At hand, be a lot of process to production of leather, such as soaking, liming, degreasing, pickling and tanning process. This makes huge quantity of waste water manufacture from tannery industry. Tannery effluents are additional cause to the environment. An Indian tannery industry generates the tannery effluent regarding 50,000 m3/ day. As a result, treating tannery effluent is the majority chief assignment to save the adjacent area and to get better fresh water. In this exploration the sample from the tanning industry waste water undergo Biological behavior development, typically tanning industry waste water is an effectual within the organichealingprogression. In this learningalongsideby means of the waste matter, the activated slush from Sewage Treatment Plant is additional as a seeding material in the proportion of 95%, 90%, 80%, 70%, 60%, 50%, replacein the 500ml serum bottle in addition to closed tightly to keep up Anaerobic situation and evaluate their biogas production day by day. The 60% activated sludge and the 40% tannery waste waters Mixture gave the high yield of biogas and degraded the macrobiotic contaminants efficiently. From this study, it is observed that activated Sludge is use as seeding materials to biodegrade the natural pollutant at hand in the tannery waste water.