Antimicrobial and Anticancer Activities of Ethyl Acetate Extract of Co-culture of Streptomyces sp. ANAM-5 and AIAH-10 Isolated From Mangrove Forest of Sundarbans, Bangladesh

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

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In vitro Insecticidal and Time-Kill Profile of Ethyl Acetate Extract of Marine Streptomyces sp. Isolated from Sundarbans, Bangladesh

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v17i2.22332 ◽

2015 ◽

Vol 17 (2) ◽

pp. 151-156 ◽

Cited By ~ 1

Author(s):

Md Anwarul Haque ◽

Ashish Kumar Sarker ◽

Mohammad Sayful Islam ◽

Md Ajijur Rahman ◽

Md Akter Uzzaman Chouduri ◽

...

Keyword(s):

Ethyl Acetate ◽

Mangrove Forest ◽

Bacterial Strains ◽

Streptomyces Species ◽

Streptomyces Sp ◽

Cell Counts ◽

Wide Range ◽

Marine Streptomyces ◽

Time Kill

The marine soil and sediment samples were collected from different locations of mangrove forest Sundarbans, Bangladesh the largest tidal halophytic mangrove forest in the world. A total of twenty nine Actinomycete strains (AIAH-1 to AIAH-29) were isolated by serial dilution method using isolation media. Among twenty nine strains, AIAH-10 was selected for further study due to its potent antibacterial activity against a wide range of pathogenic bacterial strains. On the basis of morphological, cultural and biochemical studies, the strain AIAH-10 was assigned to Streptomyces sp. The present study was designed to investigate the in vitro insecticidal and time-kill profile of ethyl acetate extracts of marine Streptomyces sp. A dose dependent mortality was observed against the larvae of Sitophilus oryzae. The larval mortality was recorded as 100% in the concentration of 80 mg/ml and higher concentrations, LC50 was found as 11.48 mg/ml. The minimum inhibitory concentration was recorded as 8 to 32 mg/ml against six different pathogenic bacterial strains. Average Log10 reductions in viable cell counts for the extracts ranged from 1.91 Log10 and 2.86 Log10 cfu/mL after 3 h interaction and 2.10 Log10 and 2.93 Log10 after 6 h interaction at MIC, 2 × MIC, 3 × MIC and 4 × MIC concentrations. This investigation reveals that the Streptomyces species isolated from Sundarbans, Bangladesh may be interesting source for the isolation of potent bioactive compounds. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22332 Bangladesh Pharmaceutical Journal 17(2): 151-156, 2014

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Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of Streptomyces sp. strain MJM 8637

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2015.04.003 ◽

2015 ◽

Vol 22 (6) ◽

pp. 744-751 ◽

Cited By ~ 1

Author(s):

Sung-Kwon Lee ◽

Dong-Ryung Lee ◽

Bong-Keun Choi ◽

Sasikumar Arunachalam Palaniyandi ◽

Seung Hwan Yang ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Glutathione S Transferase ◽

Streptomyces Sp ◽

Anti Inflammation

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Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

Bangladesh Pharmaceutical Journal ◽

10.3329/bpj.v19i1.29235 ◽

2016 ◽

Vol 19 (1) ◽

pp. 37-43

Author(s):

Md Anwarul Haque ◽

Ashish Kumar Sarker ◽

Md Ajijur Rahman ◽

Md Aktar Uzzaman Chouduri ◽

Md Anwar Ul Islam

Keyword(s):

Antifungal Activity ◽

Mortality Rate ◽

Ethyl Acetate ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Bioactive Molecules ◽

Diffusion Method ◽

Small Scale ◽

Dilution Method ◽

Streptomyces Sp

To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50µg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40µg/ml and more where as LC50 was found to be 6.61µg/ml.Bangladesh Pharmaceutical Journal 19(1): 37-43, 2016

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THE ANTIBACTERIAL COMPOUND COLLISMICIN A DERIVED FROM MARINE Streptomyces sp Q-629K

Indonesian Journal of Chemistry ◽

10.22146/ijc.21600 ◽

2010 ◽

Vol 8 (3) ◽

pp. 426-430

Author(s):

Tutik Murniasih ◽

Kyoko Adachi

Keyword(s):

Ethyl Acetate ◽

Secondary Metabolite ◽

13C Nmr ◽

Active Compound ◽

Chemical Structure ◽

Ethyl Acetate Extract ◽

Water System ◽

Antimicrobial Compound ◽

Streptomyces Sp

In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis . Keywords: antimicrobial, collismycin A and marine Streptomyces sp

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Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes tch4 extract

Archives of Biological Sciences ◽

10.2298/abs210320017t ◽

2021 ◽

pp. 17-17

Author(s):

Janpen Tangjitjaroenkun ◽

Wanchai Pluempanupat ◽

Rungnapa Tangchitcharoenkhul ◽

Waraporn Yahayo ◽

Roongtawan Supabphol

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Biological Activities ◽

Radical Scavenging ◽

Bioactive Metabolites ◽

Total Phenolic ◽

Anticancer Activities ◽

Antioxidant Power ◽

Dry Extract

The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 were 107.20?2.57 mg gallic acid equivalent (GAE)/g and 44.91?0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?0.37 ?g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.

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Unusual 2(1H)-Pyrazinones Isolated from a Culture of a Brazilian Marine-Derived Streptomyces sp

Natural Product Communications ◽

10.1177/1934578x1400900913 ◽

2014 ◽

Vol 9 (9) ◽

pp. 1934578X1400900

Author(s):

Sérgio S. Thomasi ◽

Ana C. Granato ◽

Luis H. Romano ◽

Liene Dhooghe ◽

Eduardo S. P. do Nascimento ◽

...

Keyword(s):

Secondary Metabolites ◽

Chemical Analysis ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Streptomyces Strain ◽

Streptomyces Sp

Four new secondary metabolites, giovaninones A-D (1–4), were isolated from an ethyl acetate extract of a culture of a marine-derived Streptomyces strain designated SS99BA-2. Chemical analysis was completely conducted in a coupled automated LC-SPE system with the use of a cryogenic NMR probehead and HRMS. The application of this system to identify, purify and elucidate all the structures is described.

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EVALUATION OF THE ANTIMALARIAL POTENTIAL OF STREPTOMYCES SP.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i2.21230 ◽

2018 ◽

Vol 11 (2) ◽

pp. 354

Author(s):

Sweetline C ◽

Usha R

Keyword(s):

Ethyl Acetate ◽

Tamil Nadu ◽

Ethyl Acetate Extract ◽

Antimalarial Activity ◽

Antiplasmodial Activity ◽

Control Group ◽

Standard Drug ◽

Streptomyces Sp ◽

Mangrove Soil ◽

Dose Dependent

Objective: Isolate, screen and identify Streptomyces sp. from mangrove soil from pichavaram, Tamil Nadu, India, and study the molecular identification of selected Streptomyces sp. and check the antimalarial activity for the purified compound.Methods: The16SrRNA secondary structure and the restriction sites of KMA08 were predicted using Genbank online software, respectively. Antiplasmodial activity of the 80% Ethyl acetate extract of Streptomyces sp. against chloroquine-sensitive Plasmodium berghei in mice using the 4 days suppression test was conducted. A total of 30 mice assigned to 5 groups of 6 animals each were infected with chloroquine-sensitive (P. berghei) intraperitoneally. The Ethyl acetate extract (10, 20, and 30 ml/kg), standard drug (chloroquine, 10 mg/kg) and distilled water were administered orally daily for the treatment period. Percent Parasitemia was determined on the 5th day from Giemsa stained smears obtained from tail vein and percent parasitemia suppression was calculated. Daily measurement of rectal temperature was also taken while body weight and packed cell volume were recorded on day 0 and 5.Results: Results showed the extract produced a dose-dependent reduction in parasite density compared to the control group. Percept parasitemia calculation revealed 21.3, 65.3, and 80.5% inhibition at 10, 20, and 30 ml/kg of the extract, respectively.Conclusion: The study revealed the present work indicated that Streptomyces sp. has as promising antiplasmodial activity against chloroquine sensitive P. berghei in a dose-dependent. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.

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Semecarpus anacardium Linn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor bearing mice

10.1101/2020.01.08.898940 ◽

2020 ◽

Author(s):

Rajesh Kumar Singh ◽

Amit Ranjan ◽

Ruchita Tripathi ◽

Sumit Singh Verma ◽

Vinamra Sharma ◽

...

Keyword(s):

Ethyl Acetate ◽

Cancer Cells ◽

Cancer Cell ◽

Ethyl Acetate Extract ◽

Dependent Manner ◽

Microbial Infection ◽

Mice Model ◽

Anticancer Activities ◽

Semecarpus Anacardium ◽

Tumor Bearing

ABSTRACTSemecarpus anacardium Linn. is a commonly used Ayurvedic medicinal plant which nuts have been described in Ayurveda and Sidda system of medicine to treat clinical ailments such as vitiligo, inflamation, microbial infection, geriatric problem, baldness and neuro related problems. In this study, anti-cancer activity of the leaves of Semecarpus anacardium Linn was evaluated for future drug development. The phytochemical screening was done by GC-MS analysis, cytotoxicity was examined using MTT assay, mode of cell death was evaluated by fluorescence microscopy and finally antitumor activity was determined in EAC cell induced tumor bearing mice. The ethyl acetate extract from the leaves of the plant induced cytotoxicity in cancer cells in a dose dependent manner (IC50: 0.57 μg/ml in MCF-7 cells) in different cancer cell lines. The non-malignant cells were relatively insensitive to the extract. The staining with acridine orange, ethidium bromide and DAPI confirmed that the extract induced apoptosis in cancer cells. Furthermore, the extract induced cell cycle arrest at G1 phase and suppressed cancer cell migration. An oral administration of the extract suppressed the tumor growth in mice model bearing ehrlich ascites carcinoma cells. The ethyl acetate extract was also found to prolong the survival of tumor bearing mice.Overall, these observations suggest the anticancer activities of the ethyl acetate extract of the leaves of S. anacardium. The study opens a new window to examine the phytochemical constituents from the leaves of the plant responsible for the anticancer activities.

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CYTOTOXIC EFFECTS OF ETHYL ACETATE FRACTIONS FROM SECONDARY METABOLITES OF STREPTOMYCES SP. GMY01 ON HUMAN BREAST CANCER MCF7 CELL LINES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017v10s3.21351 ◽

2017 ◽

Vol 10 (14) ◽

pp. 5

Author(s):

Sista Werdyani ◽

Nastiti Wijayanti ◽

Annisa Fitria ◽

Sari Rahmawati

Keyword(s):

Breast Cancer ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Human Breast Cancer ◽

Cytotoxic Effect ◽

Mcf7 Cell ◽

Ethyl Acetate Extract ◽

Human Breast ◽

Streptomyces Sp ◽

Breast Cancer Mcf7 Cell

Objective: This research aimed to fractionate the ethyl acetate extract from secondary metabolites of Streptomyces sp. GMY01 and to identify which fraction contains cytotoxic active compounds against human breast cancer MCF7 cell lines.Methods: Secondary metabolites were obtained from fermentation of Streptomyces Sp. GMY01 for 15 d. The supernatant containing these secondary metabolites was extracted through partition using ethyl acetate as the solvent. Fractionation of the ethyl acetate extract was conducted via column chromatography using silica gel as the solid phase while the gradient mobile phase consisted of n-hexane, ethyl acetate, and methanol. The cytotoxicity of each fraction was calculated using MTT-assay.Results: The ethyl acetate extract could be separated into 9 fractions using column chromatography. The cytotoxic effect of each fraction differed from each other. The smallest IC50 value was obtained from fraction 4. Further investigation should be conducted to discover the active anticancer compound. The active compound with cytotoxic effect was found in fraction 4 because of the highest IC50 value.Conclusion: This fraction is potential to be investigated more deeply as anticancer, especially for breast cancer.

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