Foliar application of pig blood-derived protein hydrolysates improves antioxidant activities in lettuce by regulating phenolic biosynthesis without compromising yield production

Lettuce (Lactuca sativa L.) is a leafy vegetable cultivated widely for its fast and year-round production and its beneficial phytochemical content, which may be boosted further by plant biostimulants that are considered eco-sustainable means for enhancing horticultural crop production. A greenhouse experiment was carried out to evaluate the yield and qualitative parameters of two differently pigmented lettuce cultivars grown in a floating raft system either untreated or treated (leaf, root or leaf/root application) with vegetal protein hydrolysates (PHs). For foliar application (F), lettuce plants were sprayed at a dose of 3 mL L−1, whereas for root application, 0.15 (T1) or 0.3 (T2) mL L−1 was applied to the nutrient solution alone or in combination with foliar spray (T1 + F and T2 + F) with the same foliar concentration. Bio-morphometric and production data were collected after harvest. Physiological and plant nutrition assays included leaf gas exchange, leaf fluorescence, SPAD index, mineral content, carotenoids, total phenols, total ascorbic acid content and antioxidant activities. Cultivar-specific reactions to biostimulant application were noted: whilst the green pigmented cultivar thrived under nutrient solution applications and recorded higher yield by 82.7% (T1) or (T1 + F) and 71.7% (T2), the red cultivar thrived under combined treatments, yielding 55.4% (T2 + F) higher than control and providing the most concentrated phytochemical content. These latter treatments also engendered the highest SPAD index, Fv/Fm ratio, CO2 assimilation, stomatal conductance and transpiration. In addition, the T2 + F treatment boosted ‘Canasta’ hydrophilic antioxidant activity (21.9%) and total ascorbic acid (5.6-fold). Nutrient solution treatments alone proved advantageous when compared to foliar treatments, while mixed treatments proved genotype-specific. New research on genotype specificity of biostimulant effects is warranted for future use, in order to rationalize biostimulant application modes and dosages.

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Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice

Nutrients ◽

10.3390/nu13072137 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2137

Author(s):

Jessica Maria Abbate ◽

Francesco Macrì ◽

Francesca Arfuso ◽

Carmelo Iaria ◽

Fabiano Capparucci ◽

...

Keyword(s):

Human Health ◽

Antioxidant Activities ◽

Protein Hydrolysate ◽

Protein Hydrolysates ◽

Metabolic Effects ◽

Lipid Lowering ◽

High Frequency Ultrasound ◽

Aortic Sinus ◽

Fish Waste ◽

Plaque Development

Fish protein consumption exerts beneficial metabolic effects on human health, also correlating with a decreased risk for cardiovascular disease. Fish waste contains high amount of proteins and utilization may offer the opportunity for generating compounds advantageous for human health. Especially, fish waste protein hydrolysates beneficially influence pathways involved in body composition, exerting anti-inflammatory and antioxidant activities, making their potential supplementation in human disorders of increased interest. This study assessed the effect of a 10% (w/w) anchovy waste protein hydrolysate (APH) diet for 12 weeks in reducing atherosclerosis in ApoE−/− mice, through histological and immunohistochemical methods. In addition, monitoring of plaque development was performed, using high-frequency ultrasound and magnetic resonance imaging. Overall, the APH diet attenuated atherosclerotic plaque development, producing a regression of arterial lesions over time (p < 0.05). Twelve weeks on an APH diet had an anti-obesity effect, improving lipid metabolism and reducing hepatic enzyme activity. A significant reduction in plaque size and lipid content was observed in the aortic sinus of APH-fed mice, compared to the control (p < 0.001), whereas no differences in the extracellular matrix and macrophage recruitment were observed. Supplementation of APH significantly attenuates atherosclerosis in ApoE−/− mice, exerting a lipid-lowering activity. The opportunity to use fish waste protein hydrolysates as a nutraceutical in atherosclerosis is worthy of future investigations, representing a low cost, sustainable, and nutritional strategy with minimal environmental impact.

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Fractionation of Protein Hydrolysates of Fish Waste Using Membrane Ultrafiltration: Investigation of Antibacterial and Antioxidant Activities

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-018-9483-y ◽

2018 ◽

Vol 11 (3) ◽

pp. 1015-1022 ◽

Cited By ~ 10

Author(s):

Samaneh Pezeshk ◽

Seyed Mahdi Ojagh ◽

Masoud Rezaei ◽

Bahareh Shabanpour

Keyword(s):

Antioxidant Activities ◽

Protein Hydrolysates ◽

Fish Waste

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Improvement of antioxidant activities and yield of spring maize through seed priming and foliar application of plant growth regulators under heat stress conditions

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n1p47 ◽

2017 ◽

Vol 38 (1) ◽

pp. 47 ◽

Cited By ~ 2

Author(s):

Ijaz Ahmad ◽

Shehzad Maqsood Ahmed Basra ◽

Muhammad Akram ◽

Allah Wasaya ◽

Muhammad Ansar ◽

...

Keyword(s):

Heat Stress ◽

Grain Yield ◽

Foliar Application ◽

Antioxidant Activities ◽

Foliar Spray ◽

Seed Priming ◽

Chlorophyll Contents ◽

Exogenous Application ◽

Hybrid Maize

Heat stress during reproductive and grain filling phases adversely affects the growth of cereals through reduction in grain’s number and size. However, exogenous application of antioxidants, plant growth regulators and osmoprotectants may be helpful to minimize these heat induced yield losses in cereals. This two year study was conducted to evaluate the role of exogenous application of ascorbic acid (AsA), salicylic acid (SA) and hydrogen peroxide (H2O2) applied through seed priming or foliar spray on biochemical, physiological, morphological and yield related traits, grain yield and quality of late spring sown hybrid maize. The experiment was conducted in the spring season of 2007 and 2008. We observed that application of AsA, SA and H2O2 applied through seed priming or foliar spray improved the physiological, biochemical, morphological and yield related traits, grain yield and grain quality of late spring sown maize in both years. In both years, we observed higher superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity in the plants where AsA, SA and H2O2were applied through seed priming or foliar spray than control. Membrane stability index (MSI), relative water contents (RWC), chlorophyll contents, grain yield and grain oil contents were also improved by exogenous application of AsA, SA and H2O2 in both years. Seed priming of AsA, SA and H2O2was equally effective as the foliar application. In conclusion, seed priming with AsA, SA and H2O2 may be opted to lessen the heat induced yield losses in late sown spring hybrid maize. Heat tolerance induced by ASA, SA and H2O2 may be attributed to increase in antioxidant activities and MSI which maintained RWC and chlorophyll contents in maize resulting in better grain yield in heat stress conditions.

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Enzymatic hydrolysis of chicken viscera proteins in the presence of an ionic liquid as a strategy to improve the antioxidant properties of the hydrolysates

Revista dos Trabalhos de Iniciação Científica da UNICAMP ◽

10.20396/revpibic2620181367 ◽

2019 ◽

Author(s):

Vitor Geniselli da Silva ◽

Ruann Janser Soares de Castro

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Enzymatic Hydrolysis ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Protein Hydrolysates ◽

Tetramethylammonium Bromide ◽

Chicken Viscera ◽

Hydrolysis Of

Aiming to explore the use of ionic liquids (ILs) not yet described in the literature, this work evaluated the hydrolysis of proteins from chicken viscera using the protease Alcalase modified and unmodified by the IL tetramethylammonium bromide. The protein hydrolysates produced in the presence of the IL presented values of antioxidant activities 40% higher than the hydrolysates obtained without IL. In addition, with the presence of the IL, it was possible to obtain protein hydrolysates from chicken viscera with similar antioxidant activities, compared to the protein hydrolysates produced without IL, using 1/3 of the amount of enzyme.

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Relief Role of Lysine Chelated Zinc (Zn) on 6-Week-Old Maize Plants under Tannery Wastewater Irrigation Stress

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17145161 ◽

2020 ◽

Vol 17 (14) ◽

pp. 5161

Author(s):

Rehan Ahmad ◽

Wajid Ishaque ◽

Mumtaz Khan ◽

Umair Ashraf ◽

Muhammad Atif Riaz ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Plant Growth ◽

Foliar Application ◽

Antioxidant Activities ◽

Foliar Spray ◽

Tannery Wastewater ◽

Organic Load ◽

Maize Plants

Tannery wastewater mainly comes from leather industries. It has high organic load, high salinity, and many other pollutants, including chromium (Cr). Tannery wastewater is generally used for crop irrigation in some areas of Pakistan and worldwide, due to the low availability of good quality of irrigation water. As tannery wastewater has many nutrients in it, its lower concentration benefits the plant growth, but at a higher concentration, it damages the plants. Chromium in tannery wastewater accumulates in plants, and causes stress at physiological and biochemical levels. In recent times, the role of micronutrient-amino acid chelated compounds has been found to be helpful in reducing abiotic stress in plants. In our present study, we used lysine chelated zinc (Zn-lys) as foliar application on maize (Zea mays L.), growing in different concentrations of tannery wastewater. Zinc (Zn) is required by plants for growth, and lysine is an essential amino acid. Maize plants were grown in tannery wastewater in four concentrations (0, 25%, 50%, and 100%) and Zn-lys was applied as a foliar spray in three concentrations (0 mM, 12.5 mM, and 25 mM) during plant growth. Plants were cautiously harvested right after 6 weeks of treatment. Foliar spray of Zn-lys on maize increased the biomass and improved the plant growth. Photosynthetic pigments such as total chlorophyll, chlorophyll a, chlorophyll b and contents of carotenoids also increased with Zn-lys application. In contrast to control plants, the hydrogen peroxide (H2O2) contents were increased up to 12%, 50%, and 68% in leaves, as well as 16%, 51% and 89% in roots at 25%, 50%, and 100% tannery water application, respectively, without Zn-lys treatments. Zn-lys significantly reduced the damages caused by oxidative stress in maize plant by decreasing the overproduction of H2O2 and malondialdehyde (MDA) in maize that were produced, due to the application of high amount of tannery wastewater alone. The total free amino acids and soluble protein decreased by 10%, 31% and 64% and 18%, 61% and 122% at 25%, 50% and 100% tannery water treatment. Zn-lys application increased the amino acids production and antioxidant activities in maize plants. Zn contents increased, and Cr contents decreased, in different parts of plants with Zn-lys application. Overall, a high concentration of tannery wastewater adversely affected the plant growth, but the supplementation of Zn-lys assertively affected the plant growth and enhanced the nutritional quality, by enhancing Zn and decreasing Cr levels in plants simultaneously irrigated with tannery wastewater.

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Antioxidant Properties of Microalgae Protein Hydrolysates Prepared by Neutral Protease Digestion

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.707.149 ◽

2014 ◽

Vol 707 ◽

pp. 149-153 ◽

Cited By ~ 2

Author(s):

Xiao Hu ◽

Xian Qing Yang ◽

Lai Hao Li ◽

Yan Yan Wu ◽

Wan Ling Lin ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Protein Hydrolysates ◽

Neutral Protease ◽

Degree Of Hydrolysis ◽

Protease Digestion ◽

Hydrolysis Of

Microalgae protein hydrolysates (MPH) were obtained by enzymatic hydrolysis of defatted microalgae meal using neutral protease. The protein recovery, degree of hydrolysis, and the antioxidant activities of the hydrolysates were investigated. The results demonstrated that hydrolysates prepared by neutral protease at 50 °C for 4 h exhibited the strongest antioxidant activity. Under these conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and the reducing power of the hydrolysates were 68.3%, 50.8% and 1.303, respectively.

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Prevention of Flower Abortion and Leaf Damages in Anemone coronaria by Ca Supplements

Israel Journal of Plant Sciences ◽

10.1163/22238980-00001038 ◽

2018 ◽

Vol 65 (1-2) ◽

pp. 97-108

Author(s):

Nirit Bernstein ◽

Mollie Sacks ◽

Piny Snir ◽

Rivka Rosenberg

Keyword(s):

Foliar Application ◽

Foliar Spray ◽

Leaf Tissue ◽

Yield Production ◽

Flower Abortion ◽

Flower Stem ◽

Irrigation Solution ◽

Calcium Supply ◽

Leaf Necrosis

Ca deficiencies induce a range of physiological disorders in plants. The disorders typically appear in young growing tissues that are characterized by high demand for Ca and restricted Ca supply due to low transpiration. In this study, we examined the effect of supplementing Ca by foliar spray and through the irrigation solution to Anemone coronaria plants, in order to evaluate if flower abortions and leaf damages that appear in the production fields are related to Ca deficiencies. With the goal to develop a preventive nutritional regime, four Ca treatments were evaluated. The supplemented Ca was applied with the fertigation solution in the concentrations of 60 or 110 ppm Ca; with the 60 ppm application an additional application of Ca by foliar application was tested in concentrations of 3 g/l Ca or 6 g/l Ca, as Ca(NO3)2. The plants were cultivated in a net-house, in soilless culture (Tuff) beds. Application of 110 ppm Ca compared to 60 ppm with the fertilizing solution increased the concentration of Ca in the leaf tissue, resulting in an increase in the quantity and quality of the flowers. Calcium supply by foliar spray, at both 3 g/l or 6 g/l Ca(NO3)2 caused leaf necrosis and did not improve yield production. Application of 110 ppm Ca reduced the concentrations of Mn, Cl and Na in the leaves. Application of Ca in the irrigation solution, or by foliar spray, did not reduce the percentage of non-marketable flowers. The identified lower concentrations of Ca in damaged compared to non-damaged leaves on the flower stem suggests that the damages to the flowers and the leaves is related to local deficiencies of Ca.

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