scholarly journals Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of Streptomyces sp. strain MJM 8637

2015 ◽  
Vol 22 (6) ◽  
pp. 744-751 ◽  
Author(s):  
Sung-Kwon Lee ◽  
Dong-Ryung Lee ◽  
Bong-Keun Choi ◽  
Sasikumar Arunachalam Palaniyandi ◽  
Seung Hwan Yang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Glutathione S Transferase ◽  
Streptomyces Sp ◽  
Anti Inflammation

Metabolite fingerprinting of novel Streptomyces UK-238 from the Himalayan forest

2020 ◽  
Vol 16 ◽  
Author(s):  
Nidhi Srivastava ◽  
Indira P. Sarethy
Keyword(s):  
Ethyl Acetate ◽  
16S Rdna ◽  
Retention Index ◽  
Ethyl Acetate Extract ◽  
Similarity Index ◽  
Antimicrobial Drug ◽  
16S Rdna Sequencing ◽  
Metabolite Fingerprinting ◽  
Streptomyces Sp ◽  
Rdna Sequencing

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

scholarly journals Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

2016 ◽  
Vol 19 (1) ◽  
pp. 37-43
Author(s):  
Md Anwarul Haque ◽  
Ashish Kumar Sarker ◽  
Md Ajijur Rahman ◽  
Md Aktar Uzzaman Chouduri ◽  
Md Anwar Ul Islam
Keyword(s):  
Antifungal Activity ◽  
Mortality Rate ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Small Scale ◽  
Dilution Method ◽  
Streptomyces Sp

To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50µg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40µg/ml and more where as LC50 was found to be 6.61µg/ml.Bangladesh Pharmaceutical Journal 19(1): 37-43, 2016

scholarly journals THE ANTIBACTERIAL COMPOUND COLLISMICIN A DERIVED FROM MARINE Streptomyces sp Q-629K

2010 ◽  
Vol 8 (3) ◽  
pp. 426-430
Author(s):  
Tutik Murniasih ◽  
Kyoko Adachi
Keyword(s):  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
13C Nmr ◽  
Active Compound ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Water System ◽  
Antimicrobial Compound ◽  
Streptomyces Sp

In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis .   Keywords: antimicrobial, collismycin A and marine Streptomyces sp

Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura

2020 ◽  
Author(s):  
Mandeep Kaur ◽  
Pooja Chadha ◽  
Sanehdeep Kaur ◽  
Amarjeet Kaur ◽  
Rajvir Kaur
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Ethyl Acetate ◽  
Ascorbate Peroxidase ◽  
Spodoptera Litura ◽  
Ethyl Acetate Extract ◽  
Schizophyllum Commune ◽  
Glutathione S Transferase ◽  
Insect Larvae ◽  
Detoxifying Enzymes

Abstract Background: In a search for the mechanism of action of an endophytic fungus Schizophyllum commune against Spodoptera litura, the effect of its ethyl acetate extract was judged on antioxidant and detoxifying enzymes as well as on morphology of haemocytes. Results: Ethyl acetate extract of S.commune was administrated to the larvae of S.litura using artificial diet method having concentration 276.54µg/ml (LC50 of fungus). The effect on antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) was observed using haemolymph and midgut of insect larvae for different time intervals (24, 48, 72 and 96). In additionally, haemocytes morphology was also studied using Scanning Electron Microscopy (SEM) after 96hr. In particular, to resist the toxicity, the activities of the antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) significantly (p≤0.05) increased in both the tissues of treated larvae as compared to control. The exposed group revealed various deformities in haemocytes of S.litura like breakage in the cell membrane, cytoplasmic leakage and appearance of strumae etc. Conclusion: This study provides important information regarding the oxidative stress causing potential and immunosuppressant nature of S.commune against S.litura and elucidates the patterns of antioxidant and detoxifying enzymes activities and changes in haemocytes in the susceptible larvae.

scholarly journals Unusual 2(1H)-Pyrazinones Isolated from a Culture of a Brazilian Marine-Derived Streptomyces sp

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Sérgio S. Thomasi ◽  
Ana C. Granato ◽  
Luis H. Romano ◽  
Liene Dhooghe ◽  
Eduardo S. P. do Nascimento ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Chemical Analysis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Streptomyces Strain ◽  
Streptomyces Sp

Four new secondary metabolites, giovaninones A-D (1–4), were isolated from an ethyl acetate extract of a culture of a marine-derived Streptomyces strain designated SS99BA-2. Chemical analysis was completely conducted in a coupled automated LC-SPE system with the use of a cryogenic NMR probehead and HRMS. The application of this system to identify, purify and elucidate all the structures is described.

scholarly journals EVALUATION OF THE ANTIMALARIAL POTENTIAL OF STREPTOMYCES SP.

2018 ◽  
Vol 11 (2) ◽  
pp. 354
Author(s):  
Sweetline C ◽  
Usha R
Keyword(s):  
Ethyl Acetate ◽  
Tamil Nadu ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Antiplasmodial Activity ◽  
Control Group ◽  
Standard Drug ◽  
Streptomyces Sp ◽  
Mangrove Soil ◽  
Dose Dependent

Objective: Isolate, screen and identify Streptomyces sp. from mangrove soil from pichavaram, Tamil Nadu, India, and study the molecular identification of selected Streptomyces sp. and check the antimalarial activity for the purified compound.Methods: The16SrRNA secondary structure and the restriction sites of KMA08 were predicted using Genbank online software, respectively. Antiplasmodial activity of the 80% Ethyl acetate extract of Streptomyces sp. against chloroquine-sensitive Plasmodium berghei in mice using the 4 days suppression test was conducted. A total of 30 mice assigned to 5 groups of 6 animals each were infected with chloroquine-sensitive (P. berghei) intraperitoneally. The Ethyl acetate extract (10, 20, and 30 ml/kg), standard drug (chloroquine, 10 mg/kg) and distilled water were administered orally daily for the treatment period. Percent Parasitemia was determined on the 5th day from Giemsa stained smears obtained from tail vein and percent parasitemia suppression was calculated. Daily measurement of rectal temperature was also taken while body weight and packed cell volume were recorded on day 0 and 5.Results: Results showed the extract produced a dose-dependent reduction in parasite density compared to the control group. Percept parasitemia calculation revealed 21.3, 65.3, and 80.5% inhibition at 10, 20, and 30 ml/kg of the extract, respectively.Conclusion: The study revealed the present work indicated that Streptomyces sp. has as promising antiplasmodial activity against chloroquine sensitive P. berghei in a dose-dependent. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.

scholarly journals CYTOTOXIC EFFECTS OF ETHYL ACETATE FRACTIONS FROM SECONDARY METABOLITES OF STREPTOMYCES SP. GMY01 ON HUMAN BREAST CANCER MCF7 CELL LINES

2017 ◽  
Vol 10 (14) ◽  
pp. 5
Author(s):  
Sista Werdyani ◽  
Nastiti Wijayanti ◽  
Annisa Fitria ◽  
Sari Rahmawati
Keyword(s):  
Breast Cancer ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Human Breast Cancer ◽  
Cytotoxic Effect ◽  
Mcf7 Cell ◽  
Ethyl Acetate Extract ◽  
Human Breast ◽  
Streptomyces Sp ◽  
Breast Cancer Mcf7 Cell

Objective: This research aimed to fractionate the ethyl acetate extract from secondary metabolites of Streptomyces sp. GMY01 and to identify which fraction contains cytotoxic active compounds against human breast cancer MCF7 cell lines.Methods: Secondary metabolites were obtained from fermentation of Streptomyces Sp. GMY01 for 15 d. The supernatant containing these secondary metabolites was extracted through partition using ethyl acetate as the solvent. Fractionation of the ethyl acetate extract was conducted via column chromatography using silica gel as the solid phase while the gradient mobile phase consisted of n-hexane, ethyl acetate, and methanol. The cytotoxicity of each fraction was calculated using MTT-assay.Results: The ethyl acetate extract could be separated into 9 fractions using column chromatography. The cytotoxic effect of each fraction differed from each other. The smallest IC50 value was obtained from fraction 4. Further investigation should be conducted to discover the active anticancer compound. The active compound with cytotoxic effect was found in fraction 4 because of the highest IC50 value.Conclusion: This fraction is potential to be investigated more deeply as anticancer, especially for breast cancer.

scholarly journals Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21

2019 ◽  
Vol 16 (1) ◽  
pp. 1-10
Author(s):  
Diani Mentari ◽  
Mirtani Naima ◽  
Riska Wulansari ◽  
Jaka Widada ◽  
Tri Rini Nuringtyas ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Host Cells ◽  
Extraction Technique ◽  
Active Compounds ◽  
Streptomyces Sp ◽  
Separatory Funnel

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells

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