Investigating the enzymatic and non-enzymatic antioxidant defense by applying iron oxide nanoparticles in Dracocephalum moldavica L. plant under salinity stress

2020 ◽  
Vol 272 ◽  
pp. 109537 ◽  
Author(s):  
Hanieh Moradbeygi ◽  
Rashid Jamei ◽  
Reza Heidari ◽  
Reza Darvishzadeh
Biologija ◽  
2017 ◽  
Vol 63 (1) ◽  
Author(s):  
Meheri Askary ◽  
Seyed Mehdi Talebi ◽  
Fariba Amini ◽  
Ali Dousti Balout Bangan

The progress of nanotechnology presents many nanoparticles that are important in medicine, agriculture and industry. Quickly and entirely absorbed by plants, nano-compounds and remedy their nutrient deficiency and satisfy this need. Iron oxide nanoparticles with suitable surface chemistry can be used as a rich source of iron for plants due to its gradual release of Fe in a wide pH range (pH 3 to 11). The present study investigated the impact of iron oxide nanoparticles (Fe2O3NPs in 0, 10, 20 and 30  µM concentrations) on physiological parameters of peppermint (Mentha piperita) under salt stress (0, 50, 100 and 150 mM concentrations of NaCl). Fe2O3NPs caused increases in leaf fresh weight and dry weight, phosphorus, potassium, iron, zinc, and calcium contents of the peppermint under salinity stress but did not have an effect on the sodium element. 30 µM concentration of Fe2O3NP was more impressive. Lipid peroxidation and proline contents of the peppermint under salinity decreased significantly by applying Fe2O3NPs. The maximum activities of total antioxidant enzymes (I %), catalase, superoxide dismutase, and guailcol peroxidase were observed in plants treated with 150 mM of NaCl, but application of Fe2O3NPs declined these antioxidant activities. The results suggest that the appropriate concentration of iron nanoparticles could be used for stress resistance of the peppermint.


Author(s):  
Davinder Singh ◽  
Devendra Sillu ◽  
Anil Kumar ◽  
Shekhar Agnihotri

Nanomaterials possessing enzyme like activities have shown immense potential to improve plants’ performance under unfavorable conditions such as abiotic stresses. Although several strategies for evaluating nano-bio interactions have been evidenced...


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.


2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>


2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>


2020 ◽  
Vol 2020 (3) ◽  
pp. 54-61
Author(s):  
S.E. Litvin ◽  
◽  
Yu.A. Kurapov ◽  
E.M. Vazhnichaya ◽  
Ya.A. Stel’makh ◽  
...  

2015 ◽  
Vol 22 (15) ◽  
pp. 1808-1828 ◽  
Author(s):  
Diana Couto ◽  
Marisa Freitas ◽  
Felix Carvalho ◽  
Eduarda Fernandes

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