Competitive use of root exudates by Bacillus amyloliquefaciens with Ralstonia solanacearum decreases the pathogenic population density and effectively controls tomato bacterial wilt

2017 ◽  
Vol 218 ◽  
pp. 132-138 ◽  
Author(s):  
Kai Wu ◽  
Lv Su ◽  
Zhiying Fang ◽  
Saifei Yuan ◽  
Lili Wang ◽  
...  
Keyword(s):  
Population Density ◽  
Root Exudates ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacillus Amyloliquefaciens ◽  
Tomato Bacterial Wilt

Potassium phosphite enhances the antagonistic capability of Bacillus amyloliquefaciens to manage tomato bacterial wilt

Plant Disease ◽  
2021 ◽  
Author(s):  
Lv Su ◽  
Pengfei Qiu ◽  
Zhiying Fang ◽  
Xingxia Mo ◽  
Juan Sun ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacillus Amyloliquefaciens ◽  
Combined Treatment ◽  
Wilt Disease ◽  
Agricultural Chemicals ◽  
Bacterial Wilt Disease ◽  
Potassium Phosphite ◽  
Novel Strategy ◽  
Tomato Bacterial Wilt

Bacterial wilt caused by Ralstonia solanacearum is a distributed and worldwide soil-borne disease. The application of biocontrol microbes or agricultural chemicals has been widely used to manage tomato bacterial wilt. However, whether and how agricultural chemicals affect the antagonistic ability of biocontrol microbes is still unknown. Here, we combined potassium phosphite (K-Phite), an environmentally friendly agricultural chemical, and the biocontrol agent Bacillus amyloliquefaciens QPF8 (strain F8) to manage tomato bacterial wilt disease. First, K-Phite at a concentration of 0.05% (w/v) could significantly inhibit the growth of Ralstonia solanacearum. Second, 0.05% K-Phite enhanced the antagonistic capability of B. amyloliquefaciens F8. Third, the greenhouse soil experiments showed that the control efficiency for tomato bacterial wilt in the combined treatment was significantly higher than that of the application of B. amyloliquefaciens F8 or K-Phite alone. Overall, our results highlighted a novel strategy for the control of tomato bacterial wilt disease via application and revealed a new integrated pattern depending on the enhancement of the antagonistic capability of biocontrol microbes by K-Phite.

Isolation of Bacillus amyloliquefaciens JK6 and identification of its lipopeptides surfactin for suppressing tomato bacterial wilt

RSC Advances ◽  
2015 ◽  
Vol 5 (100) ◽  
pp. 82042-82049 ◽  
Author(s):  
Hanqin Xiong ◽  
Yongtao Li ◽  
Yanfei Cai ◽  
Yu Cao ◽  
Yan Wang
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacillus Amyloliquefaciens ◽  
Rhizosphere Soil ◽  
Tomato Plants ◽  
Tomato Bacterial Wilt

A rhizobacteria strain,B.amyloliquefaciensJK6, isolated from the rhizosphere soil of healthy tomato plants, significantly inhibitedRalstonia solanacearum(RS).

Effectiveness of Egyptian propolis on control of tomato bacterial wilt caused by Ralstonia solanacearum

2017 ◽  
Vol 124 (5) ◽  
pp. 467-472 ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Mohamed E. A. Seleim ◽  
Rafeek M. El-Sharkawy ◽  
Hadel M. M. Khalil Bagy
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Tomato Bacterial Wilt

scholarly journals Evaluation of biological efficacy of Trichoderma asperellum against tomato bacterial wilt caused by Ralstonia solanacearum

2018 ◽  
Vol 28 (1) ◽  
Author(s):  
Narasimhamurthy Konappa ◽  
Soumya Krishnamurthy ◽  
Chandra Nayaka Siddaiah ◽  
Niranjana Siddapura Ramachandrappa ◽  
Srinivas Chowdappa
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Trichoderma Asperellum ◽  
Biological Efficacy ◽  
Tomato Bacterial Wilt

Distribution and incidence of tomato bacterial wilt caused by Ralstonia solanacearum in the central region of Togo

2021 ◽  
Author(s):  
Bitang Bamazi ◽  
Agnassim Banito ◽  
K. D. Ayisah ◽  
Rachidatou Sikirou ◽  
Mathews Paret ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Laboratory Investigation ◽  
Central Region ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Yield Losses ◽  
Tomato Production ◽  
Solanum Lycopersicum L ◽  
Survey Results ◽  
Tomato Bacterial Wilt

Tomato (Solanum lycopersicum L.) is one of the most important vegetables in Togo. Unfortunately, tomatoes are susceptible to many diseases, among which bacterial wilt caused by Ralstonia solanacearum causes major yield losses. In this study, incidence of bacterial wilt and its distribution was evaluated in the central region of Togo, the major tomato producing area in the country. Overall, 16 localities were surveyed in four prefectures. In each locality, three fields were visited, and the incidence of the disease was recorded, and diseased samples were collected for laboratory investigation. The results showed that bacterial wilt occurred in all the fields visited, indicating a field incidence of 100%, whereas the plant incidence ranged from 10.00±00% to 43.33±3.33%, with an average of 20.94±1.77%. The antibody based Immunostrip test was positive for R. solanacearum in 100% of the visited fields. From 144 samples collected from fields, 45 R. solanacearum isolates were isolated on Modified SMSA media. This survey results show that tomato bacterial wilt is a real threat to tomato production in the central region of Togo.

scholarly journals Bacillus amyloliquefaciens Strain PMB05 Intensifies Plant Immune Responses to Confer Resistance Against Bacterial Wilt of Tomato

2020 ◽  
Vol 110 (12) ◽  
pp. 1877-1885
Author(s):  
Ting-Hsin Ho ◽  
Chiao-Yu Chuang ◽  
Jing-Lin Zheng ◽  
Hong-Hua Chen ◽  
Yu-Shen Liang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Bacterial Wilt ◽  
Bacillus Amyloliquefaciens ◽  
Plant Immunity ◽  
Reactive Oxygen Species Generation ◽  
Soft Rot ◽  
Limiting Factors ◽  
Callose Deposition ◽  
Bacillus Spp ◽  
Tomato Bacterial Wilt

Tomato is an economic crop worldwide. Many limiting factors reduce the production of tomato, with bacterial wilt caused by Ralstonia solanacearum being the most destructive disease. Our previous study showed that the disease resistance to bacterial soft rot is enhanced by Bacillus amyloliquefaciens strain PMB05. This enhanced resistance is associated with the intensification of pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI). To determine whether the PTI-intensifying Bacillus spp. strains are able to confer disease resistance to bacterial wilt, their effects on PTI signals triggered by PAMP from R. solanacearum and on the occurrence of bacterial wilt were assayed. Before assay, a gene that encodes harpin from R. solanacearum, PopW, was applied as a PAMP. Results revealed that the B. amyloliquefaciens strain PMB05 was the one strain among 9 Bacillus rhizobacterial strains which could significantly intensify the PopW-induced hypersensitive response (HR) on Arabidopsis leaves. Moreover, we observed that the signals of PopW-induced reactive oxygen species generation and callose deposition were increased, confirming that the PTI was intensified by PMB05. The intensification of the PopW-triggered HR by PMB05 in Arabidopsis was reduced upon treatment with inhibitors in PTI pathways. Furthermore, the application of Bacillus spp. strains on tomato plants showed that only the use of PMB05 resulted in significantly increased resistance to bacterial wilt. Moreover, the PTI signals were also intensified in the tomato leaves. Taken together, we demonstrated that PMB05 is a PTI-intensifying bacterium that confers resistance to tomato bacterial wilt. Screening of plant immunity intensifying rhizobacteria is a possible strategy to control tomato bacterial wilt. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Effect of Allium fistulosum Extract on Ralstonia solanacearum Populations and Tomato Bacterial Wilt

Plant Disease ◽  
2012 ◽  
Vol 96 (5) ◽  
pp. 687-692 ◽  
Author(s):  
Péninna Deberdt ◽  
Benjamin Perrin ◽  
Régine Coranson-Beaudu ◽  
Pierre-François Duyck ◽  
Emmanuel Wicker
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Management Strategies ◽  
Antimicrobial Effect ◽  
Allium Fistulosum ◽  
Inhibition Assay ◽  
In Vivo Experiments ◽  
Tomato Bacterial Wilt

To control bacterial wilt (Ralstonia solanacearum, phylotype IIB/4NPB), the antimicrobial effect of Allium fistulosum aqueous extract was assessed as a preplant soil treatment. Three concentrations of extract (100, 50, and 25%, 1:1 [wt/vol]) were evaluated by in vitro inhibition assay and in vivo experiments in a growth chamber. In vitro, A. fistulosum (100 and 50%) suppressed growth of R. solanacearum. Preplant treatment of the soil with A. fistulosum extract significantly reduced the R. solanacearum populations. No pathogen was detected in the soil after treatment with 100% concentrated extract from the third day after application until the end of the experiment. A. fistulosum also significantly reduced the incidence of tomato bacterial wilt. In the untreated control, the disease affected 61% of the plants whereas, with 100 and 50% extracts, only 6 and 14% of the plants, respectively, were affected. These results suggest that A. fistulosum extracts could be used in biocontrol-based management strategies for bacterial wilt of tomato.

Sign in / Sign up

Export Citation Format

Share Document