113 using Arabidopsis thaliana as host plant

Planta ◽  
2016 ◽  
Vol 245 (1) ◽  
pp. 15-30 ◽  
Author(s):  
Shashidar Asari ◽  
Danuše Tarkowská ◽  
Jakub Rolčík ◽  
Ondřej Novák ◽  
David Velázquez Palmero ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Host Plant ◽  
Bacillus Amyloliquefaciens ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Biomass Production of Root and Shoot of Talinum paniculatum Gaertn. by Liquid and Solid Ms Medium with Plant Growth Hormone IBA

2017 ◽  
Vol 1 (2) ◽  
pp. 85
Author(s):  
Muhammad Hamzah Solim ◽  
Y. S. Wulan Manuhara
Keyword(s):  
Growth Hormone ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Biomass Production ◽  
Blood Circulation ◽  
Raw Material ◽  
Ms Medium ◽  
Shoot Morphology ◽  
Dry Biomass

Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia which has benefits such as for vitality and maintain blood circulation. The aim of this research is to obtain biomass production of root and shoot of T. paniculatum Gaertn. by liquid and solid MS medium with IBA. This research conducted to provide biomass as raw material for secondary metabolites test. Stems as explant were induced with four treatments (liquid MS, solid MS, liquid MS + 2 ppm IBA and solid MS + 2 ppm IBA) with five repetitions. Observation did for 28 days. The parameters are the percentage of explants which formed the root and shoot, morphology, fresh and dry biomass. Result shows that percentage of root and shoot have 100% in liquid and solid MS + 2 ppm IBA. Fresh and dry biomass of root and shoot in solid MS + 2 ppm IBA higher than the others. This research found callus in liquid and solid MS + 2 ppm IBA. Morphology of root in liquid MS has thin and friable, but thick in solid MS. Shoot in solid and liquid MS has thin, short and sturdy.

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